Activation of satellite cells and the regeneration of human skeletal muscle are expedited by ingestion of nonsteroidal anti-inflammatory medication
Open accessAbstract
With this study we investigated the role of nonsteroidal anti-inflammatory drugs (NSAIDs) in human skeletal muscle regeneration. Young men ingested NSAID [1200 mg/d ibuprofen (IBU)] or placebo (PLA) daily for 2 wk before and 4 wk after an electrical stimulation-induced injury to the leg extensor muscles of one leg. Muscle biopsies were collected from the vastus lateralis muscles before and after stimulation (2.5 h and 2, 7, and 30 d) and were assessed for satellite cells and regeneration by immunohistochemistry and real-time RT-PCR, and we also measured telomere length. After injury, and compared with PLA, IBU was found to augment the proportion of ActiveNotch1(+) satellite cells at 2 d [IBU, 29 ± 3% vs. PLA, 19 ± 2% (means ± sem)], satellite cell content at 7 d [IBU, 0.16 ± 0.01 vs. PLA, 0.12 ± 0.01 (Pax7(+) cells/fiber)], and to expedite muscle repair at 30 d. The PLA group displayed a greater proportion of embryonic myosin(+) fibers and a residual ∼2-fold increase in mRNA levels of matrix proteins (all P < 0.05). Endomysial collagen was also elevated with PLA at 30 d. Minimum telomere length shortening was not observed. In conclusion, ingestion of NSAID has a potentiating effect on Notch activation of satellite cells and muscle remodeling during large-scale regeneration of injured human skeletal muscle.-Mackey, A. L., Rasmussen, L. K., Kadi, F., Schjerling, P., Helmark, I. C., Ponsot, E., Aagaard, P., Durigan, J. L. Q., Kjaer, M. Activation of satellite cells and the regeneration of human skeletal muscle are expedited by ingestion of nonsteroidal anti-inflammatory medication.
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n-3 PUFAs, via their anti-inflammatory properties, could promote a systemic environment that improves satellite cell responsiveness to injury during aging by attenuating systemic inflammation, in a similar manner to nonsteroidal anti-inflammatory drugs (NSAIDs).
“n-3 PUFAs with their anti-inflammatory properties could promote a systemic environment that improves satellite cell responsiveness to injury during aging by attenuating systemic inflammation in a similar manner to nonsteroidal anti-inflammatory drugs (NSAIDs) [118,119,120].”
From Potential Roles of n-3 PUFAs during Skeletal Muscle Growth and Regeneration (2018) · cites this paper as [119]
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Muscle damage and inflammation during recovery from exercise
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InfluentialIbuprofen alters human testicular physiology to produce a state of compensated hypogonadism
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“Exclusion criteria included smoking, diabetes, the use of non-steroidal anti-inflammatory drugs (NSAIDs) and/or statins, and a history of respiratory disease and/or any major orthopaedic disability.”
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Stupin, M., Kibel, A., Stupin, A., Selthofer-Relatić, K., Matić, A., Mihalj, M., Mihaljević, Z., Jukić, I., & Drenjančević, I. (2019) · Frontiers in Physiology · doi:10.3389/fphys.2019.01129 · Cited by 59 · see citing works
“Anti-inflammatory effects of n-3 PUFAs could promote muscle stem cell responsiveness to injury by attenuating systemic inflammation (Apolinário et al., 2015; Mackey et al., 2016).”
BackgroundMolecular indicators of denervation in aging human skeletal muscle
Soendenbroe, C., Heisterberg, M. F., Schjerling, P., Karlsen, A., Kjær, M., Andersen, J. L., & Mackey, A. L. (2019) · Muscle & Nerve · doi:10.1002/mus.26638 · Cited by 51 · see citing works
“Muscle injury in adult animals and humans also leads to a process of regeneration in which MHCe/n become transiently reexpressed.(9,14) Because such fibers are rarely observed in young individuals, it can be assumed that these immature myosins are gradually removed in the following months.”
BackgroundResultIndirect Structural Muscle Injuries of Lower Limb: Rehabilitation and Therapeutic Exercise
Palermi, S., Massa, B., Vecchiato, M., Mazza, F., Blasiis, P. D., Romano, A. M., Salvatore, M. G. D., Valle, E. D., Tarantino, D., Ruosi, C., et al. (2021) · Journal of Functional Morphology and Kinesiology · doi:10.3390/jfmk6030075 · Cited by 50 · see citing works
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BackgroundSatellite cell activation and apoptosis in skeletal muscle from severely burned children
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“…data on skeletal muscle injury and satellite cell activation in clinical studies is available, and existing literature largely utilizes exercise-induced muscle damage as an injury proxy (O’Reilly et al. 2008; Mackey et al. 2016) or studies the acute effect of muscle tears (Gigliotti et al. 2015).”
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“, 2016) or on the mRNA levels of Cd68 (Mackey et al., 2016), suggesting that the effects of NSAIDs on human muscle function are not necessarily mediated by the regulation of immune cells.”
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“Images were analysed using the ImageJ software, using the thresholding function to determine the Raw Integrated Density to take into account the area and staining intensity of the stained pixels, as described previously (Mackey et al., 2016).”
MethodNederveen, J. P., Ibrahim, G., Fortino, S. A., Snijders, T., Kumbhare, D., & Parise, G. (2020) · Applied Physiology, Nutrition, and Metabolism · doi:10.1139/apnm-2019-0263 · Cited by 42 · see citing works
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Aging is associated with an altered macrophage response during human skeletal muscle regeneration
Ahmadi, M., Karlsen, A., Mehling, J., Soendenbroe, C., Mackey, A. L., & Hyldahl, R. D. (2022) · Experimental Gerontology · doi:10.1016/j.exger.2022.111974 · Cited by 39 · see citing works
“Our prior study, using a similar electrical stimulation protocol (Mackey et al., 2016) showed widespread myofiber necrosis and regenerative activity (i.e. satellite cell proliferation) 7 days following electrical stimulation.”
InfluentialRegenerating Myofibers after an Acute Muscle Injury: What Do We Really Know about Them?
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“Non-steroidal anti-inflammatory drug (NSAID) prescription in the early postinjury period, especially in the case of a medium to severe MI (II°–III°), can be considered as a justified medical treatment.(128) It should be remembered, however, that there is still no evidence of the benefits and the adverse effects that t…”
BackgroundPavis, G. F., Jameson, T. S. O., Dirks, M. L., Lee, B. P., Abdelrahman, D. R., Murton, A. J., Porter, C., Alamdari, N., Mikus, C. R., Wall, B. T., et al. (2021) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00454.2020 · Cited by 37 · see citing works
Jensen, S. M., Bechshøft, C. J. L., Heisterberg, M. F., Schjerling, P., Andersen, J. L., Kjær, M., & Mackey, A. L. (2020) · Frontiers in Physiology · doi:10.3389/fphys.2020.00811 · Cited by 33 · see citing works
“The timing of this regulation aligns with other findings from our laboratory demonstrating that ECM remodeling increases at later (>7 days) time points after exercise or injury (Mackey et al., 2016; Mackey and Kjaer, 2017; Karlsen et al., 2020).”
ResultNonimmune mechanisms in idiopathic inflammatory myopathies
Loredo, M., Zampieri, S., Franco, C., Ghirardello, A., Doria, A., & Gatto, M. (2020) · Current Opinion in Rheumatology · doi:10.1097/bor.0000000000000748 · Cited by 28 · see citing works
“1a) [39,40] and can be expressed also by muscle satellite cells and by regenerating myofibers together with the embryonic isoform of myosin heavy chain (embMHC) as part of the developmental program of skeletal muscle growth that is recapitulated during regeneration [35,41].”
BackgroundBjørnsen, T., Wernbom, M., Paulsen, G., Berntsen, S., Brankovic, R., Stålesen, H., Sundnes, J., & Raastad, T. (2021) · Scandinavian Journal of Medicine and Science in Sports · doi:10.1111/sms.13952 · Cited by 27 · see citing works
“03; Block2: 14 [13, 16] vs 10 [7, 13], p = 0.”
D’Lugos, A. C., Patel, S. H., Ormsby, J. C., Curtis, D., Fry, C. S., Carroll, C. C., & Dickinson, J. M. (2018) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00922.2017 · Cited by 26 · see citing works
“subtle alterations in Myostatin and MYF6 expression induced by APAP consumption are, perhaps, indicative of suppressed myogenesis (36, 52), which would be consistent with previous findings with ibuprofen (28) and indomethacin (27, 35).”
ResultIsenmann, E., Blume, F., Bizjak, D. A., Hundsdörfer, V., Pagano, S., Schibrowski, S., Simon, W., Schmandra, L., & Diel, P. R. (2019) · Nutrients · doi:10.3390/nu11040744 · Cited by 26 · see citing works
“Molecular mechanisms involved in muscle recovery include a modulation of protein synthesis and protein breakdown [6], an inhibition of the inflammatory response, and the activation of satellite cells [7].”
BackgroundAncel, S., Michaud, J., Migliavacca, E., Jomard, C., Fessard, A., Garcia, P., Karaz, S., Raja, S., Jacot, G. E., Desgeorges, T., et al. (2024) · Journal of Clinical Investigation · doi:10.1172/jci163648 · Cited by 25 · see citing works
“…MuSCs with safe nutritional solutions is particularly relevant to accelerate physiological muscle recovery during MuSC-mediated repair of myofiber microdamage following exercise, sports-induced muscle tears and injuries, or following surgical procedures that mechanically rupture myofibers (5, 51).”
Molecular clocks, satellite cells, and skeletal muscle regeneration
Kahn, R. E., Dayanidhi, S., Lacham‐Kaplan, O., & Hawley, J. A. (2023) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00073.2023 · Cited by 23 · see citing works
Roberts, B. M., Geddis, A. V., & Matheny, R. W. (2024) · Journal of the International Society of Sports Nutrition · doi:10.1080/15502783.2024.2302046 · Cited by 22 · see citing works
Neuromuscular Electrical Stimulation Preserves Leg Lean Mass in Geriatric Patients
Karlsen, A., Cullum, C. K., Norheim, K. L., Scheel, F. U., Zinglersen, A. H., Vahlgren, J., Schjerling, P., Kjær, M., & Mackey, A. L. (2019) · Medicine & Science in Sports & Exercise · doi:10.1249/mss.0000000000002191 · Cited by 22 · see citing works
“Our laboratory has previously used the Ki67 antibody to label activated SCs (Pax7/ Ki67) cells after a severe muscle damaging protocol that resulted in a substantial increase in the number of SCs per fiber but only a modest increase in the proportion of Ki67/Pax7 cells 7 d after the intervention (40).”
BackgroundMethodΠαπανικολάου, Κ., Veskoukis, A. S., Draganidis, D., Baloyiannis, I., Deli, C. K., Poulios, A., Jamurtas, A. Z., & Fatouros, I. G. (2020) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2020.10.001 · Cited by 20 · see citing works
Langendorf, E. K., Klein, A., Drees, P., Rommens, P. M., Mattyasovszky, S. G., & Ritz, U. (2019) · Journal of Orthopaedic Research® · doi:10.1002/jor.24564 · Cited by 20 · see citing works
Liu, H., Cheema, U., & Player, D. J. (2025) · Photodiagnosis and Photodynamic Therapy · doi:10.1016/j.pdpdt.2025.104634 · Cited by 19 · see citing works
Dablainville, V., Mornas, A., Normand‐Gravier, T., Al‐Mulla, M., Papakostas, E., Olory, B. C. R., Fermín, T. M., Zampeli, F., Nader, N., Alhammoud, M., et al. (2025) · The Journal of Physiology · doi:10.1113/jp287777 · Cited by 19 · see citing works
Pax7+ Satellite Cells in Human Skeletal Muscle After Exercise: A Systematic Review and Meta-analysis
Dewi, L., Lin, Y., Nicholls, A., Condello, G., Huang, C., & Kuo, C. (2022) · Sports Medicine · doi:10.1007/s40279-022-01767-z · Cited by 20 · see citing works
“The range of Pax7+ cells of trained individuals was 3–33 cells per 100 myofibers [15, 19, 57, 63, 77] compared with 4–13 cells per 100 myofibers of the untrained individuals [4, 7, 8, 14, 38, 66, 67, 73, 76, 90, 92, 100].”
BackgroundReidy, P. T., Edvalson, L. T., McKenzie, A. I., Petrocelli, J. J., Mahmassani, Z. S., & Drummond, M. J. (2020) · Applied Physiology, Nutrition, and Metabolism · doi:10.1139/apnm-2020-0064 · Cited by 18 · see citing works
RNA sequencing and immunofluorescence of the myotendinous junction of mature horses and humans
Jakobsen, J. R., Schjerling, P., Svensson, R. B., Buhl, R., Carstensen, H., Koch, M., Krogsgaard, M. R., Kjær, M., & Mackey, A. L. (2021) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00218.2021 · Cited by 17 · see citing works
Boutrup, R. J., Farup, J., Vissing, K., Kjær, M., & Mikkelsen, U. R. (2018) · Rheumatology International · doi:10.1007/s00296-018-4028-y · Cited by 16 · see citing works
Sex Hormones and Satellite Cell Regulation in Women
Oxfeldt, M., Dalgaard, L. B., Farup, J., & Hansen, M. (2022) · Translational Sports Medicine · doi:10.1155/2022/9065923 · Cited by 16 · see citing works
“It is, however, worth considering that the in vivo SC cell cycle activity, and thus the expression of proliferation markers, is generally very low under homeostatic conditions [45, 103].”
BackgroundResistance Training with Co-ingestion of Anti-inflammatory Drugs Attenuates Mitochondrial Function
Cardinale, D. A., Lilja, M. R., Mandić, M., Gustafsson, T. P., Larsen, F. J., & Lundberg, T. R. (2017) · Frontiers in Physiology · doi:10.3389/fphys.2017.01074 · Cited by 15 · see citing works
“, 2009) including muscle protein metabolism and regeneration (Trappe et al., 2001, 2002; Mackey et al., 2016).”
BackgroundFusion of myofibre branches is a physiological feature of healthy human skeletal muscle regeneration
Højfeldt, G. W., Sorenson, T., Gonzales, A. G., Kjær, M., Andersen, J. L., & Mackey, A. L. (2023) · Skeletal Muscle · doi:10.1186/s13395-023-00322-2 · Cited by 14 · see citing works
“The muscle biopsies analysed in this study are a subset of samples collected during a larger study [25].”
BackgroundMethodEffect of Losartan on the Acute Response of Human Elderly Skeletal Muscle to Exercise
Heisterberg, M. F., Andersen, J. L., Schjerling, P., Bülow, J., Lauersen, J. B., Roeber, H. L., Kjær, M., & Mackey, A. L. (2017) · Medicine & Science in Sports & Exercise · doi:10.1249/mss.0000000000001438 · Cited by 14 · see citing works
“The inflammation markers (CD68, MMP2, CCL2) also responded acutely, as has been reported with resistance exercise (34,35), although no effect of AT1R blocking was observed.”
BackgroundPathogenesis of Musculotendinous and Fascial Injuries After Physical Exercise - Short Review
Mușat, C. L., Niculeț, E., Crăescu, M., Nechita, L., Iancu, L., Nechita, A., Voinescu, D.-C., & Bobeică, C. (2023) · International Journal of General Medicine · doi:10.2147/ijgm.s432749 · Cited by 13 · see citing works
“Skeletal striated muscles https://doi.org/10.2147/IJGM.S432749”
InfluentialCurrent Thoughts of Notch’s Role in Myoblast Regulation and Muscle-Associated Disease
Gerrard, J. C., Hay, J. P., Adams, R. N., Williams, J. C., Huot, J. R., Weathers, K. M., Marino, J. S., & Arthur, S. T. (2021) · International Journal of Environmental Research and Public Health · doi:10.3390/ijerph182312558 · Cited by 13 · see citing works
“Furthermore, ingestion of a non-steroidal anti-inflammatory drug (NSAID) following injurious electrically stimulated muscle contractions resulted in greater Notch signaling in activated satellite cells, suggesting that inhibiting the inflammatory process upregulates Notch and affects myogenesis [17].”
BackgroundPCM1 labeling reveals myonuclear and nuclear dynamics in skeletal muscle across species
Viggars, M. R., Owens, D. J., Stewart, C. E., Coirault, C., Mackey, A. L., & Jarvis, J. C. (2022) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00285.2022 · Cited by 12 · see citing works
Bernard, C., Jomard, C., Chazaud, B., & Gondin, J. (2023) · The FASEB Journal · doi:10.1096/fj.202201708rr · Cited by 12 · see citing works
Isolation and characterization of myogenic precursor cells from human cremaster muscle
Naldaiz‐Gastesi, N., Goicoechea, M., Aragón, I. M., Pérez‐López, V., Fuertes-Álvarez, S., Herrera‐Imbroda, B., de Munaín, A. L., Díaz, R. D. L., Baptista, P. M., Fernández, M. A., et al. (2019) · Scientific Reports · doi:10.1038/s41598-019-40042-6 · Cited by 12 · see citing works
Nowacka, A., Śniegocki, M., Śniegocka, M., & Ziółkowska, E. (2025) · Antioxidants · doi:10.3390/antiox14080911 · Cited by 11 · see citing works
“PLP’s ability to chelate aldehydes and act as a carbonyl scavenger also supports its antioxidant function, particularly under conditions of muscle inflammation and metabolic stress [121,122].”
The repair capacity spectrum of human skeletal muscle injury from sports to surgical trauma settings
Højfeldt, G. W., Hoegsbjerg, C., Keudell, A. G. V., & Mackey, A. L. (2025) · The Journal of Physiology · doi:10.1113/jp286507 · Cited by 10 · see citing works
“…have been seen following NMES despite circulating CK values of approximately 30,000–90,000 U/L peaking 4 days following NMES (Karlsen et al., 2020; Mackey et al., 2004, 2016), and myoglobin levels averaging 1048 μg/L on day 2 post NMES, with some individuals exceeding the assay upper detection…”
InfluentialANTI-INFLAMMATORIES FOR DELAYED ONSET MUSCLE SORENESS: SYSTEMATIC REVIEW AND META-ANALYSIS
Nahon, R. L., Lopes, J. S. S., Neto, A. M. D. M., Machado, A. D. S., & Cameron, L. C. (2021) · Revista Brasileira de Medicina do Esporte · doi:10.1590/1517-8692202127062021_0072 · Cited by 9 · see citing works
The Influence and Delivery of Cytokines and their Mediating Effect on Muscle Satellite Cells
Nederveen, J. P., Joanisse, S., Snijders, T., & Parise, G. (2017) · Current Stem Cell Reports · doi:10.1007/s40778-017-0089-1 · Cited by 9 · see citing works
Bechshøft, C. J. L., Schjerling, P., Kjær, M., & Mackey, A. L. (2019) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00215.2019 · Cited by 9 · see citing works
“However, the slower expansion of fibroblasts is in keeping with a slower rise in gene expression of several collagen types, measured in biopsies of human skeletal muscle after damaging type exercise (17, 23, 26).”
BackgroundTime-of-day effects on ex vivo muscle contractility following short-term satellite cell ablation
Kahn, R. E., Lieber, R. L., Meza, G., Dinnunhan, F., Lacham‐Kaplan, O., Dayanidhi, S., & Hawley, J. A. (2024) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00157.2024 · Cited by 7 · see citing works
Genomic stability and telomere regulation in skeletal muscle tissue
Trajano, L. A. D. S. N., Trajano, E. T. L., Silva, M. A. D. S., Stumbo, A. C., Mencalha, A. L., & da Fonseca, A. D. S. (2018) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2018.01.004 · Cited by 7 · see citing works
Platelet Rich Plasma: Postprocedural Considerations for the Sports Medicine Professional
Kolber, M. J., Purita, J. R., Paulus, C., Carreno, J. A., & Hanney, W. J. (2018) · Strength and conditioning journal · doi:10.1519/ssc.0000000000000403 · Cited by 7 · see citing works
“In the study, NSAID consumption was associated with increased satellite cell activation compared with placebo, suggesting that NSAIDs may have a different effect on injured versus healthy muscle (60).”
BackgroundRoberts, B. M., Geddis, A. V., Ciuciu, A., Reynoso, M., Mehta, N. K., Varanoske, A. N., Kelley, A. M., Walker, R., Munoz, R., Kolb, A. L., et al. (2024) · The FASEB Journal · doi:10.1096/fj.202302642r · Cited by 6 · see citing works
“79 To better understand the high-concentration effects of ACE in skeletal muscle, we used a human muscle cell culture model similar to previous experiments.”
MethodRoberts, B. M., Geddis, A. V., Sczuroski, C. E., Reynoso, M., Hughes, J. M., Gwin, J. A., & Staab, J. S. (2024) · European Journal of Applied Physiology · doi:10.1007/s00421-024-05565-5 · Cited by 5 · see citing works
“Recently, Mackey et al. found that ibuprofen accelerated satellite cell activation after electrically induced muscle injury (Mackey et al. 2016).”
Effect of Ibuprofen on Skeletal Muscle of Dysferlin-Null Mice
Collier, A. F., Gumerson, J., Lehtimäki, K. K., Puoliväli, J. T., Jones, J. W., Kane, M. A., Manne, S., O’Neill, A., Windish, H. P., Ahtoniemi, T., et al. (2017) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1124/jpet.117.244244 · Cited by 5 · see citing works
“***Indicate P , 0.001 for differences between C57BL/6 and BlAJ, with or without ibuprofen treatment. at A SPE T Journals on D ecem ber 1, 2022 jpet.aspetjournals.org D ow nloaded from also Ho et al., 2017; but see Krentz et al., 2008; Mackey et al., 2016).”
BackgroundInfluentialEvidenz von Trainingsempfehlungen für ein Hypertrophietraining
Gavanda, S., & Isenmann, E. (2021) · B&G Bewegungstherapie und Gesundheitssport · doi:10.1055/a-1382-2776 · Cited by 4 · see citing works
(2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1805313115 · Cited by 4 · see citing works
Eccentric Overload during Resistance Exercise: A Stimulus for Enhanced Satellite Cell Activation
WEHRSTEIN, M., SCHÖFFEL, A., WEIBERG, N., GWECHENBERGER, T., BETZ, T., RITTWEG, M., Parstorfer, M., Pilz, M., & FRIEDMANN-BETTE, B. (2021) · Medicine & Science in Sports & Exercise · doi:10.1249/mss.0000000000002818 · Cited by 3 · see citing works
“However, after 200 electrically stimulated eccentric contractions, proliferating SC (Ki-67/ Pax7) peaked on day 7 (168 h) after exercise (30).”
BackgroundIntegrated Role of Nutrition and Physical Activity for Lifelong Health
Koehler, K., & Drenowatz, C. (2019) · MDPI eBooks · doi:10.3390/books978-3-03921-212-5 · Cited by 3 · see citing works
Ibuprofen and hypogonadism — bench to bedside to misinterpreted hype?
Nangia, A. K., & Jensen, D. (2018) · Nature Reviews Urology · doi:10.1038/nrurol.2018.26 · Cited by 3 · see citing works
Clarifying the link between ibuprofen and hypogonadism
Jégou, B., & Kristensen, D. M. (2019) · Nature Reviews Urology · doi:10.1038/s41585-019-0146-x · Cited by 3 · see citing works
Παπανικολάου, Κ., Draganidis, D., Chatzinikolaou, A., Laschou, V. C., Georgakouli, K., Tsimeas, P. D., Batrakoulis, A., Deli, C. K., Jamurtas, A. Z., & Fatouros, I. G. (2019) · Trials · doi:10.1186/s13063-019-3557-3 · Cited by 3 · see citing works
Højfeldt, G. W., Michaud, J., Damgaard, A., Karlog, K., Migliavacca, E., Karaz, S., Pazirandeh-Micol, E., Johansen, O. E., Karagounis, L. G., Helge, B. W., et al. (2025) · medRxiv · doi:10.1101/2025.03.24.25323226 · Cited by 3 · see citing works
“Notably, while most other models succeed at triggering the initial phase of muscle regeneration as indicated by the expansion of Pax7 + MuSCs, a robust increase in differentiating progenies (myogenin + cells and eMyHC + fibers) has been observed primarily with this NMES protocol ( 30, 31, 33-35 ).”
Højfeldt, G. W., Michaud, J., Damgaard, A., Karlog, K., Migliavacca, E., Karaz, S., Pazirandeh-Micol, E., Johansen, O. E., Karagounis, L. G., Helge, B. W., et al. (2026) · Advanced Science · doi:10.1002/advs.202518471 · Cited by 5 · see citing works
“Sample size calculation was calculated on the primary endpoint based on a variability of 20% [ 29 ] and an effect size of 30% inferred from preclinical models [ 31 ].”
InfluentialFrailty Syndrome in the Elderly and Omega-3 Polyunsaturated Fatty Acids
Cin, P., & Büyükuslu, N. (2020) · Ankara Medical Journal · doi:10.5505/amj.2020.92260 · Cited by 2 · see citing works
Paz, C. L. D. S. L., de Sá, C. K. C., Carvas, N., & Tenório, M. C. C. (2021) · Revista Brasileira de Fisiologia do Exercício · doi:10.33233/rbfex.v19i6.4406 · Cited by 2 · see citing works
Ye, L., Yao, Y., Guo, H., & Peng, Y. (2018) · Connective Tissue Research · doi:10.1080/03008207.2018.1465054 · Cited by 1 · see citing works
Rawdon, C. L., Ingalls, C., Yang, F., Otis, J. S., Brandenberger, K. J., & Jackson, M. (2024) · Annals of Applied Sport Science · doi:10.61186/aassjournal.1399
Karami, E., Bazgir, B., Shirvani, H., Mohammadi, M. T., & Khaledi, M. (2024) · Journal of Muscle Research and Cell Motility · doi:10.1007/s10974-024-09683-7 · Cited by 1 · see citing works
“A study by Mackey et al. revealed that ingestion of 1200 mg/day ibuprofen augmented the propor - tion of ActiveNotch1( + ) SCs, increased SCs content, and expedited muscle repair compared to placebo (Mackey et al. 2016 ).”
Lu, X., Rehman, H., Sercu, A. S., & Markworth, J. F. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.04.21.719989 · Cited by 1 · see citing works
Muskelverletzungen — von ultrastrukturell bis strukturell
Bloch, W. (2017) · Orthopädie & Rheuma · doi:10.1007/s15002-017-1248-6
“Für Ibuprofen konnte kürzlich ein positiver Eekt auf die Satellitenzellen nachgewiesen werden, der sich 48–96 Stunden nach der Verletzung zeigt [12].”
BackgroundO’Leary, M. F. (2018) · University of Birmingham Institutional Research Archive (University of Birmingham)
The Conservative Treatment of Muscle Injuries: General Principles
Volpi, P., & Bisciotti, G. N. (2019) · Springer eBooks · doi:10.1007/978-3-030-16158-3_7
Helsel, P. J. (2020) · VTechWorks (Virginia Tech)
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Nahon, R. L., Lopes, J. S. S., Neto, A. M., Machado, A. D. S., & Cameron, L. C. (2024) · doi:10.22541/au.170664615.56497650/v1
Zhang, X., Fan, X., Chen, Y., & Liu, Z. (2024) · Industry science and engineering. · doi:10.62381/i245907
Effects of Anti-Inflammatory and Analgesic Drugs on Recovery and Performance in Sports
Howatson, G., & Lundberg, T. R. (2025) · Springer eBooks · doi:10.1007/978-3-031-44270-4_18
A semi-automated pipeline for morphological analysis of myonuclei along single muscle fibers
Schroeder, E. T., Megowan, H. G., Luu, M., Shuaib, A., Fries, A. C., Searcy, J., & Dreyer, H. C. (2026) · iScience · doi:10.1016/j.isci.2026.116961
Højfeldt, G. W., Sorenson, T., Gonzales, A., Kjaer, M., Andersen, J. L., & Mackey, A. L. (2024) · Figshare · doi:10.6084/m9.figshare.26610135
da Fonseca, A. M., de Almeida, R. A. C. D. S., Olivares, E. L., & da Silveira, A. L. B. (2026) · Journal of Muscle Research and Cell Motility · doi:10.1007/s10974-026-09724-3
“…it is reasonable also to hypothesize that the muscle hypertrophy observed in COMB group in the present study is analogous to the findings of Mackey et al. ( 2016 ), who demonstrated that ibuprofen administration in humans, although not COX-selective but capable of inhibit - ing COX-2…”
Yang, Y., Cao, X., Li, Y., Kang, X., Huang, Y., Feng, H., & Li, C. (2026) · Immunological Investigations · doi:10.1080/08820139.2026.2667884
“…anti-inflammatories is controversial; while Ibuprofen (1200 mg/day) increased ActiveNotch1+ satellite cells by 53% at 2 days post-injury (Mackey et al., 2016), premature administration of NSAIDs may actually delay structural repair by disrupting the initial macrophage polarization…”
Jerger, S., Nielsen, J. L., Centner, C., Mathiesen, J., Suetta, C., Oesser, S., Gollhofer, A., Aagaard, P., & König, D. (2026) · Frontiers in Physiology · doi:10.3389/fphys.2026.1839695
“…myo fi ber injury resulted in a four-fold increase in the number of fi broblasts in human VL muscle, with fi broblasts preferentially localized in the proximity of regenerating muscle fi bers (Mackey et al., 2017) while accompanied by a 2-fold increase in collagen gene expression (Mackey et al., 2016).”
Downhill running differentially reduces stimulated contraction torque in human quadriceps muscles
Rawdon, C. L., Ingalls, C. P., Yang, F., Otis, J. S., Brandenberger, K. J., Jackson, M., & Middleton, R. (2026) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00765.2025
Παπανικολάου, Κ., Jamurtas, A. Z., Draganidis, D., Baloyiannis, I., Poulios, A., Rosvoglou, A., Papadopoulos, C., Papadimas, G., Chrysanthou‐Piterou, M., Havaki, S., et al. (2026) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2026.10.010
- Authors
- Mackey, A. L., Rasmussen, L. K., Kadi, F., Schjerling, P., Helmark, I. C., Ponsot, E., Aagaard, P., Durigan, J. L. Q., & Kjaer, M.
- Affiliations
- University of Copenhagen (DK)
- Bispebjerg Hospital (DK)
- Örebro University (SE)
- University of Southern Denmark (DK)
- Physical Therapy Division University of Brasília Brasília Brazil
- Institute of Sports Medicine Copenhagen Department of Orthopaedic Surgery M Bispebjerg Hospital Copenhagen Denmark
- Corresponding author
- Mackey, Abigail L.
- Venue
- The FASEB Journal
- Publisher
- Wiley
- Published
- 2016-03-02
- Volume
- 30
- Issue
- 6
- Pages
- 2266-2281
- ISSN
- 0892-6638 (ISSN-L)
- 1530-6860
- PMC
- PMC4871801
- OpenAlex
- W2295397642
- Cited by
- 99
- References
- 35
- Citations by year
- 2026: 8 · 2025: 7 · 2024: 11 · 2023: 6 · 2022: 6 · 2021: 8 · 2020: 11 · 2019: 13 · 2018: 16 · 2017: 9 · 2016: 3 · 2014: 1
- Type
- book review
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- The FASEB Journal (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Research at the University of Copenhagen (University of Copenhagen) (submittedVersion)
- University of Southern Denmark Research Portal (University of Southern Denmark) (submittedVersion)
- Funders
- Nordea-fonden
- European Commission
- Gigtforeningen
- Medical Research Council
- Lundbeckfonden
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Keywords
- nonsteroidal anti-inflammatory drugs, ibuprofen, satellite cells, skeletal muscle regeneration, Notch signaling, Pax7, muscle damage, electrical stimulation, telomere length, muscle remodeling, vastus lateralis
- MeSH terms
- Adolescent, Anti-Inflammatory Agents, Non-Steroidal, Biopsy, Double-Blind Method, Electric Stimulation, Gene Expression Regulation, Humans, Ibuprofen, Male, Regeneration, RNA, Muscle, Skeletal, Satellite Cells, Skeletal Muscle, Receptor, Notch1, Young Adult
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