The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns.
Open accessAbstract
A protein of molecular size 62,000 daltons (p62) was detected in HeLa cell nuclear extracts by UV cross-linking to mRNA precursors. p62 binds specifically to the polypyrimidine tract of the 3' splice site region of introns. p62 purified to homogeneity binds the polypyrimidine tract of pre-mRNAs. This binding does not require the AG dinucleotide at the 3' splice site. Alterations in the polypyrimidine tract that reduce the binding of p62 yield a corresponding reduction in the efficiency of formation of a U2 snRNP/pre-mRNA complex and splicing. The p62 protein is retained in the spliceosome, where it remains bound to the pre-mRNA. This polypyrimidine tract binding protein (pPTB) is proposed to be a critical component in recognition of the 3' splice site during splicing.
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Cites
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Self-citationSplicing of Messenger RNA Precursors
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Self-citationSplicing of Messenger RNA Precursors
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Cited by
Mechanisms of Alternative Pre-Messenger RNA Splicing
Black, D. L. (2003) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.72.121801.161720 · Cited by 2,704
Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus
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Singh, R., Valcárcel, J., & Green, M. R. (1995) · Science · doi:10.1126/science.7761834 · Cited by 537
Site-Specific Modification of Pre-mRNA: the 2′-Hydroxyl Groups at the Splice Sites
Moore, M. J., & Sharp, P. A. (1992) · Science · doi:10.1126/science.1589782 · Cited by 469
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Metadata
- Authors
- Garcia-Blanco, M. A., Jamison, S. F., & Sharp, P. A.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 1989-12-01
- Volume
- 3
- Issue
- 12a
- Pages
- 1874-1886
- ISSN
- 0890-9369 (ISSN-L)
- OpenAlex
- W2125304732
- Cited by
- 332
- References
- 39
- Type
- posted content
- Language
- en
- Open access
- diamond
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- CoChat. (2026). Citation check: Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns. https://app.cochat.ai/papers/10.1101/gad.3.12a.1874
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- Garcia-Blanco, M. A., Jamison, S. F., & Sharp, P. A. (1989). Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns. Genes & Development. https://doi.org/10.1101/gad.3.12a.1874
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
