PreprintCited by 51Neurobiology and Insect Physiology ResearchMemory and Neural MechanismsNeuroscience and Neuropharmacology Research

Second-order conditioning in Drosophila

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Tabone, Christopher J., de Belle, J. Steven·2011·Learning & Memory

Abstract

Associative conditioning in Drosophila melanogaster has been well documented for several decades. However, most studies report only simple associations of conditioned stimuli (CS, e.g., odor) with unconditioned stimuli (US, e.g., electric shock) to measure learning or establish memory. Here we describe a straightforward second-order conditioning (SOC) protocol that further demonstrates the flexibility of fly behavior. In SOC, a previously conditioned stimulus (CS1) is used as reinforcement for a second conditioned stimulus (CS2) in associative learning. This higher-order context presents an opportunity for reassessing the roles of known learning and memory genes and neuronal networks in a new behavioral paradigm.

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  1. Pavlov IP. 1927. Conditioned reflexes. Oxford Press, Oxford, UK.

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Authors
Tabone, C. J., & de Belle, J. S.
Affiliations
University of Nevada, Las Vegas (US)
Venue
Learning & Memory
Published
2011-03-25
Volume
18
Issue
4
Pages
250-253
ISSN
  • 1072-0502 (ISSN-L)
  • 1549-5485
OpenAlex
W2102183435
Cited by
51
References
84
Citations by year
2026: 2 · 2025: 6 · 2024: 9 · 2023: 5 · 2022: 4 · 2021: 3 · 2020: 5 · 2019: 3 · 2018: 1 · 2017: 3 · 2016: 2 · 2015: 1 · 2014: 3 · 2013: 4
Type
posted content
Language
en
Open access
Locations
  • Learning & Memory (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Science Foundation (0237395)
  • University of Nevada, Las Vegas
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
second-order conditioning, Drosophila melanogaster, associative learning, olfactory conditioning, electric shock, neuronal networks, behavioral flexibility
MeSH terms
Analysis of Variance, Animals, Behavior, Animal, Conditioning, Classical, Discrimination Learning, Drosophila, Electric Stimulation, Extinction, Psychological, Odorants, Reinforcement, Psychology, Smell, Maze Learning

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