The DOI matches its Crossref record; 52 of the 52 references it cites were found in a registry or an index.
Patterns of Sequence Loss and Cytosine Methylation within a Population of Newly Resynthesized Brassica napus Allopolyploids
Open accessAbstract
Allopolyploid formation requires the adaptation of two nuclear genomes within a single cytoplasm, which may involve programmed genetic and epigenetic changes during the initial generations following genome fusion. To study the dynamics of genome change, we synthesized 49 isogenic Brassica napus allopolyploids and surveyed them with 76 restriction fragment length polymorphism (RFLP) probes and 30 simple sequence repeat (SSR) primer pairs. Here, we report on the types and distribution of genetic and epigenetic changes within the S1 genotypes. We found that insertion/deletion (indel) events were rare, but not random. Of the 57,710 (54,383 RFLP and 3,327 SSR) parental fragments expected among the amphidiploids, we observed 56,676 or 99.9%. Three loci derived from Brassica rapa had indels, and one indel occurred repeatedly across 29% (14/49) of the lines. Loss of one parental fragment was due to the 400-bp reduction of a guanine-adenine dinucleotide repeat-rich sequence. In contrast to the 4% (3/76) RFLP probes that detected indels, 48% (35/73) detected changes in the CpG methylation status between parental genomes and the S1 lines. Some loci were far more likely than others to undergo epigenetic change, but the number of methylation changes within each synthetic polyploid was remarkably similar to others. Clear de novo methylation occurred at a much higher frequency than de novo demethylation within allopolyploid sequences derived from B. rapa. Our results suggest that there is little genetic change in the S0 generation of resynthesized B. napus polyploids. In contrast, DNA methylation was altered extensively in a pattern that indicates tight regulation of epigenetic changes.
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Cites
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Self-citationPolyploidy and Novelty in Flowering Plants
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Requirement of CHROMOMETHYLASE3 for Maintenance of CpXpG Methylation
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Understanding mechanisms of novel gene expression in polyploids
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Cited by
Evolutionary Genetics of Genome Merger and Doubling in Plants
Doyle, J. J., Flagel, L. E., Paterson, A. H., Rapp, R. A., Soltis, D. E., Soltis, P. S., & Wendel, J. F. (2008) · Annual Review of Genetics · doi:10.1146/annurev.genet.42.110807.091524 · Cited by 684
Genomic Changes in Resynthesized Brassica napus and Their Effect on Gene Expression and Phenotype
Gaeta, R. T., Pires, J. C., Iniguez-Luy, F., Leon, E., & Osborn, T. C. (2007) · The Plant Cell · doi:10.1105/tpc.107.054346 · Cited by 629
Self-citationDoubling down on genomes: Polyploidy and crop plants
Renny‐Byfield, S., & Wendel, J. F. (2014) · American Journal of Botany · doi:10.3732/ajb.1400119 · Cited by 406
Chester, M., Gallagher, J. P., Symonds, V. V., Cruz da Silva, A. V., Mavrodiev, E. V., Leitch, A. R., Soltis, P. S., & Soltis, D. E. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1112041109 · Cited by 383
Homoeologous recombination in allopolyploids: the polyploid ratchet
Gaeta, R. T., & Chris Pires, J. (2010) · New Phytologist · doi:10.1111/j.1469-8137.2009.03089.x · Cited by 333
Metadata
- Authors
- Lukens, L. N., Pires, J. C., Leon, E., Vogelzang, R., Oslach, L., & Osborn, T.
- Venue
- Plant Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2005-12-23
- Volume
- 140
- Issue
- 1
- Pages
- 336-348
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- OpenAlex
- W2140052305
- Cited by
- 263
- References
- 52
- FWCI
- 13.38
- Type
- journal article
- Language
- en
- Open access
- green
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- Lukens, L. N., Pires, J. C., Leon, E., Vogelzang, R., Oslach, L., & Osborn, T. (2006). Patterns of Sequence Loss and Cytosine Methylation within a Population of Newly Resynthesized Brassica napus Allopolyploids. Plant Physiology. https://doi.org/10.1104/pp.105.066308
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