Cited by 25Fungal and yeast genetics researchAntifungal resistance and susceptibilityBacterial Genetics and Biotechnology

The putative transcription factor CaRtg3 is involved in tolerance to cations and antifungal drugs as well as serum-induced filamentation in Candida albicans

Yan, Hongbo, Zhao, Yunying, Jiang, Linghuo·2014·FEMS Yeast Research

Abstract

The activated retrograde (RTG) pathway controls transcription of target genes through a heterodimer of transcription factors, Rtg1 and Rtg3, in Saccharomyces cerevisiae. Here, we have identified the sole homologous gene CaRTG3 that encodes a protein of 520 amino acids with characteristics of the basic helix-loop-helix/leucine zipper (bHLH/Zip) family in Candida albicans. Deletion of CaRTG3 results in C. albicans cells being sensitive to high concentrations of calcium and lithium cations as well as sodium dodecyl sulfate and activates the calcium/calcineurin signaling pathway in C. albicans cells. CaRTG3 is also involved in the tolerance of C. albicans cells to the antifungal drugs azoles and terbinafine, but not to the antifungal drugs casponfungin and amphotericin B as well as the cell-wall-damaging reagents Calcoflour White and Congo red. In contrast to ScRtg3, CaRtg3 is not involved in the osmolar response and is constitutively localized in the nucleus. However, deletion of CaRTG3 results in a delay in serum-induced filamentation of C. albicans cells. Therefore, CaRtg3 plays a role in tolerance to cations and antifungal drugs as well as serum-induced filamentation in C. albicans.

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Registry

Crossref · 10.1111/1567-1364.12148

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  • Earlier work in respiratory competent C. albicans indicated that the Rtg pathway is involved in processes related to infectivity, filamentous growth and niche adaptation, crucial for the commensal/opportunistic pathogen lifestyle of this yeast species.

    “Earlier work performed in respiratory competent C. albicans indicated that the Rtg pathway is involved in the processes related to infectivity, filamentous growth and niche adaptation, crucial for the commensal/opportunistic pathogen lifestyle of this yeast species (Pérez et al. 2013; Yan et al. 2014).”

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Authors
Yan, H., Zhao, Y., & Jiang, L.
Affiliations
Jiangnan University (CN)
Corresponding author
Jiang, Linghuo
Venue
FEMS Yeast Research
Published
2014-03-26
Volume
14
Issue
4
Pages
614-623
ISSN
1567-1356 (ISSN-L)
OpenAlex
W2152386601
Cited by
25
References
54
Citations by year
2026: 2 · 2025: 1 · 2023: 1 · 2022: 1 · 2021: 3 · 2020: 5 · 2019: 3 · 2018: 5 · 2017: 1 · 2015: 2 · 2014: 1
FWCI
0.55
Type
journal article
Language
en
Open access
closed
Locations
  • FEMS Yeast Research (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
retrograde pathway, Candida albicans, calcium tolerance, lithium tolerance, sodium dodecyl sulfate, antifungal drug tolerance, azoles, terbinafine, bHLH-Zip transcription factors, osmotic response
MeSH terms
Terbinafine, Antifungal Agents, Azoles, Calcium, Candida albicans, Cations, Drug Tolerance, Lithium, Naphthalenes, Saccharomyces cerevisiae, Sodium Dodecyl Sulfate, Transcription Factors, Gene Expression Regulation, Fungal, Gene Deletion, Hyphae, Serum

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