Cited by 114Bacterial Genetics and BiotechnologyProbiotics and Fermented FoodsMicrobial Metabolic Engineering and Bioproduction

Relaxed control of sugar utilization in Lactobacillus brevis

Kim, Jae-Han, Shoemaker, Sharon P., Mills, David A.·2009·Microbiology

Abstract

Prioritization of sugar consumption is a common theme in bacterial growth and a problem for complete utilization of five and six carbon sugars derived from lignocellulose. Growth studies show that Lactobacillus brevis simultaneously consumes numerous carbon sources and appears to lack normal hierarchical control of carbohydrate utilization. Analysis of several independent L. brevis isolates indicated that co-utilization of xylose and glucose is a common trait for this species. Moreover, carbohydrates that can be used as a single carbon source are simultaneously utilized with glucose. Analysis of the proteome of L. brevis cells grown on glucose, xylose or a glucose/xylose mixture revealed the constitutive expression of the enzymes of the heterofermentative pathway. In addition, fermentative mass balances between mixed sugar inputs and end-products indicated that both glucose and xylose are simultaneously metabolized through the heterofermentative pathway. Proteomic and mRNA analyses revealed that genes in the xyl operon were expressed in the cells grown on xylose or on glucose/xylose mixtures but not in those grown on glucose alone. However, the expression level of XylA and XylB proteins in cells grown on a glucose/xylose mixture was reduced 2.7-fold from that observed in cells grown solely on xylose. These results suggest that regulation of xylose utilization in L. brevis is not stringently controlled as seen in other lactic acid bacteria, where carbon catabolite repression operates to prioritize carbohydrate utilization more rigorously.

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Crossref · 10.1099/mic.0.024653-0

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Authors
Kim, J.-H., Shoemaker, S. P., & Mills, D. A.
Affiliations
University of California, Davis (US)
Venue
Microbiology
Published
2009-04-01
Volume
155
Issue
4
Pages
1351-1359
ISSN
  • 1350-0872 (ISSN-L)
  • 1465-2080
OpenAlex
W2149041816
Cited by
114
References
42
Citations by year
2026: 1 · 2025: 6 · 2024: 4 · 2023: 9 · 2022: 5 · 2021: 6 · 2020: 9 · 2019: 9 · 2018: 5 · 2017: 10 · 2016: 6 · 2015: 5 · 2014: 8 · 2013: 10 · 2012: 6
FWCI
4.07
Type
journal article
Language
en
Open access
closed
Locations
  • Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
U.S. Department of Energy
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Keywords
Lactobacillus brevis, sugar utilization, xylose metabolism, glucose-xylose co-utilization, xyl operon, catabolite repression, proteome, gene expression
MeSH terms
Bacterial Proteins, Culture Media, Glucose, Operon, RNA, Messenger, Xylose, Gene Expression Regulation, Bacterial, Proteomics, Levilactobacillus brevis

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