Induction of a dominant negative CREB transgene specifically blocks long-term memory in Drosophila
Abstract
Consolidated memory after olfactory learning in Drosophila consists of two components, a cycloheximide-sensitive, long-term memory (LTM) and a cycloheximide-insensitive, anesthesia-resistant memory (ARM). Using an inducible transgene that expresses a dominant negative member of the fly CREB family, LTM was specifically and completely blocked only after induction, while ARM and learning were unaffected. These results suggest that LTM formation requires de novo gene expression probably mediated by CREB family genes.
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Previous efforts to find neurobiological explanations for the advantages of distributed practice obtained evidence that spaced trials can offset memory defects in mice lacking two variants of the transcription factor CREB.
“Previous efforts to find neurobiological explanations for the advantages of distributed practice obtained evidence that spaced trials can offset memory defects in mice lacking two variants of the transcription factor CREB (31, 32).”
From Synaptic evidence for the efficacy of spaced learning (2012) · cites this paper as [32]
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- Authors
- Yin, J. C. P., Wallach, J. S., Del Vecchio, M., Wilder, E. L., Zhou, H., Quinn, W. G., & Tully, T.
- Affiliations
- Cold Spring Harbor Laboratory (US)
- Massachusetts Institute of Technology (US)
- Howard Hughes Medical Institute (US)
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1994-10
- Volume
- 79
- Issue
- 1
- Pages
- 49-58
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2076782084
- Cited by
- 959
- References
- 48
- Citations by year
- 2026: 6 · 2025: 11 · 2024: 10 · 2023: 21 · 2022: 16 · 2021: 19 · 2020: 14 · 2019: 25 · 2018: 19 · 2017: 32 · 2016: 27 · 2015: 28 · 2014: 25 · 2013: 41 · 2012: 41
- FWCI
- 13.95
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Cold Spring Harbor Laboratory Institutional Repository (Cold Spring Harbor Laboratory) (acceptedVersion)
- Funders
- National Institutes of Health (HD32245, NS25621)
- National Cancer Institute
- Cold Spring Harbor Laboratory
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- long-term memory, olfactory learning, dominant-negative CREB, de novo gene expression, memory consolidation, cycloheximide sensitivity, Drosophila
- MeSH terms
- Amino Acid Sequence, Animals, Conditioning, Classical, Drosophila, Genes, Dominant, Hot Temperature, Memory, Molecular Sequence Data, RNA, Messenger, Gene Expression, Genes, Insect, Cyclic AMP Response Element-Binding Protein, Animals, Genetically Modified
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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