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Looking for Therapeutic Antibodies in Next Generation Sequencing Repositories
Open accessAbstract
Recently it has become possible to query the great diversity of natural antibody repertoires using Next Generation Sequencing (NGS). These methods are capable of producing millions of sequences in a single experiment. Here we compare Clinical Stage Therapeutic antibodies to the ∼1b sequences from 60 independent sequencing studies in the Observed Antibody Space Database. Of the 242 post Phase I antibodies, we find 16 with sequence identity matches of 95% or better for both heavy and light chains. There are also 54 perfect matches to therapeutic CDR-H3 regions in the NGS outputs, suggesting a nontrivial amount of convergence between naturally observed sequences and those developed artificially. This has potential implications for both the discovery of antibody therapeutics and the legal protection of commercial antibodies.
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“Furthermore, many therapeutic CDRH3 loops can be found in naturally sourced NGS datasets, indicating a certain degree of convergence between antibodies raised experimentally and naturally occurring ones [225].”
From Computational approaches to therapeutic antibody design: established methods and emerging trends (2020) · cites this paper as [225]
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Metadata
- Authors
- Krawczyk, K., Raybould, M., Kovaltsuk, A., & Deane, C. M.
- Affiliations
- University of Oxford (GB)
- Corresponding author
- Krawczyk, Konrad
- Venue
- mAbs
- Publisher
- openRxiv
- Published
- 2019-03-09
- Volume
- 11
- Issue
- 7
- Pages
- 1197-1205
- ISSN
- 1942-0862 (ISSN-L)
- PMC
- PMC6748601
- OpenAlex
- W2950135969
- Cited by
- 29
- References
- 40
- Citations by year
- 2025: 3 · 2024: 2 · 2023: 3 · 2022: 9 · 2021: 5 · 2020: 5 · 2019: 2
- FWCI
- 3.47
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- mAbs (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Engineering and Physical Sciences Research Council (1795796)
- Biotechnology and Biological Sciences Research Council (1801486, BB/M011224/1)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Monoclonal and Polyclonal Antibodies Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › vaccines and immunoinformatics approaches
- Life Sciences › Immunology and Microbiology › Immunology › Biosimilars and Bioanalytical Methods
- Keywords
- therapeutic antibodies, immunoglobulin gene repertoire
- MeSH terms
- Humans, Immunoglobulins, Immunotherapy, Complementarity Determining Regions, Databases, Genetic, Immunity, Humoral, Data Mining, High-Throughput Nucleotide Sequencing
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