The DOI matches its Crossref record.
Expression of a dominant-negative Wnt blocks induction of MyoD in Xenopus embryos.
Open accessAbstract
During gastrulation of Xenopus embryos the prospective mesoderm is induced initially with domains of dorsal and ventral fate, then further patterned to generate somitic mesoderm by signals from the gastrula organizer. Although Xwnt-8 is expressed in future ventrolateral mesoderm and induces prospective epidermis to differentiate in vitro as ventral mesoderm, no loss-of-function studies have demonstrated a requirement for Wnt signaling for the normal expression of mesodermal genes in the gastrula. We report development of a dominant-negative Wnt (dnXwnt-8) that inhibits embryonic responses to Wnt signaling in a cell-nonautonomous fashion. By expressing dnXwnt-8 in Xenopus embryos, we uncover a requirement of Wnt signaling for localized expression in prospective mesoderm of XMyoDa and Xenopus-posterior (Xpo). Because ectopic expression of functional Xwnt-8 in the dorsal marginal zone of the gastrula induces ectopic XMyoDa and Xpo, both gain-of-function and loss-of-function experiments support a model in which endogenous Xwnt-8 functions to induce expression of genes involved in specification of ventral and somitic mesoderm.
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Cited by
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Signal transduction through beta-catenin and specification of cell fate during embryogenesis.
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Kühl, M., Sheldahl, L. C., Malbon, C. C., & Moon, R. T. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.17.12701 · Cited by 517
Self-citationCasein kinase I transduces Wnt signals
Peters, J. M., McKay, R. M., McKay, J. P., & Graff, J. M. (1999) · Nature · doi:10.1038/43830 · Cited by 447
The establishment of spemann's organizer and patterning of the vertebrate embryo
De Robertis, E. M., Larraín, J., Oelgeschläger, M., & Wessely, O. (2000) · Nature Reviews Genetics · doi:10.1038/35042039 · Cited by 429
R-Spondin2 Is a Secreted Activator of Wnt/β-Catenin Signaling and Is Required for Xenopus Myogenesis
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Wnt10b Deficiency Promotes Coexpression of Myogenic and Adipogenic Programs in Myoblasts
Vertino, A. M., Taylor-Jones, J. M., Longo, K. A., Bearden, E. D., Lane, T. F., McGehee, R. E., MacDougald, O. A., & Peterson, C. A. (2005) · Molecular Biology of the Cell · doi:10.1091/mbc.e04-08-0720 · Cited by 158
A Small‐Molecule Agonist of the Wnt Signaling Pathway
Liu, J., Wu, X., Mitchell, B., Kintner, C., Ding, S., & Schultz, P. G. (2005) · Angewandte Chemie International Edition · doi:10.1002/anie.200462552 · Cited by 142
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Metadata
- Authors
- Hoppler, S., Brown, J. D., & Moon, R. T.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 1996-11-01
- Volume
- 10
- Issue
- 21
- Pages
- 2805-2817
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2027255572
- Cited by
- 342
- References
- 0
- Type
- posted content
- Language
- en
- Open access
- diamond
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