The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
Pimet, the Drosophila homolog of HEN1, mediates 2′-O-methylation of Piwi- interacting RNAs at their 3′ ends
Open accessAbstract
Piwi-interacting RNAs (piRNAs) consist of a germline-specific group of small RNAs derived from distinct intergenic loci in the genome. piRNAs function in silencing selfish transposable elements through binding with the PIWI subfamily proteins of Argonautes. Here we show that piRNAs in Drosophila are 2′-O-methylated at their 3′ ends. Loss of Pimet (piRNA methyltransferase), the Drosophila homolog of Arabidopsis HEN1 methyltransferase for microRNAs (miRNAs), results in loss of 2′-O-methylation of fly piRNAs. Recombinant Pimet shows single-stranded small RNA methylation activity in vitro and interacts with the PIWI proteins within Pimet mutant ovary. These results show that Pimet mediates piRNA 2′-O-methylation in Drosophila.
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A Distinct Small RNA Pathway Silences Selfish Genetic Elements in the Germline
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A Slicer-Mediated Mechanism for Repeat-Associated siRNA 5' End Formation in Drosophila
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Self-citationMethylation as a Crucial Step in Plant microRNA Biogenesis
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A Role for Piwi and piRNAs in Germ Cell Maintenance and Transposon Silencing in Zebrafish
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Metadata
- Authors
- Saito, K., Sakaguchi, Y., Suzuki, T., Suzuki, T., Siomi, H., & Siomi, M. C.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2007-07-01
- Volume
- 21
- Issue
- 13
- Pages
- 1603-1608
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2089053855
- Cited by
- 462
- References
- 33
- Type
- posted content
- Language
- en
- Open access
- diamond
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- CoChat. (2026). Citation check: Pimet, the Drosophila homolog of HEN1, mediates 2′-O-methylation of Piwi- interacting RNAs at their 3′ ends. https://app.cochat.ai/papers/10.1101/gad.1563607
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- Saito, K., Sakaguchi, Y., Suzuki, T., Suzuki, T., Siomi, H., & Siomi, M. C. (2007). Pimet, the Drosophila homolog of HEN1, mediates 2′-O-methylation of Piwi- interacting RNAs at their 3′ ends. Genes & Development. https://doi.org/10.1101/gad.1563607
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