The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Palmitoylation is required for the production of a soluble multimeric Hedgehog protein complex and long-range signaling in vertebrates
Open accessAbstract
Hedgehog (Hh) signaling plays a major role in multiple aspects of embryonic development. A key issue in Hh signaling is to elucidate the molecular mechanism by which a Hh protein morphogen gradient is formed despite its membrane association. In this study, we used a combination of genetic, cellular, and biochemical approaches to address the role of lipid modifications in long-range vertebrate Hh signaling. Our molecular analysis of knockout mice deficient in Skn , the murine homolog of the Drosophila ski gene, which catalyzes Hh palmitoylation, and gene-targeted mice producing a nonpalmitoylated form of Shh indicates that Hh palmitoylation is essential for its activity as well as the generation of a protein gradient in the developing embryos. Furthermore, our biochemical data show that Hh lipid modifications are required for producing a soluble multimeric protein complex, which constitutes the major active component for Hh signaling. These results suggest that soluble Hh multimeric complex travels in the morphogenetic field to activate Hh signaling in distant Hh-responsive cells.
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Cites
Wnt proteins are lipid-modified and can act as stem cell growth factors
Willert, K., Brown, J. D., Danenberg, E., Duncan, A. W., Weissman, I. L., Reya, T., Yates, J. R., & Nusse, R. (2003) · Nature · doi:10.1038/nature01611 · Cited by 2,257
Ang, S.-L., Wierda, A., Wong, D., Stevens, K. A., Cascio, S., Rossant, J., & Zaret, K. S. (1993) · Development · doi:10.1242/dev.119.4.1301 · Cited by 618 · cited 1×
“2R; Ang et al. 1993; Sasaki and Hogan 1993; data not shown) are not detected in the ventral midline of the neural tube except at the more rostral and caudal regions where residual Shh and HNF3 expression in the ventral midline could be detected (Fig.”
BackgroundA superfamily of membrane-bound O -acyltransferases with implications for Wnt signaling
Hofmann, K. (2000) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(99)01539-x · Cited by 495
Similarities between the Hedgehog and Wnt signaling pathways
Kalderon, D. (2002) · Trends in Cell Biology · doi:10.1016/s0962-8924(02)02388-7 · Cited by 141
Cited by
The mechanisms of Hedgehog signalling and its roles in development and disease
Briscoe, J., & Thérond, P. P. (2013) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3598 · Cited by 1,751
Hedgehog: functions and mechanisms
Varjosalo, M., & Taipale, J. (2008) · Genes & Development · doi:10.1101/gad.1693608 · Cited by 1,217
Dessaud, E., McMahon, A. P., & Briscoe, J. (2008) · Development · doi:10.1242/dev.009324 · Cited by 734
Functions of heparan sulfate proteoglycans in cell signaling during development
Lin, X. (2004) · Development · doi:10.1242/dev.01522 · Cited by 678
Mechanisms and functions of Hedgehog signalling across the metazoa
Ingham, P. W., Nakano, Y., & Seger, C. (2011) · Nature Reviews Genetics · doi:10.1038/nrg2984 · Cited by 605
Metadata
- Authors
- Chen, M.-H., Li, Y.-J., Kawakami, T., Xu, S.-M., & Chuang, P.-T.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2004-03-15
- Volume
- 18
- Issue
- 6
- Pages
- 641-659
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2153188090
- Cited by
- 368
- References
- 4
- Type
- posted content
- Language
- en
- Open access
- diamond
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- Chen, M.-H., Li, Y.-J., Kawakami, T., Xu, S.-M., & Chuang, P.-T. (2004). Palmitoylation is required for the production of a soluble multimeric Hedgehog protein complex and long-range signaling in vertebrates. Genes & Development. https://doi.org/10.1101/gad.1185804
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