The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Mouse ES cells express endogenous shRNAs, siRNAs, and other Microprocessor-independent, Dicer-dependent small RNAs
Open accessAbstract
Canonical microRNAs (miRNAs) require two processing steps: the first by the Microprocessor, a complex of DGCR8 and Drosha, and the second by a complex of TRBP and Dicer. dgcr8 Δ/Δ mouse embryonic stem cells (mESCs) have less severe phenotypes than dicer1 Δ/Δ mESCs, suggesting a physiological role for Microprocessor-independent, Dicer-dependent small RNAs. To identify these small RNAs with unusual biogenesis, we performed high-throughput sequencing from wild-type, dgcr8 Δ/Δ, and dicer1 Δ/Δ mESCs. Several of the resulting DGCR8-independent, Dicer-dependent RNAs were noncanonical miRNAs. These derived from mirtrons and a newly identified subclass of miRNA precursors, which appears to be the endogenous counterpart of shRNAs. Our analyses also revealed endogenous siRNAs resulting from Dicer cleavage of long hairpins, the vast majority of which originated from one genomic locus with tandem, inverted short interspersed nuclear elements (SINEs). Our results extend the known diversity of mammalian small RNA-generating pathways and show that mammalian siRNAs exist in cell types other than oocytes.
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Cites
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Cited by
Regulation of microRNA biogenesis
Ha, M., & Kim, V. N. (2014) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3838 · Cited by 5,462
Overview of MicroRNA Biogenesis, Mechanisms of Actions, and Circulation
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Metadata
- Authors
- Babiarz, J. E., Ruby, J. G., Wang, Y., Bartel, D. P., & Blelloch, R.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2008-10-15
- Volume
- 22
- Issue
- 20
- Pages
- 2773-2785
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2155412493
- Cited by
- 908
- References
- 9
- Type
- posted content
- Language
- en
- Open access
- diamond
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- Babiarz, J. E., Ruby, J. G., Wang, Y., Bartel, D. P., & Blelloch, R. (2008). Mouse ES cells express endogenous shRNAs, siRNAs, and other Microprocessor-independent, Dicer-dependent small RNAs. Genes & Development. https://doi.org/10.1101/gad.1705308
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