The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
New tools for the analysis and validation of cryo-EM maps and atomic models
Open accessAbstract
Recent advances in the field of electron cryomicroscopy (cryo-EM) have resulted in a rapidly increasing number of atomic models of biomacromolecules that have been solved using this technique and deposited in the Protein Data Bank and the Electron Microscopy Data Bank. Similar to macromolecular crystallography, validation tools for these models and maps are required. While some of these validation tools may be borrowed from crystallography, new methods specifically designed for cryo-EM validation are required. Here, new computational methods and tools implemented in PHENIX are discussed, including d 99 to estimate resolution, phenix.auto_sharpen to improve maps and phenix.mtriage to analyze cryo-EM maps. It is suggested that cryo-EM half-maps and masks should be deposited to facilitate the evaluation and validation of cryo-EM-derived atomic models and maps. The application of these tools to deposited cryo-EM atomic models and maps is also presented.
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PHENIX : a comprehensive Python-based system for macromolecular structure solution
Adams, P. D., Afonine, P. V., Bunkóczi, G., Chen, V. B., Davis, I. W., Echols, N., Headd, J. J., Hung, L.-W., Kapral, G. J., Grosse-Kunstleve, R. W., et al. (2010) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444909052925 · Cited by 23,716
Self-citationMolProbity : all-atom structure validation for macromolecular crystallography
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Linking Crystallographic Model and Data Quality
Karplus, P. A., & Diederichs, K. (2012) · Science · doi:10.1126/science.1218231 · Cited by 1,781
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A real-space refinement procedure for proteins
Diamond, R. (1971) · Acta Crystallographica Section A · doi:10.1107/s0567739471000986 · Cited by 236
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Metadata
- Authors
- Afonine, P. V., Klaholz, B. P., Moriarty, N. W., Poon, B. K., Sobolev, O. V., Terwilliger, T. C., Adams, P. D., & Urzhumtsev, A.
- Venue
- Acta Crystallographica Section D Structural Biology
- Published
- 2018-09-01
- Volume
- 74
- Issue
- 9
- Pages
- 814-840
- ISSN
- 2059-7983 (ISSN-L)
- OpenAlex
- W2791670278
- Cited by
- 755
- References
- 6
- FWCI
- 30.17
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Afonine, P. V., Klaholz, B. P., Moriarty, N. W., Poon, B. K., Sobolev, O. V., Terwilliger, T. C., Adams, P. D., & Urzhumtsev, A. (2018). New tools for the analysis and validation of cryo-EM maps and atomic models. Acta Crystallographica Section D Structural Biology. https://doi.org/10.1107/s2059798318009324
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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