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Cited by 1,013Developmental Biology and Gene RegulationWnt/β-catenin signaling in development and cancerRenal and related cancers

Silberblick/Wnt11 mediates convergent extension movements during zebrafish gastrulation

Heisenberg, Carl-Philipp, Tada, Masazumi, Rauch, Gerd-Jörg, Saúde, Leonor, Concha, Miguel L., Geisler, Robert, Stemple, Derek L., Smith, James C. +1 more·2000·Nature
DOI 10.1038/35011068
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Abstract

Vertebrate gastrulation involves the specification and coordinated movement of large populations of cells that give rise to the ectodermal, mesodermal and endodermal germ layers. Although many of the genes involved in the specification of cell identity during this process have been identified, little is known of the genes that coordinate cell movement. Here we show that the zebrafish silberblick (slb) locus encodes Wnt11 and that Slb/Wnt11 activity is required for cells to undergo correct convergent extension movements during gastrulation. In the absence of Slb/Wnt11 function, abnormal extension of axial tissue results in cyclopia and other midline defects in the head. The requirement for Slb/Wnt11 is cell non-autonomous, and our results indicate that the correct extension of axial tissue is at least partly dependent on medio-lateral cell intercalation in paraxial tissue. We also show that the slb phenotype is rescued by a truncated form of Dishevelled that does not signal through the canonical Wnt pathway, suggesting that, as in flies, Wnt signalling might mediate morphogenetic events through a divergent signal transduction cascade. Our results provide genetic and experimental evidence that Wnt activity in lateral tissues has a crucial role in driving the convergent extension movements underlying vertebrate gastrulation.

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Crossref · 10.1038/35011068

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  • The evidence in zebrafish demonstrated that injection of Wnt11 RNA into the two-cell stage embryo was sufficient to rescue the gastrulation defects observed in the silberblick mutant, indicating that localized expression of Wnt11 was not required and thus its role was permissive.

    “The evidence in zebrafish demonstrated that injection of Wnt11 RNA into the two-cell stage embryo was sufficient to rescue the gastrulation defects observed in the silberblick mutant, indicating that localized expression of Wnt11 was not required and thus its role was permissive.89”

    From Wnt signal transduction pathways (2008) · cites this paper as [89]

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Authors
Heisenberg, C.-P., Tada, M., Rauch, G.-J., Saúde, L., Concha, M. L., Geisler, R., Stemple, D. L., Smith, J. C., & Wilson, S. W.
Affiliations
  • University College London (GB)
  • National Institute for Medical Research (GB)
  • Max Planck Institute for Developmental Biology (DE)
Corresponding author
Heisenberg, Carl-Philipp
ORCID
Venue
Nature
Publisher
Springer Science and Business Media LLC
Published
2000-05
Volume
405
Issue
6782
Pages
76-81
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
DOI
10.1038/35011068
OpenAlex
W1974712344
Cited by
1,013
References
31
Citations by year
2026: 17 · 2025: 23 · 2024: 19 · 2023: 19 · 2022: 25 · 2021: 29 · 2020: 23 · 2019: 23 · 2018: 25 · 2017: 29 · 2016: 29 · 2015: 36 · 2014: 34 · 2013: 34 · 2012: 46
FWCI
20.38
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Nature (publishedVersion)
  • PubMed (publishedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
  • UCL Discovery (University College London) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Renal and related cancers
Keywords
Wnt11, convergent extension, gastrulation, zebrafish, cyclopia, midline defects, cell intercalation, Dishevelled, non-canonical Wnt signaling, cell motility, germ layer formation
MeSH terms
Animals, Cell Movement, Embryo, Nonmammalian, Gastrula, Glycoproteins, Mutation, Zebrafish, Signal Transduction, Zebrafish Proteins, Wnt Proteins

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