Does Bt maize expressing Cry1Ac protein have adverse effects on the parasitoid Macrocentrus cingulum (Hymenoptera: Braconidae)?
Abstract
The potential effects of insect-resistant, genetically engineered (GE) crops on non-target organisms, especially on predators and parasitoids, must be evaluated before their commercial cultivation. The effects of GE maize that produces Cry1Ac toxin on the parasitoid Macrocentrus cingulum were assessed by direct bioassay and indirect bioassay. In the indirect bioassay, parasitism rate, cocoon weight and the number of M. cingulum progeny produced per host were significantly reduced when M. cingulum-parasitized Cry1Ac-susceptible Ostrinia furnacalis were fed a diet containing purified Cry1Ac; however, life-table parameters of M. cingulum were not adversely affected when the same assay was performed with Cry1Ac-resistant O. furnacalis. These results indicated that the detrimental effects detected with a Cry1Ac-susceptible host were mediated by poor host quality. In a direct bioassay, no difference in life-table parameters were detected when M. cingulum adults were directly fed a 20% honey solution with or without Cry1Ac; however, survival and longevity were significantly reduced when M. cingulum adults were fed a honey solution containing potassium arsenate, which was used as a positive control. The stability and bioactivity of Cry1Ac toxin in the food sources and Cry1Ac toxin uptake by the host insect and parasitoid were confirmed by enzyme-linked immunosorbent assay and sensitive-insect bioassays. Our results demonstrate that M. cingulum is not sensitive to Cry1Ac toxin at concentrations exceeding those encountered in Bacillus thuringiensis maize fields. This study also demonstrates the power of using resistant hosts when assessing the risk of genetically modified plants on non-target organisms and will be useful for assessing other non-target impacts.
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The importance of prey/host-quality effects has been demonstrated experimentally for parasitoids Diadegma insulare and Macrocentrus cingulum and predators C. carnea and C. maculata.
“The importance of such prey/host-quality effects has been demonstrated experimentally for the parasitoids Diadegma insulare (Hymenoptera: Ichneumonidae) (Chen et al., 2008) and Macrocentrus cingulum (Hymenoptera: Braconidae) (Wang et al., 2017) and for the predators C. carnea (Lawo et al., 2010) and C. maculata (Li et al., 2011).”
From Genetically engineered crops help support conservation biological control (2019) · cites this paper as Wang et al., 2017
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Genetically engineered crops help support conservation biological control
Romeis, J., Naranjo, S. E., Meissle, M., & Shelton, A. M. (2019) · Biological Control · doi:10.1016/j.biocontrol.2018.10.001 · Cited by 231 · see citing works
- Authors
- Wang, Z., Li, Y., He, K., Bai, S., Zhang, T., Cai, W., & Wang, Z.
- Affiliations
- Institute of Plant Protection (CN)
- State Key Laboratory for Biology of Plant Diseases and Insect Pests, MOA – CABI Joint Laboratory for Bio‐safety Institute of Plant Protection, Chinese Academy of Agricultural Science Beijing China
- Department of Entomology China Agriculture University Beijing China
- Venue
- Insect Science
- Publisher
- Wiley
- Published
- 2016-06-23
- Volume
- 24
- Issue
- 4
- Pages
- 599-612
- ISSN
- 1005-295X (ISSN-L)
- 1672-9609
- 1744-7917
- OpenAlex
- W2343346263
- Cited by
- 14
- References
- 46
- Citations by year
- 2024: 1 · 2023: 2 · 2021: 2 · 2020: 3 · 2018: 6
- FWCI
- 1.04
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Insect Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Genetically Modified Organisms Breeding Major Projects (2014ZX08011-003)
- Special Fund for Agro-scientific Research in the Public Interest (201303026)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect-Plant Interactions and Control
- Keywords
- Bt maize, Cry1Ac toxin, Macrocentrus cingulum, parasitoids, non-target effects, genetically modified crops, Ostrinia furnacalis, parasite prevalence, host quality, life table parameters, potassium arsenate, non-target risk assessment
- MeSH terms
- Animals, Arsenates, Bacillus thuringiensis, Bacterial Proteins, Zea mays, Endotoxins, Hemolysin Proteins, Host-Parasite Interactions, Larva, Moths, Pest Control, Biological, Wasps, Plants, Genetically Modified
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