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Target RNA–Directed Trimming and Tailing of Small Silencing RNAs

Ameres, Stefan L., Horwich, Michael D., Hung, Jui-Hung, Xu, Jia, Ghildiyal, Megha, Weng, Zhiping, Zamore, Phillip D.·2010·Science
DOI 10.1126/science.1187058PMC PMC2902985
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Abstract

Close, But Not Too Close: MicroRNAs (miRNAs) in plants are generally highly complementary to their target RNAs, yet, in most animal miRNAs, only the ∼8-nucleotide “seeds” sequence bases pair fully with the target, with few base pairs between the remainder of the miRNA and target. Plant miRNAs are methylated at their 3′ ends, whereas animals' miRNAs are not. Ameres et al. (p. 1534 ; see the Perspective by Pasquinelli ) noticed that, in fruit flies, miRNAs engineered to have high complementarity to target RNAs were present at reduced levels. These miRNAs were trimmed and uridylated at their 3′ ends, features involved in RNA degradation. Fly small interfering RNAs, all of which are methylated at their 3′ ends, were unaffected, unless the methylating enzyme, Hen1, was mutated. Thus, 3′-methylation may prevent complementarity-driven remodeling and degradation of small RNAs.

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  • Under TDMD, the presence of a target mRNA promotes cognate miRNA degradation, often with specific target mRNAs responsible for controlling miRNA decay.

    “Under TDMD, the presence of a target mRNA promotes cognate miRNA degradation, often with specific target mRNAs responsible for controlling miRNA decay (Ameres et al., 2010; Cazalla, Yario, Steitz, & Steitz, 2010; de la Mata et al., 2015; Ghini et al., 2018; Kleaveland, Shi, Stefano, & Bartel, 2018).”

    From microRNA strand selection: Unwinding the rules (2021) · cites this paper as Ameres et al., 2010

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Authors
Ameres, S. L., Horwich, M. D., Hung, J.-H., Xu, J., Ghildiyal, M., Weng, Z., & Zamore, P. D.
Affiliations
  • Howard Hughes Medical Institute and Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA.
  • Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA.
  • Program in Bioinformatics and Integrative Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA.
Venue
Science
Publisher
American Association for the Advancement of Science (AAAS)
Published
2010-06-18
Volume
328
Issue
5985
Pages
1534-1539
ISSN
  • 0036-8075
  • 1095-9203
DOI
10.1126/science.1187058
PMC
PMC2902985
Cited by
597
References
1
Language
en

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