Cited by 511Muscle Physiology and DisordersDevelopmental Biology and Gene RegulationCongenital heart defects research

A Subpopulation of Adult Skeletal Muscle Stem Cells Retains All Template DNA Strands after Cell Division

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Rocheteau, Pierre, Gayraud-Morel, Barbara, Siegl-Cachedenier, Irene, Blasco, Maria A., Tajbakhsh, Shahragim·2012·Cell

Abstract

Satellite cells are adult skeletal muscle stem cells that are quiescent and constitute a poorly defined heterogeneous population. Using transgenic Tg:Pax7-nGFP mice, we show that Pax7-nGFP(Hi) cells are less primed for commitment and have a lower metabolic status and delayed first mitosis compared to Pax7-nGFP(Lo) cells. Pax7-nGFP(Hi) can give rise to Pax7-nGFP(Lo) cells after serial transplantations. Proliferating Pax7-nGFP(Hi) cells exhibit lower metabolic activity, and the majority performs asymmetric DNA segregation during cell division, wherein daughter cells retaining template DNA strands express stem cell markers. Using chromosome orientation-fluorescence in situ hybridization, we demonstrate that all chromatids segregate asymmetrically, whereas Pax7-nGFP(Lo) cells perform random DNA segregation. Therefore, quiescent Pax7-nGFP(Hi) cells represent a reversible dormant stem cell state, and during muscle regeneration, Pax7-nGFP(Hi) cells generate distinct daughter cell fates by asymmetrically segregating template DNA strands to the stem cell. These findings provide major insights into the biology of stem cells that segregate DNA asymmetrically.

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Crossref · 10.1016/j.cell.2011.11.049

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57 of 57 references verified0 citation errors · 0 not checkable

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  • Asymmetric chromosome segregation occurs in MuSCs, ISCs, and NSCs.

    “There is mounting evidence of asymmetric chromosome segregation in stem cells such as MuSCs17,18,129, ISCs20,130,131 and NSCs132.”

    From Molecular regulation of stem cell quiescence (2013) · cites this paper as [129]

  • In muscle, MuSCs exist as a heterogeneous quiescent population in adult mice, with changes in heterogeneity with ageing.

    “In muscle, MuSCs also exist as a heterogeneous quiescent population in adult mice30–33, with changes in heterogeneity with ageing32,34,35.”

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Authors
Rocheteau, P., Gayraud-Morel, B., Siegl-Cachedenier, I., Blasco, M. A., & Tajbakhsh, S.
Affiliations
Centre National de la Recherche Scientifique (FR)
Corresponding author
Tajbakhsh, Shahragim
Venue
Cell
Publisher
Elsevier BV
Published
2012-01
Volume
148
Issue
1-2
Pages
112-125
ISSN
0092-8674 (ISSN-L)
OpenAlex
W2037334278
Cited by
510
References
57
Citations by year
2026: 16 · 2025: 17 · 2024: 34 · 2023: 45 · 2022: 33 · 2021: 29 · 2020: 31 · 2019: 33 · 2018: 51 · 2017: 41 · 2016: 30 · 2015: 38 · 2014: 39 · 2013: 40 · 2012: 31
FWCI
19.04
Type
journal article
Language
en
Open access
Locations
  • Cell (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Agence Nationale de la Recherche (ANR-06-BLAN-0039)
  • Centre National de la Recherche Scientifique
  • Ministerio de Ciencia e Innovación
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Congenital heart defects research
Keywords
skeletal muscle stem cells, satellite cells, Pax7, stem cell quiescence, cell fate determination, muscle regeneration, cellular metabolism, first mitosis, stem cell heterogeneity, serial transplantation
MeSH terms
Animals, Cell Division, Female, Mice, Transgenic, Templates, Genetic, Muscle, Skeletal, Chromosome Segregation, Satellite Cells, Skeletal Muscle, Mice, PAX7 Transcription Factor, Adult Stem Cells

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