The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Short-Range Spectroscopic Ruler Based on a Single-Molecule Optical Switch
Open accessAbstract
We demonstrate a novel all-optical switch consisting of two molecules: a primary fluorophore (Cy5) that can be switched between a fluorescent and a dark state by light of different wavelengths, and a secondary chromophore (Cy3) that facilitates switching. The interaction between the two molecules exhibits a distance dependence much steeper than that of conventional Fo rster resonance energy transfer. This enables the switch to act as a ruler with the capability to probe distances difficult to access by other spectroscopic methods, thus presenting a new tool for the study of biomolecules at the single-molecule level.
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Cites
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Myosin V Walks Hand-Over-Hand: Single Fluorophore Imaging with 1.5-nm Localization
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Single-Molecule Enzymatic Dynamics
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Ten years of tension: single-molecule DNA mechanics
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Probing the free-energy surface for protein folding with single-molecule fluorescence spectroscopy
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Protein Conformational Dynamics Probed by Single-Molecule Electron Transfer
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A Single-Molecule Study of RNA Catalysis and Folding
Zhuang, X., Bartley, L. E., Babcock, H. P., Russell, R., Ha, T., Herschlag, D., & Chu, S. (2000) · Science · doi:10.1126/science.288.5473.2048 · Cited by 732
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Cited by
Super-resolution fluorescence microscopy
Huang, B., Bates, M., & Zhuang, X. (2009) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.77.061906.092014 · Cited by 1,657
Self-citationMulticolor Super-Resolution Imaging with Photo-Switchable Fluorescent Probes
Bates, M., Huang, B., Dempsey, G. T., & Zhuang, X. (2007) · Science · doi:10.1126/science.1146598 · Cited by 1,504
Self-citationEvaluation of fluorophores for optimal performance in localization-based super-resolution imaging
Dempsey, G. T., Vaughan, J. C., Chen, K. H., Bates, M., & Zhuang, X. (2011) · Nature Methods · doi:10.1038/nmeth.1768 · Cited by 1,369
Self-citationFluorescent probes for super-resolution imaging in living cells
Fernández-Suárez, M., & Ting, A. Y. (2008) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2531 · Cited by 1,306
A simple, versatile method for GFP-based super-resolution microscopy via nanobodies
Ries, J., Kaplan, C., Platonova, E., Eghlidi, H., & Ewers, H. (2012) · Nature Methods · doi:10.1038/nmeth.1991 · Cited by 571
Metadata
- Authors
- Bates, M., Blosser, T. R., & Zhuang, X.
- Venue
- Physical Review Letters
- Publisher
- American Physical Society (APS)
- Published
- 2005-03-15
- Volume
- 94
- Issue
- 10
- Pages
- 108101-108101
- ISSN
- 0031-9007 (ISSN-L)
- 1079-7114
- OpenAlex
- W2001022761
- Cited by
- 364
- References
- 24
- FWCI
- 9.95
- Type
- journal article
- Language
- en
- Open access
- green
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