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Reactive glial cells: increased stiffness correlates with increased intermediate filament expression
Abstract
Increased stiffness of reactive glial cells may impede neurite growth and contribute to the poor regenerative capabilities of the mammalian central nervous system. We induced reactive gliosis in rodent retina by ischemia-reperfusion and assessed intermediate filament (IF) expression and the viscoelastic properties of dissociated single glial cells in wild-type mice, mice lacking glial fibrillary acidic protein and vimentin (GFAP(-/-)Vim(-/-)) in which glial cells are consequently devoid of IFs, and normal Long-Evans rats. In response to ischemia-reperfusion, glial cells stiffened significantly in wild-type mice and rats but were unchanged in GFAP(-/-)Vim(-/-) mice. Cell stiffness (elastic modulus) correlated with the density of IFs. These results support the hypothesis that rigid glial scars impair nerve regeneration and that IFs are important determinants of cellular viscoelasticity in reactive glia. Thus, therapeutic suppression of IF up-regulation in reactive glial cells may facilitate neuroregeneration.
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Cites
Müller cells in the healthy and diseased retina
Bringmann, A., Pannicke, T., Grosche, J., Francke, M., Wiedemann, P., Skatchkov, S. N., Osborne, N. N., & Reichenbach, A. (2006) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2006.05.003 · Cited by 1,967
Self-citationTripartite synapses: astrocytes process and control synaptic information
Perea, G., Navarrete, M., & Araque, A. (2009) · Trends in Neurosciences · doi:10.1016/j.tins.2009.05.001 · Cited by 1,820
Astrocyte activation and reactive gliosis
Pekny, M., & Nilsson, M. (2005) · Glia · doi:10.1002/glia.20207 · Cited by 1,611
Self-citationNeurite branching on deformable substrates
Flanagan, L. A., Ju, Y. S., Marg, B., Osterfield, M., & Janmey, P. A. (2002) · Neuroreport · doi:10.1097/00001756-200212200-00007 · Cited by 759
Wilhelmsson, U., Bushong, E. A., Price, D. L., Smarr, B. L., Phung, V., Terada, M., Ellisman, M. H., & Pekny, M. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0602841103 · Cited by 643 · cited 1×
“IFs and IF proteins are commonly used as markers of normal and reactive glial cells (9, 24, 27), but their physiological and pathophysiological functions are poorly understood (10).”
BackgroundSelf-citationViscoelastic properties of individual glial cells and neurons in the CNS
Lu, Y., Franze, K., Seifert, G., Steinhäuser, C., Kirchhoff, F., Wolburg, H., Guck, J. R., Janmey, P. A., Wei, E.-Q., Käs, J. A., et al. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0606150103 · Cited by 580
Self-citationScanning Probe-Based Frequency-Dependent Microrheology of Polymer Gels and Biological Cells
Mahaffy, R. E., Shih, C.-K. K., MacKintosh, F. C., & Käs, J. A. (2000) · Physical Review Letters · doi:10.1103/physrevlett.85.880 · Cited by 509
Self-citationMahaffy, R. E., Park, S., Gerde, E., Käs, J. A., & Shih, C.-K. K. (2004) · Biophysical Journal · doi:10.1016/s0006-3495(04)74245-9 · Cited by 460
Self-citationWilhelmsson, U., Li, L., Pekna, M., Berthold, C.-H., Blom, S., Eliasson, C., Renner, O., Bushong, E., Ellisman, M., Morgan, T. E., et al. (2004) · The Journal of Neuroscience · doi:10.1523/jneurosci.0820-04.2004 · Cited by 455
Self-citationAstrocytes and the regulation of cerebral blood flow
Koehler, R. C., Roman, R. J., & Harder, D. R. (2009) · Trends in Neurosciences · doi:10.1016/j.tins.2008.11.005 · Cited by 439
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Wang, R., & Sarntinoranont, M. (2018) · Journal of the mechanical behavior of biomedical materials/Journal of mechanical behavior of biomedical materials · doi:10.1016/j.jmbbm.2018.08.043 · Cited by 19
Eide, P. K. (2022) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2022.981399 · Cited by 18
Fuhs, T., Reuter, L., Vonderhaid, I., Claudepierre, T., & Käs, J. A. (2012) · Cytoskeleton · doi:10.1002/cm.21080 · Cited by 18
Self-citationDvoriantchikova, G., Santos, A. R. C., Danek, D. J., Dvoriantchikova, X., & Ivanov, D. (2014) · European Journal of Neuroscience · doi:10.1111/ejn.12603 · Cited by 18
Reichenbach, A., & Bringmann, A. (2015) · Colloquium Series on Neuroglia in Biology and Medicine From Physiology to Disease · doi:10.4199/c00122ed1v01y201412ngl003 · Cited by 18
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Laterza, C., Uoshima, N., Tornero, D., Wilhelmsson, U., Stokowska, A., Ge, R., Pekny, M., Lindvall, O., & Kokaia, Z. (2018) · PLoS ONE · doi:10.1371/journal.pone.0192118 · Cited by 15
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Antonovaité, N., van Wageningen, T. A., Paardekam, E. J., van Dam, A., & Iannuzzi, D. (2020) · Journal of the mechanical behavior of biomedical materials/Journal of mechanical behavior of biomedical materials · doi:10.1016/j.jmbbm.2020.103783 · Cited by 14
Lahmar, I., Pfaff, A. W., Marcellin, L., Sauer, A., Moussa, A., Babba, H., & Candolfi, E. (2014) · Experimental Parasitology · doi:10.1016/j.exppara.2014.06.006 · Cited by 15
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Ren, Z., Zhang, C.-X., Zheng, Y., Chen, C.-A., Dan-Chen, Lan, X., Yan, X., Liu, Y., He, Y.-H., Cheng, J.-L., et al. (2025) · Phytomedicine · doi:10.1016/j.phymed.2025.156696 · Cited by 10
Åkerström, B., Cederlund, M., Bergwik, J., Manouchehrian, O., Arnér, K. M., Holmgren-Taylor, I., Ghosh, F. K., & Taylor, L. (2017) · Current Eye Research · doi:10.1080/02713683.2016.1254247 · Cited by 9
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Winkler, U., Hirrlinger, P., Sestu, M., Wilhelm, F., Besser, S., Zemljic-Harpf, A. E., Ross, R., Bornschein, G., Krügel, U., Ziegler, W., et al. (2013) · Glia · doi:10.1002/glia.22495 · Cited by 3
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Sean Sanders DVM, P. (2025) · doi:10.1002/9781394245642.ch5 · Cited by 1
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Li, B., Fu, X., Niu, X., Fan, Y., Xu, W., Zhao, X. X., & Pu, J. (2015) · Genetics and Molecular Research · doi:10.4238/2015.january.30.7
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Graca, A. B. (2017) · UCL Discovery (University College London)
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Weir, N. (2019) · Nottingham Trent University's Institutional Repository (Nottingham Trent Repository)
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Yin, Z., Ge, L., Cha, Z., Gao, H., Luodan, A., Zeng, Y., Huang, X., Cheng, X., Yao, K., Tao, Z., et al. (2024) · Figshare · doi:10.6084/m9.figshare.25302992
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Metadata
- Authors
- Lu, Y., Iandiev, I., Hollborn, M., Körber, N., Ulbricht, E., Hirrlinger, P. G., Pannicke, T., Wei, E., Bringmann, A., Wolburg, H., Wilhelmsson, U., Pekny, M., Wiedemann, P., Reichenbach, A., & Käs, J. A.
- Affiliations
- Zhejiang University (CN)
- Leipzig University (DE)
- University of Tübingen (DE)
- University of Gothenburg (SE)
- Corresponding author
- Reichenbach, Andreas
- ORCID
- Venue
- The FASEB Journal
- Publisher
- Wiley
- Published
- 2010-10-25
- Volume
- 25
- Issue
- 2
- Pages
- 624-631
- ISSN
- 0892-6638 (ISSN-L)
- 1530-6860
- OpenAlex
- W1977341002
- Cited by
- 172
- References
- 34
- Citations by year
- 2026: 3 · 2025: 11 · 2024: 7 · 2023: 6 · 2022: 7 · 2021: 15 · 2020: 15 · 2019: 14 · 2018: 11 · 2017: 12 · 2016: 10 · 2015: 17 · 2014: 10 · 2013: 19 · 2012: 12
- FWCI
- 4.76
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The FASEB Journal (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft (SPP1172)
- National Natural Science Foundation of China (30700150)
- Deutscher Akademischer Austauschdienst (Y2080105)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
- Health Sciences › Medicine › Ophthalmology › Glaucoma and retinal disorders
- Keywords
- reactive glial cells, cell stiffness, intermediate filaments, glial fibrillary acidic protein, vimentin, reactive gliosis, retinal ischemia-reperfusion, viscoelasticity, glial scar, nerve regeneration
- MeSH terms
- Animals, Biomechanical Phenomena, Gene Expression Regulation, Glial Fibrillary Acidic Protein, Gliosis, Intermediate Filaments, Nerve Tissue Proteins, Neuroglia, Vimentin, Reperfusion Injury, Mice, Knockout, Rats, Long-Evans, Mice, Rats
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