Gravin regulates mesodermal cell behavior changes required for axis elongation during zebrafish gastrulation

Weiser, Douglas C.; Pyati, Ujwal J.; Kimelman, David

doi:10.1101/gad.1535007

Cited by 62Developmental Biology and Gene RegulationCellular Mechanics and InteractionsZebrafish Biomedical Research Applications

Gravin regulates mesodermal cell behavior changes required for axis elongation during zebrafish gastrulation

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Weiser, Douglas C., Pyati, Ujwal J., Kimelman, David·2007·Genes & Development

DOI 10.1101/gad.1535007PMC PMC1891432

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Abstract

Convergent extension of the mesoderm is the major driving force of vertebrate gastrulation. During this process, mesodermal cells move toward the future dorsal side of the embryo, then radically change behavior as they initiate extension of the body axis. How cells make this transition in behavior is unknown. We have identified the scaffolding protein and tumor suppressor Gravin as a key regulator of this process in zebrafish embryos. We show that Gravin is required for the conversion of mesodermal cells from a highly migratory behavior to the medio-laterally intercalative behavior required for body axis extension. In the absence of Gravin, paraxial mesodermal cells fail to shut down the protrusive activity mediated by the Rho/ROCK/Myosin II pathway, resulting in embryos with severe extension defects. We propose that Gravin functions as an essential scaffold for regulatory proteins that suppress the migratory behavior of the mesoderm during gastrulation, and suggest that this function also explains how Gravin inhibits invasive behaviors in metastatic cells.

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Activation of Rho GTPase leads to the activation of the Rho-associated kinase (ROCK) and myosin, which leads to modification of the actin cytoskeleton and cytoskeletal rearrangement.
“Activation of Rho GTPase leads to the activation of the Rho-associated kinase (ROCK)82 and myosin,83 which leads to modification of the actin cytoskeleton and cytoskeletal rearrangement.”
From Wnt signal transduction pathways (2008) · cites this paper as [83]

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The zebrafish book : a guide for the laboratory use of zebrafish (Danio rerio)
Westerfield, M. (1995) · Medical Entomology and Zoology · Cited by 6,813 · see citing works
Rho GTPases and the Actin Cytoskeleton
Hall, A. (1998) · Science · doi:10.1126/science.279.5350.509 · Cited by 6,138 · see citing works
Tumour-cell invasion and migration: diversity and escape mechanisms
Friedl, P., & Wolf, K. (2003) · Nature reviews. Cancer · doi:10.1038/nrc1075 · Cited by 3,225 · see citing works
Compensation mechanism in tumor cell migration
Wolf, K., Mazo, I. B., Leung, H. C. H., Engelke, K., von Andrian, U. H., Deryugina, E. I., Strongin, A. Y., Bröcker, E., & Friedl, P. (2003) · The Journal of Cell Biology · doi:10.1083/jcb.200209006 · Cited by 1,392 · see citing works
A Second Canon
Veeman, M. T., Axelrod, J. D., & Moon, R. T. (2003) · Developmental Cell · doi:10.1016/s1534-5807(03)00266-1 · Cited by 1,359 · see citing works
Differing modes of tumour cell invasion have distinct requirements for Rho/ROCK signalling and extracellular proteolysis
Sahai, E. A., & Marshall, C. J. (2003) · Nature Cell Biology · doi:10.1038/ncb1019 · Cited by 1,148 · see citing works
AKAP signalling complexes: focal points in space and time
Wong, W., & Scott, J. D. (2004) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1527 · Cited by 1,073 · see citing works
Silberblick/Wnt11 mediates convergent extension movements during zebrafish gastrulation
Heisenberg, C.-P., Tada, M., Rauch, G.-J., Saúde, L., Concha, M. L., Geisler, R., Stemple, D. L., Smith, J. C., & Wilson, S. W. (2000) · Nature · doi:10.1038/35011068 · Cited by 1,013 · see citing works
Wnt/Frizzled Activation of Rho Regulates Vertebrate Gastrulation and Requires a Novel Formin Homology Protein Daam1
Habas, R., Kato, Y., & He, X. (2001) · Cell · doi:10.1016/s0092-8674(01)00614-6 · Cited by 842 · see citing works
Dishevelled controls cell polarity during Xenopus gastrulation
Wallingford, J. B., Rowning, B. A., Vogeli, K. M., Rothbächer, U., Fraser, S. E., & Harland, R. M. (2000) · Nature · doi:10.1038/35011077 · Cited by 751 · see citing works
Shaping the Vertebrate Body Plan by Polarized Embryonic Cell Movements
Keller, R. E. (2002) · Science · doi:10.1126/science.1079478 · Cited by 716 · see citing works
Prespecification and plasticity: shifting mechanisms of cell migration
Friedl, P. (2004) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2003.11.001 · Cited by 670 · see citing works
Non-equilibration of hydrostatic pressure in blebbing cells
Charras, G., Yarrow, J. C., Horton, M. A., Mahadevan, L., & Mitchison, T. J. (2005) · Nature · doi:10.1038/nature03550 · Cited by 657 · see citing works
Reassembly of contractile actin cortex in cell blebs
Charras, G., Hu, C., Coughlin, M. L., & Mitchison, T. J. (2006) · The Journal of Cell Biology · doi:10.1083/jcb.200602085 · Cited by 620 · see citing works
Coactivation of Rac and Rho by Wnt/Frizzled signaling is required for vertebrate gastrulation
Habas, R., Dawid, I. B., & He, X. (2003) · Genes & Development · doi:10.1101/gad.1022203 · Cited by 603 · see citing works
Zebrafish trilobite identifies new roles for Strabismus in gastrulation and neuronal movements
Jessen, J. R., Topczewski, J., Bingham, S., Sepich, D. S., Marlow, F., Chandrasekhar, A., & Solnica-Krezel, L. (2002) · Nature Cell Biology · doi:10.1038/ncb828 · Cited by 504 · see citing works
The Zebrafish Glypican Knypek Controls Cell Polarity during Gastrulation Movements of Convergent Extension
Topczewski, J., Sepich, D. S., Myers, D. C., Walker, C., Amores, A., Lele, Z., Hammerschmidt, M., Postlethwait, J. H., & Solnica‐Krezel, L. (2001) · Developmental Cell · doi:10.1016/s1534-5807(01)00005-3 · Cited by 454 · see citing works
Migration of Zebrafish Primordial Germ Cells: A Role for Myosin Contraction and Cytoplasmic Flow
Blaser, H., Reichman‐Fried, M., Castanon, I., Dumstrei, K., Marlow, F. L., Kawakami, K., Solnica‐Krezel, L., Heisenberg, C., & Raz, E. (2006) · Developmental Cell · doi:10.1016/j.devcel.2006.09.023 · Cited by 406 · see citing works
Dishevelled activates Ca2+ flux, PKC, and CamKII in vertebrate embryos
Sheldahl, L. C., Slusarski, D. C., Pandur, P., Miller, J. R., Kühl, M., & Moon, R. T. (2003) · The Journal of Cell Biology · doi:10.1083/jcb.200211094 · Cited by 350 · see citing works
Planar cell polarization: do the same mechanisms regulate Drosophila tissue polarity and vertebrate gastrulation?
Mlodzik, M. (2002) · Trends in Genetics · doi:10.1016/s0168-9525(02)02770-1 · Cited by 341 · see citing works
Zebrafish Rho Kinase 2 Acts Downstream of Wnt11 to Mediate Cell Polarity and Effective Convergence and Extension Movements
Marlow, F., Topczewski, J., Sepich, D., & Solnica-Krezel, L. (2002) · Current Biology · doi:10.1016/s0960-9822(02)00864-3 · Cited by 337 · see citing works
The role of Ppt/Wnt5 in regulating cell shape and movement during zebrafish gastrulation
Kilian, B., Mansukoski, H., Carreira-Barbosa, F., Ulrich, F., Tada, M., & Heisenberg, C. (2003) · Mechanisms of Development · doi:10.1016/s0925-4773(03)00004-2 · Cited by 327 · see citing works
The Wnt/calcium pathway activates NF-AT and promotes ventral cell fate in Xenopus embryos
Saneyoshi, T., Kume, S., Amasaki, Y., & Mikoshiba, K. (2002) · Nature · doi:10.1038/417295a · Cited by 322 · see citing works
Prickle 1 regulates cell movements during gastrulation and neuronal migration in zebrafish
Carreira-Barbosa, F., Concha, M. L., Takeuchi, M., Ueno, N., Wilson, S. W., & Tada, M. (2003) · Development · doi:10.1242/dev.00567 · Cited by 269 · see citing works
Gravin, an autoantigen recognized by serum from myasthenia gravis patients, is a kinase scaffold protein
Nauert, J. B., Klauck, T. M., Langeberg, L. K., & Scott, J. D. (1997) · Current Biology · doi:10.1016/s0960-9822(06)00027-3 · Cited by 256 · see citing works
Oriented cell divisions and cellular morphogenesis in the zebrafish gastrula and neurula: a time-lapse analysis
Concha, M. L., & Adams, R. J. (1998) · Development · doi:10.1242/dev.125.6.983 · Cited by 237 · see citing works
AKAP signaling complexes at the cytoskeleton
Diviani, D., & Scott, J. D. (2001) · Journal of Cell Science · doi:10.1242/jcs.114.8.1431 · Cited by 227 · see citing works
Shaping the zebrafish notochord
Glickman, N. S., Kimmel, C. B., Jones, M. A., & Adams, R. J. (2003) · Development · doi:10.1242/dev.00314 · Cited by 216 · see citing works
Stat3 Controls Cell Movements during Zebrafish Gastrulation
Yamashita, S., Miyagi, C., Carmany-Rampey, A., Shimizu, T., Fujii, R., Schier, A. F., & Hirano, T. (2002) · Developmental Cell · doi:10.1016/s1534-5807(02)00126-0 · Cited by 178 · see citing works
PKCδ is essential for Dishevelled function in a noncanonical Wnt pathway that regulates Xenopus convergent extension movementsPreprint
Kinoshita, N., Iioka, H., Miyakoshi, A., & Ueno, N. (2003) · Genes & Development · doi:10.1101/gad.1101303 · Cited by 173 · see citing works
Molecular mechanisms of cancer cell invasion and plasticity
Wolf, K., & Friedl, P. (2006) · British Journal of Dermatology · doi:10.1111/j.1365-2133.2006.07231.x · Cited by 163 · see citing works
Noncanonical Wnt signaling regulates midline convergence of organ primordia during zebrafish developmentPreprint
Matsui, T., Raya, Á., Kawakami, Y., Callol-Massot, C., Capdevila, J., Rodrı́guez-Esteban, C., & Belmonte, J. C. I. (2005) · Genes & Development · doi:10.1101/gad.1253605 · Cited by 163 · see citing works
Xenopus Cdc42 Regulates Convergent Extension Movements during Gastrulation through Wnt/Ca2+ Signaling Pathway
Choi, S., & Han, J. (2002) · Developmental Biology · doi:10.1006/dbio.2002.0602 · Cited by 160 · see citing works
A Novel src- and ras-suppressed Protein Kinase C Substrate Associated with Cytoskeletal Architecture
Lin, X., Tombler, E., Nelson, P. J., Ross, M. J., & Gelman, I. H. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.45.28430 · Cited by 155 · see citing works
Dynamic modes of the cortical actomyosin gel during cell locomotion and division
Paluch, E. K., Sykes, C., Prost, J., & Bornens, M. (2005) · Trends in Cell Biology · doi:10.1016/j.tcb.2005.11.003 · Cited by 142 · see citing works
Has2 is required upstream of Rac1 to govern dorsal migration of lateral cells during zebrafish gastrulation
Bakkers, J., Kramer, C., Pothof, J., Quaedvlieg, N. E. M., Spaink, H. P., & Hammerschmidt, M. (2004) · Development · doi:10.1242/dev.00954 · Cited by 137 · see citing works
The Golgi apparatus and the centrosome are localized to the sites of newly emerging axons in cerebellar granule neurons in vitro
Zmuda, J. F., & Rivas, R. J. (1998) · Cell Motility and the Cytoskeleton · doi:10.1002/(sici)1097-0169(1998)41:1 · Cited by 125 · see citing works
The Src-suppressed C kinase substrate, SSeCKS, is a potential metastasis inhibitor in prostate cancer.
Xia, W., Unger, P., Miller, L., Nelson, J. B., & Gelman, I. H. (2001) · PubMed · Cited by 125 · see citing works
Gastrulation in zebrafish — all just about adhesion?
Solnica‐Krezel, L. (2006) · Current Opinion in Genetics & Development · doi:10.1016/j.gde.2006.06.009 · Cited by 124 · see citing works
Essential roles of Gα12/13 signaling in distinct cell behaviors driving zebrafish convergence and extension gastrulation movements
Lin, F. H., Sepich, D. S., Chen, S., Topczewski, J., Yin, C., Solnica‐Krezel, L., & Hamm, H. E. (2005) · The Journal of Cell Biology · doi:10.1083/jcb.200501104 · Cited by 107 · see citing works
Initiation of convergence and extension movements of lateral mesoderm during zebrafish gastrulation
Sepich, D. S., Calmelet, C., Kiskowski, M. A., & Solnica‐Krezel, L. (2005) · Developmental Dynamics · doi:10.1002/dvdy.20507 · Cited by 98 · see citing works
Blebbing of Dictyostelium cells in response to chemoattractant
Langridge, P. D., & Kay, R. R. (2006) · Experimental Cell Research · doi:10.1016/j.yexcr.2006.03.007 · Cited by 90 · see citing works
Fyn/Yes and non‐canonical Wnt signalling converge on RhoA in vertebrate gastrulation cell movements
Jopling, C., & den Hertog, J. (2005) · EMBO Reports · doi:10.1038/sj.embor.7400386 · Cited by 79 · see citing works
On the convergent cell movements of gastrulation in Fundulus
Trinkaus, J. P., Trinkaus, M., & Fink, R. D. (1992) · Journal of Experimental Zoology · doi:10.1002/jez.1402610107 · Cited by 79 · see citing works
Combinatorial activity of Flamingo proteins directs convergence and extension within the early zebrafish embryo via the planar cell polarity pathway
Formstone, C. J., & Mason, I. J. (2005) · Developmental Biology · doi:10.1016/j.ydbio.2005.03.026 · Cited by 74 · see citing works
Reexpression of the major protein kinase C substrate, SSeCKS, suppresses v-src-induced morphological transformation and tumorigenesis.
Lin, X. L., & Gelman, I. H. (1997) · PubMed · Cited by 72 · see citing works
A Role for SSeCKS, a Major Protein Kinase C Substrate with Tumour Suppressor Activity, in Cytoskeletal Architecture, Formation of Migratory Processes, and Cell Migration During Embryogenesis
Gelman, I. H., Tombler, E., & Vargas, J. (2000) · The Histochemical Journal · doi:10.1023/a:1003950027529 · Cited by 57 · see citing works
Rho mediates cytokinesis and epiboly via ROCK in zebrafish
Lai, S.-L., Chang, C.-N., Wang, P.-J., & Lee, S. (2005) · Molecular Reproduction and Development · doi:10.1002/mrd.20290 · Cited by 49 · see citing works
Functional interaction between the SSeCKS scaffolding protein and the cytoplasmic domain of β1,4-galactosyltransferase
Wassler, M. J., Foote, C. I., Gelman, I. H., & Shur, B. D. (2001) · Journal of Cell Science · doi:10.1242/jcs.114.12.2291 · Cited by 46 · see citing works
Developmentally Restricted Actin-Regulatory Molecules Control Morphogenetic Cell Movements in the Zebrafish Gastrula
Daggett, D. F., Boyd, C. A., Gautier, P. E., Bryson‐Richardson, R. J., Thisse, C., Thisse, B., Amacher, S. L., & Currie, P. D. (2004) · Current Biology · doi:10.1016/j.cub.2004.08.024 · Cited by 43 · see citing works
Role of PKA as a negative regulator of PCP signaling pathway during Xenopus gastrulation movements
Park, E., Kim, G.-H., Choi, S.-C., & Han, J.-K. (2006) · Developmental Biology · doi:10.1016/j.ydbio.2006.01.011 · Cited by 41 · see citing works
SSeCKS/Gravin/AKAP12 Metastasis Suppressor Inhibits Podosome Formation via RhoA- and Cdc42-Dependent Pathways
Gelman, I. H., & Gao, L. (2006) · Molecular Cancer Research · doi:10.1158/1541-7786.mcr-05-0252 · Cited by 41 · see citing works
Zebrafish gastrulation movements: bridging cell and developmental biology
Heisenberg, C., & Tada, M. (2002) · Seminars in Cell and Developmental Biology · doi:10.1016/s1084952102001003 · Cited by 41 · see citing works
Rho guanine nucleotide exchange factor xNET1 implicated in gastrulation movements during Xenopus development
Miyakoshi, A., Ueno, N., & Kinoshita, N. (2004) · Differentiation · doi:10.1111/j.1432-0436.2004.07201004.x · Cited by 38 · see citing works
Analysis of Cell Movements in Zebrafish Embryos: Morphometrics and Measuring Movement of Labeled Cell Populations In Vivo
Sepich, D. S., & Solnica‐Krezel, L. (2004) · Humana Press eBooks · doi:10.1385/1-59259-860-9:211 · Cited by 26 · see citing works
Cross-species Sequence Analysis Reveals Multiple Charged Residue-rich Domains That Regulate Nuclear/Cytoplasmic Partitioning and Membrane Localization of A Kinase Anchoring Protein 12 (SSeCKS/Gravin)
Streb, J. W., & Miano, J. M. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m414017200 · Cited by 23 · see citing works
A β1,4-galactosyltransferase is required for convergent extension movements in zebrafish
Machingo, Q. J., Fritz, A., & Shur, B. D. (2006) · Developmental Biology · doi:10.1016/j.ydbio.2006.05.024 · Cited by 21 · see citing works
Xgravin-like (Xgl), a novel putative a-kinase anchoring protein (AKAP) expressed during embryonic development in Xenopus
Klingbeil, P., Frazzetto, G., & Bouwmeester, T. (2001) · Mechanisms of Development · doi:10.1016/s0925-4773(00)00527-x · Cited by 17 · see citing works
Genetic analysis of the exed region in mouse
Kendall, S. K., Strong, S. J., Litman, R. T., Litman, G. W., & Magnuson, T. (2000) · genesis · doi:10.1002/1526-968x(200008)27:4

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Retracted
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Réactivation des gènes embryonnaires twist et snai3 et dissémination précoce des cellules cancéreuses
Bastid, J. (2009) · doi:10.70675/1f08a215z4b9cz4886z8897z7926d12b1bff

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Chapter 7 Convergence and Extension Movements During Vertebrate Gastrulation
Yin, C., Ciruna, B. G., & Solnica‐Krezel, L. (2009) · Current topics in developmental biology/Current Topics in Developmental Biology · doi:10.1016/s0070-2153(09)89007-8 · Cited by 67 · see citing works
Wnt Signalling and Regulation of Gastrulation Movements
Shi, D. (2004) · Springer eBooks · doi:10.1007/978-3-662-10416-3_12
Cellular and molecular mechanisms of convergence and extension in zebrafish
Williams, M. K., & Solnica‐Krezel, L. (2020) · Current topics in developmental biology/Current Topics in Developmental Biology · doi:10.1016/bs.ctdb.2019.08.001 · Cited by 47 · see citing works

Authors: Weiser, D. C., Pyati, U. J., & Kimelman, D.
Affiliations: University of Washington (US)
ORCID: Weiser, Douglas C.
Kimelman, David
Venue: Genes & Development
Publisher: Cold Spring Harbor Laboratory
Published: 2007-06-15
Volume: 21
Issue: 12
Pages: 1559-1571
ISSN: 0890-9369 (ISSN-L)
1549-5477
DOI: 10.1101/gad.1535007
PMC: PMC1891432
OpenAlex: W2051173163
Cited by: 62
References: 59
Citations by year: 2025: 3 · 2023: 3 · 2022: 3 · 2021: 2 · 2020: 1 · 2019: 1 · 2017: 2 · 2016: 4 · 2015: 8 · 2014: 3 · 2013: 3 · 2012: 5
Type: posted content
Language: en
Open access: bronze
doi.org
Locations: Genes & Development (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: National Institutes of Health
Eunice Kennedy Shriver National Institute of Child Health and Human Development (R01 HD27262, R01HD027262, F32HD053189)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
Keywords: gravin, gastrulation, convergent extension, mesoderm, body axis elongation, cell migration, cell intercalation, paraxial mesoderm, scaffold proteins, tumor suppressor, zebrafish embryos, metastasis
MeSH terms: Amino Acid Sequence, Animals, Base Sequence, Cell Movement, Gastrula, Mesoderm, Models, Biological, Molecular Sequence Data, Proteins, RNA, Messenger, Zebrafish, Sequence Homology, Amino Acid, Gene Expression Regulation, Developmental, Cell Cycle Proteins, Body Patterning, rhoA GTP-Binding Protein, Myosin Type II, Zebrafish Proteins, Cell Shape, A Kinase Anchor Proteins

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Gravin regulates mesodermal cell behavior changes required for axis elongation during zebrafish gastrulation

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