The sphingosine kinase 1/sphingosine‐1‐phosphate pathway mediates COX‐2 induction and PGE 2 production in response to TNF‐α

Abstract

In this study we addressed the role of sphingolipid metabolism in the inflammatory response. In a L929 fibroblast model, tumor necrosis factor‐α (TNF) induced prostaglandin E 2 (PGE 2 ) production by 4 h and cyclooxygenase‐2 (COX‐2) induction as early as 2 h. This TNF‐induced PGE 2 production was inhibited by NS398, a COX‐2 selective inhibitor. GC‐MS analysis revealed that only COX‐2‐generated prostanoids were produced in response to TNF, thus providing further evidence of COX‐2 selectivity. As sphingolipids have been implicated in mediating several actions of TNF, their role in COX‐2 induction and PGE 2 production was evaluated. Sphingosine‐1‐phosphate (S1P) induced both COX‐2 and PGE 2 in a dose‐responsive manner with an apparent ED 50 of 100–300 nM. The related sphingolipid sphingosine also induced PGE 2 , though with much less efficacy. TNF induced a 3.5‐fold increase in sphingosine‐1‐phosphate levels at 10 min that rapidly returned to baseline by 40 min. Small interfering RNAs (siRNAs) directed against mouse SK1 decreased (typically by 80%) SK1 protein and inhibited TNF‐induced SK activity. Treatment of cells with RNAi to SK1 but not SK2 almost completely abolished the ability of TNF to induce COX‐2 or generate PGE 2 . By contrast, cells treated with RNAi to S1P lyase or S1P phosphatase enhanced COX‐2 induction leading to enhanced generation of PGE 2 . Treatment with SK1 RNAi also abolished the effects of exogenous sphin‐gosine and ceramide on PGE 2 , revealing that the action of sphingosine and ceramide are due to intracellular metabolism into S1P. Collectively, these results provide novel evidence that SK1 and S1P are necessary for TNF to induce COX‐2 and PGE 2 production. Based on these findings, this study indicates that SK1 and S1P could be implicated in pathological inflammatory disorders and cancer.—Pettus, B. J., Bielawski, J., Porcelli, A. M., Reames, D. L., Johnson, K. R., Morrow, J., Chalfant, C. E., Obeid, L. M., Hannun, Y A. The sphingosine kinase 1/sphingosine‐1‐phosphate pathway mediates COX‐2 induction and PGE 2 production in response to TNF‐α, FASEB J. , 17, 1411–1421 (2003)