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Cited by 93Microtubule and mitosis dynamicsCellular transport and secretionGenetic and Kidney Cyst Diseases

Concerted action of kinesins KIF5B and KIF13B promotes efficient secretory vesicle transport to microtubule plus ends

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Serra-Marques, Andrea, Martin, Maud, Katrukha, Eugene A, Grigoriev, Ilya, Peeters, Cathelijn AE, Liu, Qingyang, Hooikaas, Peter Jan, Yao, Yao +6 more·2020·eLife
DOI 10.7554/elife.61302PMC PMC7710357

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Abstract

Intracellular transport relies on multiple kinesins, but it is poorly understood which kinesins are present on particular cargos, what their contributions are and whether they act simultaneously on the same cargo. Here, we show that Rab6-positive secretory vesicles are transported from the Golgi apparatus to the cell periphery by kinesin-1 KIF5B and kinesin-3 KIF13B, which determine the location of secretion events. KIF5B plays a dominant role, whereas KIF13B helps Rab6 vesicles to reach freshly polymerized microtubule ends, to which KIF5B binds poorly, likely because its cofactors, MAP7-family proteins, are slow in populating these ends. Sub-pixel localization demonstrated that during microtubule plus-end directed transport, both kinesins localize to the vesicle front and can be engaged on the same vesicle. When vesicles reverse direction, KIF13B relocates to the middle of the vesicle, while KIF5B shifts to the back, suggesting that KIF5B but not KIF13B undergoes a tug-of-war with a minus-end directed motor.

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  • Distribution of mitochondria throughout the cell is Kinesin-1-dependent, and absence of Kinesin-1 or its activating MAP, MAP7, leads to clustering of mitochondria around the nucleus.

    “Distribution of mitochondria throughout the cell is Kinesin-1–dependent and the absence of Kinesin-1 or its activating MAP, MAP7, leads to the clustering of mitochondria around the nucleus.”

    From A live-cell marker to visualize the dynamics of stable microtubules throughout the cell cycle (2023) · cites this paper as Serra-Marques et al., 2020

    The Results section ('KIF5B and KIF13B are the main drivers of Rab6 vesicle transport in HeLa cells'; page unavailable) reports that mitochondria shifted toward the perinuclear region in cell lines lacking KIF5B. The Discussion says MAP7 family members recruit and activate KIF5B, but our copy does not show that loss of MAP7 causes mitochondrial clustering. (LLM, conf=0.87)

    Experimental — accuracy not yet measured

  • The presence of the N-terminal FKBP domain in the Rab6a construct has no detectable effects on the behavior of this marker.

    “The presence of the N-terminal FKBP domain in the Rab6a construct has no detectable effects on the behavior of this marker (Schlager et al., 2014; Serra-Marques et al., 2020).”

    From A live-cell marker to visualize the dynamics of stable microtubules throughout the cell cycle (2023) · cites this paper as Serra-Marques et al., 2020

  • Rab6a secretory vesicles are transported from the Golgi to the plasma membrane by the concerted action of Kif5b (Kinesin-1) and Kif13b (Kinesin-3).

    “Rab6a secretory vesicles are transported from the Golgi to the plasma membrane by the concerted action of Kif5b (Kinesin-1) and Kif13b (Kinesin-3; Serra-Marques et al., 2020).”

    From A live-cell marker to visualize the dynamics of stable microtubules throughout the cell cycle (2023) · cites this paper as Serra-Marques et al., 2020

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Authors
Serra-Marques, A., Martin, M., Katrukha, E. A., Grigoriev, I., Peeters, C. A., Liu, Q., Hooikaas, P. J., Yao, Y., Solianova, V., Smal, I., Pedersen, L. B., Meijering, E., Kapitein, L. C., & Akhmanova, A.
Affiliations
  • Utrecht University (NL)
  • Erasmus MC (NL)
  • University of Copenhagen (DK)
Corresponding author
Akhmanova, Anna
ORCID
Venue
eLife
Publisher
eLife Sciences Publications, Ltd
Published
2020-11-11
Volume
9
ISSN
2050-084X (ISSN-L)
DOI
10.7554/elife.61302
PMC
PMC7710357
OpenAlex
W3102863060
Cited by
93
References
3
Citations by year
2026: 12 · 2025: 10 · 2024: 28 · 2023: 15 · 2022: 17 · 2021: 11
FWCI
5.36
Type
journal article
Language
en
Open access
Licence
Locations
  • eLife (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Research at the University of Copenhagen (University of Copenhagen) (publishedVersion, cc-by)
  • EUR Research Repository (Erasmus University Rotterdam) (publishedVersion, other-oa)
  • EUR Research Repository (Erasmus University Rotterdam) (submittedVersion, other-oa)
  • RePub (Erasmus University Rotterdam) (submittedVersion, other-oa)
  • Dépôt institutionnel de l'Université libre de Bruxelles (Université Libre de Bruxelles) (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Utrecht University Repository (Utrecht University) (submittedVersion, other-oa)
Funders
  • H2020 European Research Council (Synergy grant 609822, Consolidator grant 819219)
  • Nederlandse Organisatie voor Wetenschappelijk Onderzoek (ALW Open Program grant 824.15.017, STW grant OTP13391)
  • H2020 Marie Skłodowska-Curie Actions (IEF fellowship)
  • Fundação para a Ciência e a Tecnologia (PhD fellowship)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
Keywords
KIF5B, KIF13B, kinesin-1, kinesin-3, secretory vesicle transport, Golgi apparatus, microtubule plus ends, intracellular transport, sub-pixel localization
MeSH terms
HeLa Cells, Humans, Microtubules, Kinesins, rab GTP-Binding Proteins, Protein Transport, Transport Vesicles

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