Extrapolating non-target risk of Bt crops from laboratory to field
Open accessAbstract
The tiered approach to assessing ecological risk of insect-resistant transgenic crops assumes that lower tier laboratory studies, which expose surrogate non-target organisms to high doses of insecticidal proteins, can detect harmful effects that might be manifested in the field. To test this assumption, we performed meta-analyses comparing results for non-target invertebrates exposed to Bacillus thuringiensis ( Bt ) Cry proteins in laboratory studies with results derived from independent field studies examining effects on the abundance of non-target invertebrates. For Lepidopteran-active Cry proteins, laboratory studies correctly predicted the reduced field abundance of non-target Lepidoptera. However, laboratory studies incorporating tri-trophic interactions of Bt plants, herbivores and parasitoids were better correlated with the decreased field abundance of parasitoids than were direct-exposure assays. For predators, laboratory tri-trophic studies predicted reduced abundances that were not realized in field studies and thus overestimated ecological risk. Exposure to Coleopteran-active Cry proteins did not significantly reduce the laboratory survival or field abundance of any functional group examined. Our findings support the assumption that laboratory studies of transgenic insecticidal crops show effects that are either consistent with, or more conservative than, those found in field studies, with the important caveat that laboratory studies should explore all ecologically relevant routes of exposure.
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Several meta-analyses of laboratory data also concluded that Cry1/Cry2 proteins are specific to Lepidoptera and Cry3 to Chrysomelidae.
“This also has been concluded from several meta-analyses of laboratory data [11, 12, 14].”
From Does the growing of Bt maize change abundance or ecological function of non-target animals compared to the growing of non-GM maize? A systematic review (2022) · cites this paper as [12]
Previous meta-analyses selected data on the finest available taxonomic level.
“Previous meta-analyses [10, 12–14] selected data on the finest available taxonomic level.”
From Does the growing of Bt maize change abundance or ecological function of non-target animals compared to the growing of non-GM maize? A systematic review (2022) · cites this paper as [12]
Early tier laboratory studies can conservatively predict non-target effects expected in the field.
“Further, it has been suggested that such early tier laboratory studies can conservatively predict non-target effects expected in the field (Duan et al., 2010).”
From Genetically engineered crops help support conservation biological control (2019) · cites this paper as Duan et al., 2010
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Indirect exposure to Bt maize through pig faeces causes behavioural changes in dung beetles
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- Authors
- Duan, J. J., Lundgren, J. G., Naranjo, S., & Marvier, M.
- Affiliations
- Agricultural Research Service (US)
- Santa Clara University (US)
- Corresponding author
- Marvier, Michelle
- Venue
- Biology Letters
- Publisher
- The Royal Society
- Published
- 2009-09-09
- Volume
- 6
- Issue
- 1
- Pages
- 74-77
- ISSN
- 1744-9561 (ISSN-L)
- 1744-957X
- PMC
- PMC2817261
- OpenAlex
- W2126159729
- Cited by
- 127
- References
- 9
- Citations by year
- 2026: 1 · 2025: 4 · 2023: 2 · 2022: 9 · 2021: 2 · 2020: 5 · 2019: 6 · 2018: 6 · 2017: 5 · 2016: 6 · 2015: 13 · 2014: 12 · 2013: 15 · 2012: 14
- FWCI
- 16.16
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Biology Letters (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
- Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect-Plant Interactions and Control
- Keywords
- Bt crops, non-target invertebrates, environmental risk assessment, laboratory-to-field extrapolation, tritrophic interactions, parasitoids, predators, Lepidoptera, Coleoptera, insecticidal proteins
- MeSH terms
- Bacillus thuringiensis Toxins, Animals, Bacterial Proteins, Endotoxins, Hemolysin Proteins, Insecticides, Invertebrates, Pest Control, Biological, Species Specificity, Crops, Agricultural, Risk Assessment, Plants, Genetically Modified
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