CoChat
Sign Up
Cited by 289Plant Molecular Biology ResearchPlant Parasitism and ResistanceLight effects on plants

The Branching Gene RAMOSUS1 Mediates Interactions among Two Novel Signals and Auxin in Pea

Open access
Foo, Eloise, Bullier, Erika, Goussot, Magali, Foucher, Fabrice, Rameau, Catherine, Beveridge, Christine Anne·2005·The Plant Cell
DOI 10.1105/tpc.104.026716PMC PMC548819
Open this paper in CoChat — free accountOpen PDF

Abstract

In Pisum sativum, the RAMOSUS genes RMS1, RMS2, and RMS5 regulate shoot branching via physiologically defined mobile signals. RMS1 is most likely a carotenoid cleavage enzyme and acts with RMS5 to control levels of an as yet unidentified mobile branching inhibitor required for auxin inhibition of branching. Our work provides molecular, genetic, and physiological evidence that RMS1 plays a central role in a shoot-to-root-to-shoot feedback system that regulates shoot branching in pea. Indole-3-acetic acid (IAA) positively regulates RMS1 transcript level, a potentially important mechanism for regulation of shoot branching by IAA. In addition, RMS1 transcript levels are dramatically elevated in rms3, rms4, and rms5 plants, which do not contain elevated IAA levels. This degree of upregulation of RMS1 expression cannot be achieved in wild-type plants by exogenous IAA application. Grafting studies indicate that an IAA-independent mobile feedback signal contributes to the elevated RMS1 transcript levels in rms4 plants. Therefore, the long-distance signaling network controlling branching in pea involves IAA, the RMS1 inhibitor, and an IAA-independent feedback signal. Consistent with physiological studies that predict an interaction between RMS2 and RMS1, rms2 mutations appear to disrupt this IAA-independent regulation of RMS1 expression.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1105/tpc.104.026716

Cites

8 of 8 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • The primary mechanism of strigolactone function is expected to be conserved across plant species.

    “One would expect the primary mechanism of strigolactone function to be conserved across plant species, especially due to high conservation of the biosynthetic pathway and its regulation by auxin (Fig. 6; Sorefan et al., 2003;Bainbridge et al., 2005;Foo et al., 2005;Zou et al., 2006;Arite et al., 2007;Gomez-Roldan et al., 2008;Umehara et al., 2008).”

    From Strigolactone Acts Downstream of Auxin to Regulate Bud Outgrowth in Pea and Arabidopsis (2009) · cites this paper as Foo et al., 2005

    The Introduction (page not supplied) identifies homologous branching-related genes in pea, Arabidopsis, and petunia, supporting a shared biosynthetic component. But “Auxin Regulation of RMS1” (page not supplied) cautions that auxin regulation may differ between pea and Arabidopsis. The paper therefore supports part of the rationale for expecting conservation, not conservation of the primary mechanism across plant species. (LLM, conf=0.84)

    Experimental — accuracy not yet measured

  • It was suggested that the feedback is regulated by the absence of perception of SL, as various mutants unable to produce or respond to SL have reduced export of X-CK from roots and increased expression of SL biosynthesis genes.

    “It was instead suggested to be regulated by the absence of perception of SL (Foo et al., 2005;Dun et al., 2009), as various mutants unable to produce (rms1 and rms5) or to respond to (rms3 and rms4) SL have reduced export of X-CK from the roots (Beveridge et al., 1997a;Morris et al., 2001;Foo et al., 2007) and increased expression of SL biosynthesis genes (Foo et al., 2005;Johnson et al., 2006;Ari”

    From The Pea TCP Transcription Factor PsBRC1 Acts Downstream of Strigolactones to Control Shoot Branching (2012) · cites this paper as Foo et al., 2005

    In “RMS1 Expression Is Feedback Regulated” (page not supplied), the paper reports elevated RMS1 expression in rms1, rms3, rms4, and rms5 mutants and suggests that feedback may respond to an actual or perceived reduction in a branching inhibitor. The Introduction (page not supplied) also reports reduced root-to-shoot cytokinin export in those mutants. The paper does not identify the inhibitor as strigolactone (SL) or establish absence of SL perception as the specific cause of the feedback. (LLM, conf=0.86)

    Experimental — accuracy not yet measured

  • Auxin controls CK and SL biosynthesis, and both hormones act downstream of auxin and converge to BRC1 to control bud outgrowth.

    “In this model, auxin controls CK and SL biosynthesis (Foo et al., 2005; Shimizu-Sato et al., 2009). Both hormones act downstream of auxin and converge to BRC1 to control bud outgrowth.”

    From Multiple pathways regulate shoot branching (2015) · cites this paper as Foo et al., 2005

    The 'Auxin Regulation of RMS1 Expression' section (page unavailable) reports that IAA maintains or increases expression of RMS1, which the Introduction links to synthesis of a branching inhibitor. The paper discusses cytokinin export from roots, not auxin control of cytokinin biosynthesis; it also does not identify the inhibitor as strigolactone. The citation is attached to the first sentence, so the subsequent BRC1 statement is not attributed to this source. (LLM, conf=0.86)

    Experimental — accuracy not yet measured

  • Decapitation triggers down-regulation of SL biosynthesis gene CCD8 transcript levels, most likely reducing SL biosynthesis.

    “Decapitation triggers down-regulation of SL biosynthesis gene CCD8 transcript levels (Foo et al., 2005), most likely resulting in reduced SL biosynthesis.”

    From Shaping plant architecture (2015) · cites this paper as Foo et al., 2005

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 3

  1. Booker, J., Sieberer, T., Wright, W., Williamson, L., Willett, B., Stirnberg, P., Turnbull, C., Srinivasan, M., Goddard, P., and Leyser, O. (2005). The MAX1 gene of Arabidopsis encodes a puta- tive cytochrome P450 that acts downstream of MAX3 and MAX4 in the synthesis of a novel branch-inhibiting hormone. Dev. Cell, in press.
  2. Kreig, P.A. (1996). A Laboratory Guide to RNA Isolation, Analysis and Synthesis. (New York: John Wiley & Sons).
  3. Snowden, K.C., Simkin, A.J., Janssen, B.J., Templeton, K.R., Loucas, H.M., Simons, J.L., Karunairetnam, S., Gleave, A.P., Clark, D.G., and Klee, H.J. (2005). The Dad1/PhCCD8 gene affects branch production and has a role in leaf senescence, root growth, and flower development. Plant Cell, in press.

Cited by

Sorted by citations

Authors
Foo, E., Bullier, E., Goussot, M., Foucher, F., Rameau, C., & Beveridge, C. A.
Affiliations
  • Australian Research Council (AU)
  • Institut Jean-Pierre Bourgin (FR)
ORCID
Venue
The Plant Cell
Publisher
Oxford University Press (OUP)
Published
2005-01-19
Volume
17
Issue
2
Pages
464-474
ISSN
  • 1040-4651 (ISSN-L)
  • 1532-298X
DOI
10.1105/tpc.104.026716
PMC
PMC548819
OpenAlex
W2122404205
Cited by
289
References
8
Citations by year
2026: 5 · 2025: 7 · 2024: 15 · 2023: 9 · 2022: 8 · 2021: 15 · 2020: 10 · 2019: 14 · 2018: 9 · 2017: 17 · 2016: 9 · 2015: 19 · 2014: 16 · 2013: 8 · 2012: 23
FWCI
22.05
Type
journal article
Language
en
Open access
Licence
aspb.org/publications/aspb-journals/open-articles (vor, 378 d)
Locations
  • The Plant Cell (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • eCite Digital Repository (University of Tasmania) (submittedVersion)
  • HAL (submittedVersion)
  • Figshare (submittedVersion, cc-by)
Funders
Australian Research Council
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Parasitism and Resistance
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
Keywords
shoot branching, auxin, indole-3-acetic acid, carotenoid cleavage enzymes, long-distance signaling, pea, Pisum sativum, gene expression regulation, grafting
MeSH terms
Alleles, Chromosome Mapping, Indoleacetic Acids, Models, Biological, Molecular Sequence Data, Oxygenases, Phenotype, Signal Transduction, Up-Regulation, Gene Expression Regulation, Plant, Plant Shoots, Pisum sativum, Gene Expression Profiling

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review