The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
CRISPR-Cas9 Targeted Mutagenesis Leads to Simultaneous Modification of Different Homoeologous Gene Copies in Polyploid Oilseed Rape (Brassica napus)
Open accessAbstract
In polyploid species, altering a trait by random mutagenesis is highly inefficient due to gene redundancy. We have stably transformed tetraploid oilseed rape (Brassica napus) with a CRISPR-Cas9 construct targeting two ALCATRAZ (ALC) homoeologs. ALC is involved in valve margin development and, thus, contributes to seed shattering from mature fruits. Knocking out ALC would increase shatter resistance to avoid seed loss during mechanical harvest. We obtained a transgenic T1 plant with four alc mutant alleles by the use of a single target sequence. All mutations were stably inherited to the T2 progeny. The T2 generation was devoid of any wild-type alleles, proving that the underlying T1 was a nonchimeric double heterozygote. T-DNA and ALC loci were not linked, as indicated by random segregation in the T2 generation. Hence, we could select double mutants lacking the T-DNA already in the first offspring generation. However, whole-genome sequencing data revealed at least five independent insertions of vector backbone sequences. We did not detect any off-target effects in two genome regions homologous to the target sequence. The simultaneous alteration of multiple homoeologs by CRISPR-Cas9 mutagenesis without any background mutations will offer new opportunities for using mutant genotypes in rapeseed breeding.
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CRISPR/Cas system: recent advances and future prospects for genome editing
Manghwar, H., Lindsey, K., Zhang, X., & Jin, S. (2019) · Trends in Plant Science · doi:10.1016/j.tplants.2019.09.006 · Cited by 529
The emerging and uncultivated potential of CRISPR technology in plant science
Zhang, Y., Malzahn, A. A., Sretenovic, S., & Qi, Y. (2019) · Nature Plants · doi:10.1038/s41477-019-0461-5 · Cited by 394
The CRISPR/Cas9 system and its applications in crop genome editing
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Detection and Identification of Genome Editing in Plants: Challenges and Opportunities
Grohmann, L., Keilwagen, J., Duensing, N., Dagand, E., Hartung, F., Wilhelm, R., Bendiek, J., & Sprink, T. (2019) · Frontiers in Plant Science · doi:10.3389/fpls.2019.00236 · Cited by 135
Metadata
- Authors
- Braatz, J., Harloff, H.-J., Mascher, M., Stein, N., Himmelbach, A., & Jung, C.
- Venue
- Plant Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2017-04-18
- Volume
- 174
- Issue
- 2
- Pages
- 935-942
- ISSN
- 0032-0889 (ISSN-L)
- 1532-2548
- OpenAlex
- W2607339918
- Cited by
- 265
- References
- 29
- FWCI
- 10.09
- Type
- journal article
- Language
- en
- Open access
- green
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- Braatz, J., Harloff, H.-J., Mascher, M., Stein, N., Himmelbach, A., & Jung, C. (2017). CRISPR-Cas9 Targeted Mutagenesis Leads to Simultaneous Modification of Different Homoeologous Gene Copies in Polyploid Oilseed Rape (Brassica napus). Plant Physiology. https://doi.org/10.1104/pp.17.00426
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