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PreprintCited by 15DNA Repair MechanismsMicrotubule and mitosis dynamicsEffects of Radiation Exposure

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Microtubule-associated proteins MAP7 and MAP7D1 promote DNA double strand break repair in the G1 cell cycle phase

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Dullovi, Arlinda, Ozgencil, Meryem, Rajvee, Vinothini, Tse, Wai Yiu, Cutillas, Pedro R, Martin, Sarah A, Hořejší, Zuzana·2023·iScience
DOI 10.1101/2023.01.17.524354PMC PMC9958362

Preprint — published as 10.1016/j.isci.2023.106107 · Paper page

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Abstract

Summary: The DNA-damage response is a complex signalling network that guards genomic integrity. The microtubule cytoskeleton is involved in the repair of DNA double-strand breaks; however, little is known about which cytoskeleton-related proteins are involved in DNA repair and how. Using quantitative proteomics, we discovered that microtubule associated proteins MAP7 and MAP7D1 interact with several DNA repair proteins including DNA double-strand break repair proteins RAD50, BRCA1 and 53BP1. We observed that downregulation of MAP7 and MAP7D1 leads to increased phosphorylation of p53 after γ- irradiation. Moreover, we determined that the downregulation of MAP7D1 leads to a strong G1 arrest and that the downregulation of MAP7 and MAP7D1 in cells arrested in G1 negatively affects DNA repair, recruitment of RAD50 to chromatin and localisation of 53BP1 to the sites of damage. These findings describe for the first time a novel function of MAP7 and MAP7D1 in cell cycle regulation and cellular response to DNA double-strand breaks.

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Metadata

Authors
Dullovi, A., Ozgencil, M., Rajvee, V., Tse, W. Y., Cutillas, P. R., Martin, S. A., & Hořejší, Z.
Affiliations
Queen Mary University of London (GB)
ORCID
Venue
iScience
Publisher
openRxiv
Published
2023-01-17
Volume
26
Issue
3
Pages
106107-106107
ISSN
2589-0042 (ISSN-L)
DOI
10.1101/2023.01.17.524354
PMC
PMC9958362
OpenAlex
W4318773798
Cited by
15
References
1
Citations by year
2026: 4 · 2025: 5 · 2024: 4 · 2023: 2
FWCI
1.33
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • iScience (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Queen Mary University of London
  • Wellcome Trust (200462/Z/16/Z)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Effects of Radiation Exposure
Keywords
MAP7, microtubule-associated proteins, DNA double-strand break repair, G1 cell cycle arrest, DNA damage response, RAD50, BRCA1, 53BP1, p53 phosphorylation, gamma irradiation, microtubule cytoskeleton, chromatin recruitment

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