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PreprintCited by 30Cellular transport and secretionUbiquitin and proteasome pathwaysLipid Membrane Structure and Behavior

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Evolutionarily conserved principles of ESCRT-III-mediated membrane remodelling revealed by a two-subunit Asgard archaeal system

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Souza, Diorge P., Espadas, Javier, Chaaban, Sami, Moody, Edmund R. R., Hatano, Tomoyuki, Balasubramanian, Mohan, Williams, Tom A., Roux, Aurélien, Baum, Buzz·2024·Science Advances
DOI 10.1101/2024.07.01.601590PMC PMC11804906

Preprint — published as 10.1126/sciadv.ads5255 · Paper page

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Abstract

Summary: ESCRT-III proteins assemble into composite polymers that undergo stepwise changes in composition and structure to deform membranes across the tree of life. Here, using a phylogenetic analysis we demonstrate that the two ESCRT-III proteins present in our closest archaeal relatives are evolutionarily related to B-type and A-type eukaryotic paralogues, which initiate and execute membrane remodelling, respectively. This deep homology is reflected in ESCRT-III structure and function as demonstrated by the fact that ESCRT-IIIB assembles into parallel arrays on planar membranes to initiate membrane deformation, and is required to recruit ESCRT-IIIA to generate composite polymers. ESCRT-IIIA homopolymers can then remodel membranes into tubes, as a likely prelude to scission. Taken together, this analysis reveals a set of conserved principles governing ESCRT-III-dependent membrane remodelling that first emerged with the evolution of a two-component ESCRT-III system in the Asgard archaea, and which continue to underlie complex multi-component, ESCRT-III-dependent membrane remodelling in eukaryotes.

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Metadata

Authors
Souza, D. P., Espadas, J., Chaaban, S., Moody, E. R. R., Hatano, T., Balasubramanian, M., Williams, T. A., Roux, A., & Baum, B.
Affiliations
  • University of Geneva (CH)
  • MRC Laboratory of Molecular Biology (GB)
  • University of Bristol (GB)
  • University of Warwick (GB)
Corresponding author
Baum, Buzz
ORCID
Venue
Science Advances
Publisher
openRxiv
Published
2024-07-02
Volume
11
Issue
6
Pages
eads5255-eads5255
ISSN
2375-2548 (ISSN-L)
DOI
10.1101/2024.07.01.601590
PMC
PMC11804906
OpenAlex
W4407279138
Cited by
30
References
1
Citations by year
2026: 14 · 2025: 14 · 2024: 2
FWCI
13.98
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Science Advances (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Bristol Research (University of Bristol) (publishedVersion, cc-by)
  • Warwick Research Archive Portal (University of Warwick) (acceptedVersion)
  • Archive ouverte UNIGE (University of Geneva) (submittedVersion, cc-by)
  • Explore Bristol Research (submittedVersion, cc-by)
Funders
  • Wellcome Trust (210711/Z/18/Z, 203276/Z/16/Z)
  • Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (200793, 189996, 310030)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ubiquitin and proteasome pathways
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
Keywords
ESCRT-III, membrane remodeling, Asgard archaea, ESCRT, membrane deformation, membrane fission, phylogenetic analysis
MeSH terms
Archaea, Cell Membrane, Phylogeny, Evolution, Molecular, Archaeal Proteins, Endosomal Sorting Complexes Required for Transport

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