The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA
Open accessAbstract
RNA-guided endonucleases (RGENs) derived from the CRISPR/Cas system represent an efficient tool for genome editing. RGENs consist of two components: Cas9 protein and guide RNA. Plasmid-mediated delivery of these components into cells can result in uncontrolled integration of the plasmid sequence into the host genome, and unwanted immune responses and potential safety problems that can be caused by the bacterial sequences. Furthermore, this delivery method requires transfection tools. Here we show that simple treatment with cell-penetrating peptide (CPP)-conjugated recombinant Cas9 protein and CPP-complexed guide RNAs leads to endogenous gene disruptions in human cell lines. The Cas9 protein was conjugated to CPP via a thioether bond, whereas the guide RNA was complexed with CPP, forming condensed, positively charged nanoparticles. Simultaneous and sequential treatment of human cells, including embryonic stem cells, dermal fibroblasts, HEK293T cells, HeLa cells, and embryonic carcinoma cells, with the modified Cas9 and guide RNA, leads to efficient gene disruptions with reduced off-target mutations relative to plasmid transfections, resulting in the generation of clones containing RGEN-induced mutations. Our CPP-mediated RGEN delivery process provides a plasmid-free and additional transfection reagent-free method to use this tool with reduced off-target effects. We envision that our method will facilitate RGEN-directed genome editing.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The cited source doesn’t support the claim as written.
In the citing paper
“RNP electroporation exhibits higher on-target editing efficiency and lower off-target mutations than the other delivery methods”
From Off-target effects in CRISPR/Cas9 gene editing (2023) · cites this paper as Ramakrishna et al., 2014
Checked by a reasoning model — may be wrong.
Experimental — accuracy not yet measuredThe source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“A powerful advantage of the CRISPR/Cas9 system is the ability to target any ∼23-bp sequence that contains a PAM motif on either strand of DNA.”
From Delivering CRISPR: a review of the challenges and approaches (2018) · cites this paper as Ramakrishna et al., 2014
Experimental — accuracy not yet measuredThe source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“cell-penetrating peptides (CPP), conjugated with Cas9 and complexed with gRNA enabled efficient gene silencing in human cell lines and disease models featuring fewer off-target effects when compared to plasmid transfection [153,154].”
From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [153]
Experimental — accuracy not yet measuredIn the citing paper
“Ramakrishna et al. demonstrated that conjugating Cas9 and sgRNA with cell-penetrating peptides (CPPs) enhanced intracellular delivery efficiency because of the membrane-disturbed properties of CPPs.[227]”
From RNA delivery biomaterials for the treatment of genetic and rare diseases (2019) · cites this paper as [227]
In the citing paper
“Some work with CPPs and CRISPR/Cas9 was accomplished as early as 2014 by Ramakrishna et al. (2014), who showed separate delivery of CPP–Cas9 and CPP–sgRNA to multiple human cell lines.”
From Delivering CRISPR: a review of the challenges and approaches (2018) · cites this paper as Ramakrishna et al., 2014
In the citing paper
“Ramakrishna et al. developed cell-penetrating peptide (CPP)-conjugated recombinant Cas9 protein and CPP-complexed guide RNAs for gene disruptions of CCR5 locus in human cell lines, including HEK293T, HeLa, NCCIT (a human pluripotent embryonal carcinoma cell line), human dermal fibroblasts and human embryonic stem cells.”
From Non-viral delivery systems for CRISPR/Cas9-based genome editing: challenges and opportunities (2018) · cites this paper as [96]
In the citing paper
“Cell-penetrate-peptide-mediated delivery was reported to achieve higher on-to off-target activity ratio compared with plasmid-mediated delivery.53”
From CRISPR-Cas9: a new and promising player in gene therapy (2015) · cites this paper as [53]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116
Multiplex Genome Engineering Using CRISPR/Cas Systems
Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339
RNA-Guided Human Genome Engineering via Cas9
Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210
A Toll-like receptor recognizes bacterial DNA
Hemmi, H., Takeuchi, O., Kawai, T., Kaisho, T., Sato, S., Sanjo, H., Matsumoto, M., Hoshino, K., Wagner, H., Takeda, K., et al. (2000) · Nature · doi:10.1038/35047123 · Cited by 6,491 · cited 3×
“responses (Hemmi et al. 2000; Wagner 2001) caused by bacterial sequences, and safety problems if the antibiotic-resistance gene is taken up by pathogenic bacteria.”
BackgroundInfluentialDNA targeting specificity of RNA-guided Cas9 nucleases
Hsu, P. D., Scott, D. A., Weinstein, J. A., Ran, F. A., Konermann, S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2647 · Cited by 4,741
Wang, H., Yang, H., Shivalila, C. S., Dawlaty, M. M., Cheng, A. W., Zhang, F., & Jaenisch, R. (2013) · Cell · doi:10.1016/j.cell.2013.04.025 · Cited by 3,495
Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity
Ran, F. A., Hsu, P. D., Lin, C.-Y., Gootenberg, J. S., Konermann, S., Trevino, A. E., Scott, D. A., Inoue, A., Matoba, S., Zhang, Y., et al. (2013) · Cell · doi:10.1016/j.cell.2013.08.021 · Cited by 3,299
High-Frequency off-Target Mutagenesis Induced by CRISPR-Cas Nucleases in Human Cells
Fu, Y., Foden, J. A., Khayter, C., Maeder, M. L., Reyon, D., Joung, J. K., & Sander, J. D. (2013) · Nature Biotechnology · doi:10.1038/nbt.2623 · Cited by 3,292
Efficient genome editing in zebrafish using a CRISPR-Cas system
Hwang, W. Y., Fu, Y., Reyon, D., Maeder, M. L., Tsai, S. Q., Sander, J. D., Peterson, R. T., Yeh, J.-R. J., & Joung, J. K. (2013) · Nature Biotechnology · doi:10.1038/nbt.2501 · Cited by 2,937
CRISPR/Cas, the Immune System of Bacteria and Archaea
Horvath, P., & Barrangou, R. (2010) · Science · doi:10.1126/science.1179555 · Cited by 2,471
Flagged first, then by citations
Cited by
Sorted by citations
Items 501–600 of 653
Ghosh, A., Myacheva, K., Riester, M., Schmidt, C., & Diederichs, S. (2022) · RNA Biology · doi:10.1080/15476286.2022.2067713 · Cited by 6
Nanoparticle-based CRISPR/Cas Delivery: An Emerging Tactic for Cancer Therapy
Dilnawaz, F., & Acharya, S. (2022) · Current Medicinal Chemistry · doi:10.2174/0929867329666221006112615 · Cited by 7
Production of Duchenne muscular dystrophy cellular model using CRISPR-Cas9 exon deletion strategy
Alizadeh, F. L., Abraghan, Y. J., Farrokhi, S., Yousefi, Y., Mirahmadi, Y., Eslahi, A., & Mojarrad, M. (2023) · Molecular and Cellular Biochemistry · doi:10.1007/s11010-023-04759-3 · Cited by 7
Lentiviral Transduction-based CRISPR/Cas9 Editing of Schistosoma mansoni Acetylcholinesterase
Du, X., McManus, D. P., French, J. D., Sivakumaran, H., Johnston, R. L., Kondrashova, O., Fogarty, C. E., Jones, M. K., & You, H. (2023) · Current Genomics · doi:10.2174/1389202924666230823094608 · Cited by 6
Huang, Y., Zhu, W., Zhou, J., Huang, Q., & Zeng, G. (2024) · Biomolecules · doi:10.3390/biom14050511 · Cited by 7
The CRISPR/Cas System: Emerging Technology and Application
(2016) · Caister Academic Press eBooks · doi:10.21775/9781910190630 · Cited by 6
Delivery of Superoxide Dismutase Using Cys2-His2 Zinc-Finger Proteins
Liu, J., Li, J., Li, J., Zhu, L., Wang, S., Xuan, W., & Ma, P. (2018) · Methods in molecular biology · doi:10.1007/978-1-4939-8799-3_9 · Cited by 6
Nonviral Gene Therapy: Peptiplexes
Tomich, J. M., Wessel, E., Choi, J., & Avila, L. A. (2019) · Elsevier eBooks · doi:10.1016/b978-0-12-814470-1.00008-3 · Cited by 6
Gene Therapy in Pediatric Liver Disease
Muro, A. F., D’Antiga, L., & Mingozzi, F. (2019) · Springer eBooks · doi:10.1007/978-3-319-96400-3_44 · Cited by 6
Get ready for the CRISPR/Cas system: A beginner's guide to the engineering and design of guide RNAs
Ma, S., Lv, J., Feng, Z., Rong, Z., & Lin, Y. (2021) · The Journal of Gene Medicine · doi:10.1002/jgm.3377 · Cited by 8
Gene delivery into cells and tissues
Nelson, C. E., Duvall, C. L., Prokop, A., Gersbach, C. A., & Davidson, J. M. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-818422-6.00030-7 · Cited by 6
Single-cell synaptome mapping of endogenous protein subpopulations in mammalian brain
Uchigashima, M., Iguchi, R., Fujii, K., Shiku, K., Kumar, P., Liu, X.-Y., Isogai, M., Hoshino, C., Abe, M., Nozumi, M., et al. (2025) · Nature Communications · doi:10.1038/s41467-025-65813-w · Cited by 6
Efficient prime editing in vivo and in vitro using lipid nanoparticles
Jiang, A. Y., Cristian, A., Brooks, D. L., Feierman, E. R., Chen, P., Whittaker, M. N., Pierce, S. E., Sakai, H. A., Chen, H., Liu, D., et al. (2026) · Nature Nanotechnology · doi:10.1038/s41565-026-02200-6 · Cited by 5
Saito, A. C., Higashi, T., & Chiba, H. (2024) · STAR Protocols · doi:10.1016/j.xpro.2024.103179 · Cited by 5
CRISPR/Cas systems for genomic Editing, biochemical Sensing, Bioanalysis, and diagnostics
Baig, M. M. F. A., Chair, S. Y., & Chien, W. T. (2025) · Microchemical Journal · doi:10.1016/j.microc.2024.112638 · Cited by 5
Non-viral systems for intracellular delivery of genome editing tools
Shaikhutdinov, I. K., Ilyasov, P. V., Витальевна, Г. О., & Limareva, L. V. (2024) · Vavilov Journal of Genetics and Breeding · doi:10.18699/vjgb-24-28 · Cited by 5
The Synergy between CRISPR and Chemical Engineering
Lau, C., & Tin, C. (2019) · Current Gene Therapy · doi:10.2174/1566523219666190701100556 · Cited by 5
CRISPR/Cas9 mediated genome engineering in microbes and its application in plant beneficial effects
Alok, A., Tiwari, S., & Kaur, J. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-818469-1.00028-6 · Cited by 5
Gopalappa, R., Song, M., Chandrasekaran, A. P., Das, S., Haq, S., Koh, H. C., & Ramakrishna, S. (2018) · Archives of Pharmacal Research · doi:10.1007/s12272-018-1042-2 · Cited by 5
Self-citationClasses and Applications of Cell-Penetrating Peptides
Langel, Ü. (2019) · Springer eBooks · doi:10.1007/978-981-13-8747-0_2 · Cited by 6
Chen, S., Nguyen, A., Müller, J. T., Molbay, M., Mehta, A., Sheshachala, S., Baskaya, K., Adams, N. B. P., Carneiro, S. P., & Merkel, O. M. (2025) · European Journal of Pharmaceutical Sciences · doi:10.1016/j.ejps.2025.107090 · Cited by 4
Almasoudi, H. H. (2025) · Current Research in Translational Medicine · doi:10.1016/j.retram.2025.103513 · Cited by 5
CRISPR libraries: A powerful screening tool for function and mechanism study of cancer
Jia, C., Liang, Z., Yao, X., Lin, Q., & Zhang⋆, Y. (2025) · Cell investigation. · doi:10.1016/j.clnves.2025.100044 · Cited by 6
Cell‐Permeable Ubiquitin and Histone Tools for Studying Post‐translational Modifications
Tang, J., Shu, Q., Guo, Y., Zhu, H., & Li, Y. (2023) · ChemBioChem · doi:10.1002/cbic.202300169 · Cited by 4
Rapid Control of Genome Editing in Human Cells by Chemical-Inducible CRISPR-Cas Systems
Liu, K. I., Ramli, M. N., Sutrisnoh, N.-A. B., & Tan, M. H. (2018) · Methods in molecular biology · doi:10.1007/978-1-4939-7795-6_15 · Cited by 4
Notes on Functional Modules in the Assembly of CRISPR/Cas9-Mediated Epigenetic Modifiers
Kondrashov, A. V., & Karpova, E. (2020) · Methods in molecular biology · doi:10.1007/978-1-0716-0876-0_30 · Cited by 4
Tumor-on-a-chip devices for cancer immunotherapy
Mu, X., & Zhang, Y. S. (2021) · Elsevier eBooks · doi:10.1016/b978-0-323-90949-5.00006-1 · Cited by 4
Shalaby, K. E., Aouida, M., Gupta, V. R., Ghanem, S. S., & El‐Agnaf, O. M. A. (2022) · Frontiers in Genome Editing · doi:10.3389/fgeed.2022.854866 · Cited by 6
Kim, Y. J., Lee, H., Cha, H., & Park, J. H. (2020) · Biotechnology and Bioprocess Engineering · doi:10.1007/s12257-020-0068-8 · Cited by 4
Wu, H., Chaudhary, A., & Mahato, R. I. (2019) · Springer eBooks · doi:10.1007/978-3-030-00710-2_16 · Cited by 4
Genome Editing with Targetable Nucleases
Pelletier, S. W. (2016) · Springer eBooks · doi:10.1007/978-3-319-34148-4_1 · Cited by 4
Tan, Z. Y., Huang, T., & Ngeow, J. Y. Y. (2018) · Endocrine Related Cancer · doi:10.1530/erc-18-0039 · Cited by 5
Kaushal, K., Woo, S. H., Tyagi, A., Kim, D. H., Suresh, B., Kim, K.-S., & Ramakrishna, S. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21228719 · Cited by 4
Self-citationUbiquitin specific protease 7 deubiquitinates and regulates Aurora B-mediated cytokinesis
Kaushal, K., Antao, A. M., Das, S., Kim, S. L., Birappa, G., Rajkumar, S., Gowda, D. A. A., Ajaykumar, C., Singh, V., Kim, K., et al. (2025) · BMB Reports · doi:10.5483/bmbrep.2024-0154 · Cited by 3
Self-citationCRISPR-based antimicrobials and nanomotor technologies for drug-resistant biofilms
Sun, Q., Kawuribi, V., Xie, Y., Xu, H., & Zheng, S. (2026) · Journal of Drug Delivery Science and Technology · doi:10.1016/j.jddst.2026.108002 · Cited by 3
Tassaneetrithep, B., Phuphuakrat, A., Pasomsub, E., Bhukhai, K., Wongkummool, W., Priengprom, T., Khamaikawin, W., Chaisavaneeyakorn, S., Anurathapan, U., Apiwattanakul, N., et al. (2024) · Heliyon · doi:10.1016/j.heliyon.2024.e26613 · Cited by 3
Therapeutic Gene Editing in Muscles and Muscle Stem Cells
Tabebordbar, M., Cheng, J. K. W., & Wagers, A. J. (2017) · Research and perspectives in neurosciences · doi:10.1007/978-3-319-60192-2_10 · Cited by 3
Techniques and strategies employing engineered transcription factors
Lawson, S., & Bleris, L. G. (2017) · Current Opinion in Biomedical Engineering · doi:10.1016/j.cobme.2017.10.009 · Cited by 3
RNA-based CRISPR-Mediated Loss-of-Function Mutagenesis in Human Pluripotent Stem Cells
Leung, A. W., Broton, C., Bogacheva, M. S., Xiao, A., García‐Castro, M. I., & Lou, Y. (2020) · Journal of Molecular Biology · doi:10.1016/j.jmb.2020.04.017 · Cited by 3
Recent Advancement and Innovations in CRISPR/Cas and CRISPR Related Technologies: A review
Khan, S. H. (2021) · Biotechnology and Bioprocessing · doi:10.31579/2766-2314/042 · Cited by 3
Mechanism of the CRISPR/Cas9 System Specificity in Genome Editing
Kulishova, L. M., Vokhtantsev, I. P., Kim, D. V., & Zharkov, D. O. (2023) · Molecular Biology · doi:10.31857/s0026898423020155 · Cited by 3
Gomaa, A. E., Zhang, C., Yang, Z., Shang, L., Jiang, S., Deng, Z., Zhan, Y., Lü, W., Lin, M., & Yan, Y. (2017) · AMB Express · doi:10.1186/s13568-017-0425-y · Cited by 3
Cheng, C., Wong, T. H. Y., Yung, Y., Chan, N. C. N., & Ng, M. H. L. (2019) · Journal of Visualized Experiments · doi:10.3791/60130 · Cited by 3
Evolutionary Timeline of Genetic Delivery and Gene Therapy
Holl, N. J., Lee, H., & Huang, Y. (2020) · Current Gene Therapy · doi:10.2174/1566523220666201208092517 · Cited by 3
Tissue-Specific Cell Penetrating Peptides for Targeted Delivery of Small Interfering RNAs
Lopuszynski, J. B., Agrawal, V., & Zahid, M. (2022) · Medical Research Archives · doi:10.18103/mra.v10i8.2998 · Cited by 3
CRISPR/Cas9-Facilitated Chromosome Engineering to Model Human Chromosomal Alterations
Xing, Z., Li, Y., Pao, A., Kaas, G. A., & Yu, Y. (2018) · InTech eBooks · doi:10.5772/intechopen.70897 · Cited by 3
Manufacturing and Delivering Genome-Editing Proteins
Liu, J., Liang, Y.-J., Ren, P.-L., & Gaj, T. (2018) · Methods in molecular biology · doi:10.1007/978-1-4939-8799-3_19 · Cited by 3
Methods for CPP Functionalization
Langel, Ü. (2019) · Springer eBooks · doi:10.1007/978-981-13-8747-0_3 · Cited by 3
CRISPR/Cas9 Age in Genome Editing and Leukemia Applications
Gümüş, N., & Kaymaz, B. T. (2018) · Kafkas Journal of Medical Sciences · doi:10.5505/kjms.2018.94715 · Cited by 3
[Development of CRISPR technology and its application in bone and cartilage tissue engineering].
Chen, G., Cheng, D., & Chen, B. (2019) · Europe PMC (PubMed Central) · doi:10.12122/j.issn.1673-4254.2019.12.19 · Cited by 3
βTrCP1 promotes SLC35F2 protein ubiquitination and inhibits cancer progression in HeLa cells
Colaco, J. C., Chandrasekaran, A. P., Karapurkar, J. K., Birappa, G., Rajkumar, S., Gowda, D. A. A., Suresh, B., Lee, J., Singh, V., Hong, S., et al. (2023) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2023.09.095 · Cited by 2
Self-citationShum, C., Han, S. Y., Thiruvahindrapuram, B., Wang, Z., de Rijke, J., Zhang, B., Sundberg, M., Chen, C.-D., Buttermore, E. D., Makhortova, N. R., et al. (2023) · Computational and Structural Biotechnology Journal · doi:10.1016/j.csbj.2023.12.036 · Cited by 2
Combating bone marrow failure with polymer materials
Koch, K. C., Jadon, N., Thesmar, I., Tew, G. N., & Minter, L. M. (2024) · Frontiers in Immunology · doi:10.3389/fimmu.2024.1396486 · Cited by 2
Decoding Advances in Psychiatric Genetics
Heckenast, J. R., Wilkinson, L. S., & Jones, M. W. (2015) · Advances in genetics · doi:10.1016/bs.adgen.2015.09.001 · Cited by 2
Gene Editing in Regenerative Medicine
Fang, Y., Chen, X., & Godbey, W. T. (2018) · Elsevier eBooks · doi:10.1016/b978-0-12-809880-6.00042-4 · Cited by 2
Induced Mutagenesis in Date Palm (Phoenix dactylifera L.) Breeding
Sattar, M. N., Iqbal, Z., Naqqash, M. N., Jain, S. M., & Al-Khayri, J. M. (2021) · Compendium of plant genomes · doi:10.1007/978-3-030-73750-4_7 · Cited by 2
An Introduction to Genome Editing Techniques
Munawar, N., & Ahmad, A. (2022) · Springer eBooks · doi:10.1007/978-981-16-6305-5_1 · Cited by 2
CRISPR/Cas9 Editing in Induced Pluripotent Stem Cells: A Way Forward for Treating Cystic Fibrosis?
Quiroz, E. J., & Ryan, A. L. (2019) · Springer eBooks · doi:10.1007/978-3-030-29403-8_9 · Cited by 2
CRISPR-Cas9 system “a mighty player in cancer therapy”
Manvati, S., & Dhar, P. K. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-818140-9.00008-8 · Cited by 2
Constraining TAT Peptide by γPNA Hairpin for Enhanced Cellular Delivery of Biomolecules
Thennakoon, S. K. S., Postema, R. M., & Tan, X. (2021) · Methods in molecular biology · doi:10.1007/978-1-0716-1617-8_20 · Cited by 2
Reengineering of the CRISPR/Cas System
Khan, Z., Sattar, T., Siddique, M., Ali, Z., Khan, A. A., Aftab, S. O., Ghouri, M. Z., Sultan, Q., Gulzar, N., & Ahmad, F. (2022) · Springer eBooks · doi:10.1007/978-981-16-6305-5_5 · Cited by 2
Slaughter, C. A. (2014) · Journal of Biomolecular Techniques JBT · doi:10.7171/jbt.14-2503-002 · Cited by 2
Singh, D. (2024) · Current Drug Therapy · doi:10.2174/0115748855284955240304053519 · Cited by 1
Qi, S., Wang, Y., Liu, Z., Wu, S., Zhao, Y., Li, Y., Deng, S., Yu, K., & Lian, Z. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms26031331 · Cited by 1
Functional peptide-assisted delivery of CRISPR/Cas9: recent progress on gene editing
Cho, H. M., Ryu, Y. C., Cho, M. K., & Hwang, B. H. (2026) · Biomaterials Science · doi:10.1039/d6bm00231e · Cited by 1
Lummerstorfer, M., Xue, Z., Zheng, D., König, L., Brunner, L., & Lächelt, U. (2026) · European Journal of Pharmaceutical Sciences · doi:10.1016/j.ejps.2026.107596 · Cited by 1
Ivleva, A., Demchenko, A. G., & Smirnikhina, S. (2026) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2026.1855907 · Cited by 1
The future of pediatric gene therapy: CRISPR-Cas9, AI, and personalized medicine
Pan, Y., Ding, J., Wang, W., Xu, D., Fan, J., & Zhang, W. (2026) · Pediatric Research · doi:10.1038/s41390-026-05270-3 · Cited by 2
Editing Cultured Human Cells: From Cell Lines to iPS Cells
Li, H. L., & Hotta, A. (2014) · Springer eBooks · doi:10.1007/978-4-431-55227-7_3 · Cited by 1
Programmable delivery of macromolecules using high frequency ultrasound
Yoon, S., Kim, M., Shung, K. K., & Wang, Y. (2015) · IEEE International Ultrasonics Symposium · doi:10.1109/ultsym.2015.0031 · Cited by 1
Cellular Therapies: Gene Editing and Next-Gen CAR T Cells
Cradick, T. J. (2016) · Springer eBooks · doi:10.1007/978-3-319-29827-6_9 · Cited by 1
Nie, Y., Cong, P., Liu, X., Wang, M., Chen, Y., & He, Z. (2016) · Biotechnology Letters · doi:10.1007/s10529-016-2259-0 · Cited by 1
Trendbericht Biochemie 2017: Zellpenetration
Avrutina, O., Kolmar, H., & Empting, M. (2018) · Nachrichten aus der Chemie · doi:10.1002/nadc.20184071749 · Cited by 1
Worgall, S., & Crystal, R. G. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-818422-6.00029-0 · Cited by 1
CRISPR: A Promising Tool for Cancer Therapy
Mohammad‐Rafiei, F., Safdarian, E., Adel, B., Vandchali, N. R., Navashenaq, J. G., & Hayat, S. M. G. (2022) · Current Molecular Medicine · doi:10.2174/1566524022666220624111311 · Cited by 1
Genome Editing: A Review of the Challenges and Approaches
Sharma, D., Kaur, H., Kapoor, H. K., Sharma, R., Kaur, H., & Kyum, M. (2022) · Springer eBooks · doi:10.1007/978-3-031-08072-2_4 · Cited by 1
Additional Gene Therapeutic Platforms
Langel, Ü. (2023) · Springer eBooks · doi:10.1007/978-3-031-38731-9_6 · Cited by 1
Reagents and their delivery systems in CRISPR/Cas
Khan, S., Zafar, H., & Ahmad, A. (2024) · Elsevier eBooks · doi:10.1016/b978-0-443-18444-4.00002-8 · Cited by 1
Foss, D. V., & Norris, A. L. (2024) · Elsevier eBooks · doi:10.1016/b978-0-12-817218-6.00011-5 · Cited by 2
Genome engineering in maize using CRISPR/CAS9 system
Rafique, K., Gul, A., Ahmad, N., & Mushtaq, N. (2024) · Elsevier eBooks · doi:10.1016/b978-0-443-26614-0.00002-3 · Cited by 1
Adapting and Testing ReMOT Control for Expanded CRISPR-Era Genome Functions in Non-model Insects
Dave, S., Patel, C., Heu, C. C., & Macias, V. M. (2025) · Methods in molecular biology · doi:10.1007/978-1-0716-4583-3_16 · Cited by 1
Cell-penetrating peptides in nanodelivery of nucleic acids and drugs
Özyurt, C., Uğurlu, Ö., & Evran, S. (2018) · Elsevier eBooks · doi:10.1016/b978-0-12-813665-2.00002-8 · Cited by 1
Geny, S., Hosseini, E. S., Concordet, J., & Giovannangeli, C. (2019) · Royal Society of Chemistry eBooks · doi:10.1039/9781788015714-00400 · Cited by 1
INSIGHT INTO CRISPR SYSTEM IN EUKARYOTIC MICROALGAE, A REVIEW
Bilal, I., Butnariu, M., & Bilal, E. (2019) · EurasianUnionofScientists · doi:10.31618/esu.2413-9335.2019.3.59.37-48 · Cited by 1
Liposome-Templated Hydrogel Nanoparticles for Targeted Delivery of CRISPR/Cas9 to Brain Tumors
Chen, Z., Chen, A. T., & Zhou, J. (2020) · Neuromethods · doi:10.1007/978-1-0716-1052-7_10 · Cited by 1
Wu, H., Zhang, Z., Zhu, K., Wang, Y., Ke, F., Zhang, F., Ying, Z., Zhang, Y., & Feng, H. (2022) · In Vitro Cellular & Developmental Biology - Plant · doi:10.1007/s11627-021-10221-0 · Cited by 1
Delivery Methods for CRISPR/Cas Reagents
Ashraf, S., Munawar, N., Zahoor, M. K., Jamil, A., Hammad, M., Ghaffar, A., & Ahmad, A. (2022) · Springer eBooks · doi:10.1007/978-981-16-6305-5_4 · Cited by 1
Chen, T., Zhang, X., & Wu, Q. (2021) · Quantitative Biology · doi:10.15302/j-qb-021-0253 · Cited by 1
Au, V., Li-Leger, E., Raymant, G., Flibotte, S., Chen, G., Martin, K., Fernando, L., Doell, C., Rosell, F. I., Wang, S., et al. (2018) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/359588 · Cited by 1
Cell Penetrating Peptides Mediated DNA-Free Transfection in Arabidopsis and Chinese CabbagePreprint
Wu, H., Zhang, Z., Zhu, K., Ke, F., Zhang, F., Zhang, Y., & Feng, H. (2021) · Research Square · doi:10.21203/rs.3.rs-141818/v1 · Cited by 1
Cas9-mediated gene disruption in tetraploid Giardia intestinalisPreprint
Horáčková, V., Voleman, L., Hagen, K. D., Petrů, M., Vinopalová, M., Weisz, F., Janowicz, N., Marková, L., Motyčková, A., Tůmová, P., et al. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.04.21.440745 · Cited by 1
Gene Delivery Technologies for Efficient Genome Editing: Applications in Gene Therapy
Martín, F., Sánchez-Hernández, S., Gutiérrez-Guerrero, A., & Benabdellah, K. (2016) · InTech eBooks · doi:10.5772/62776 · Cited by 1
Engineering the zinc finger recombinase for use in targeted genomic editing
Kentner, J. L. (2015) · Enlighten: Theses (The University of Glasgow)
Heroes of peer review: Hyongbum (Henry) Kim
Kim, S. (2016) · Genome biology · doi:10.1186/s13059-016-1067-0
Designer Effectors for Editing and Regulating Complex Genomes
Müller, M., Mlambo, T., & Mussolino, C. (2017) · Springer eBooks · doi:10.1007/978-3-319-53457-2_7
The fourth annual BRDS on genome editing and silencing for precision medicines
Chaudhary, A., Bhattarai, R. S., & Mahato, R. I. (2017) · Drug Delivery and Translational Research · doi:10.1007/s13346-017-0457-5
秦, 舟., 门, 可., 徐, 珽., 何, 治., 魏, 于., & 杨, 阳. (2017) · Scientia Sinica Vitae · doi:10.1360/n052017-00202
Towards best-practice approaches for CRISPR/Cas9 gene engineeringPreprint
Campenhout, C. V., Cabochette, P., Veillard, A.-C., Laczik, M., Zelisko-Schmidt, A., Sabatel, C., Dhainaut, M., Vanhollebeke, B., Gueydan, C., & Kruys, V. (2018) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/469544
Current status and future prospects of virus-based gene medicine
Sakurai, F. (2019) · Drug Delivery System · doi:10.2745/dds.34.99
Metadata
- Authors
- Ramakrishna, S., Kwaku Dad, A.-B., Beloor, J., Gopalappa, R., Lee, S.-K., & Kim, H.
- Affiliations
- Hanyang University (KR)
- Venue
- Genome Research
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2014-04-02
- Volume
- 24
- Issue
- 6
- Pages
- 1020-1027
- ISSN
- 1088-9051 (ISSN-L)
- PMC
- PMC4032848
- OpenAlex
- W2103609218
- Cited by
- 655
- References
- 45
- Citations by year
- 2026: 16 · 2025: 31 · 2024: 41 · 2023: 46 · 2022: 64 · 2021: 76 · 2020: 66 · 2019: 60 · 2018: 77 · 2017: 60 · 2016: 56 · 2015: 50 · 2014: 11
- Type
- posted content
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc
- Locations
- Genome Research (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- HighWire Press Open Archive (submittedVersion)
- Funders
- National Research Foundation
- Hanyang University
- Yonsei University
- Korea Basic Science Institute
- National Research Foundation of Korea (2013M3A9B4076544, 2011‐0019357)
- Korea Advanced Institute of Science and Technology
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Keywords
- CRISPR/Cas9, cell-penetrating peptides, gene disruption, genome editing, guide RNA delivery, off-target mutations, embryonic stem cells, human cell lines
- MeSH terms
- RNA, Guide, CRISPR-Cas Systems, Humans, Transfection, Genome, Human, Mutagenesis, Site-Directed, RNA Editing, RNA, Guide, Kinetoplastida, Cell Line, Tumor, Nanoparticles, HEK293 Cells, Cell-Penetrating Peptides, CRISPR-Cas Systems
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
