Conversion of Dorsal from an activator to a repressor by the global corepressor Groucho
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The Dorsal morphogen acts as both an activator and a repressor of transcription in the Drosophila embryo to regulate the expression of dorsal/ventral patterning genes. Circumstantial evidence has suggested that Dorsal is an intrinsic activator and that additional factors (corepressors) convert it into a repressor. These corepressors, however, have previously eluded definitive identification. We show here, via the analysis of embryos lacking the maternally encoded Groucho corepressor and via protein-binding assays, that recruitment of Groucho to the template by protein:protein interactions is required for the conversion of Dorsal from an activator to a repressor. Groucho is therefore a critical component of the dorsal/ventral patterning system.
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Dorsal functions as both an activator of transcription to induce gene expression and a repressor to keep genes silenced.
“Dorsal functions as both an activator of transcription to induce gene expression and a repressor to keep genes silenced”
From Graded dorsal and differential gene regulation in the Drosophila embryo. (2009) · cites this paper as Dubnicoff et al. 1997
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Self-citationUncoupling Dorsal-mediated activation from Dorsal-mediated repression in the<i>Drosophila</i>embryo
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Hunchback is counter-repressed to regulate even-skipped stripe 2 expression in Drosophila embryos
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Receptor tyrosine kinase signaling regulates different modes of Groucho-dependent control of Dorsal
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- Authors
- Dubnicoff, T., Valentine, S. A., Chen, G., Shi, T., Lengyel, J. A., Paroush, Z., & Courey, A. J.
- Affiliations
- University of California, Los Angeles (US)
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 1997-11-15
- Volume
- 11
- Issue
- 22
- Pages
- 2952-2957
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- PMC
- PMC316698
- OpenAlex
- W2033496245
- Cited by
- 155
- References
- 39
- Citations by year
- 2025: 1 · 2024: 5 · 2023: 1 · 2022: 1 · 2021: 3 · 2020: 2 · 2019: 2 · 2018: 5 · 2017: 3 · 2016: 4 · 2015: 3 · 2014: 3 · 2013: 3 · 2012: 5
- Type
- posted content
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Genes & Development (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HighWire Press Open Archive (submittedVersion)
- Funders
- Israel Cancer Research Fund
- Israel Academy of Sciences and Humanities
- National Institutes of Health (5r01gm051270-04)
- National Institute of General Medical Sciences (GM51270, GM44522, R01GM044522)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
- Keywords
- transcriptional repression, dorsoventral patterning, Drosophila embryo, protein-protein interaction, corepressor recruitment, maternal gene expression
- MeSH terms
- Animals, Blastoderm, DNA-Binding Proteins, Drosophila melanogaster, Nuclear Proteins, Phosphoproteins, Protein Binding, Repressor Proteins, Transcription Factors, Gene Expression Regulation, Developmental, Drosophila Proteins, Basic Helix-Loop-Helix Transcription Factors, Basic Helix-Loop-Helix Proteins
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