Cited by 90Aquaculture Nutrition and GrowthAquaculture disease management and microbiotaCrustacean biology and ecology

Growth and immune response of giant freshwater prawn Macrobrachium rosenbergii (De Man) postlarvae fed diets containing Chlorella vulgaris (Beijerinck)

Maliwat, Gian Carlo, Velasquez, Stephanie, Robil, Jan Lorie, Chan, Merab, Traifalgar, Rex Ferdinand, Tayamen, Melchor, Ragaza, Janice A·2017·Aquaculture Research

Abstract

A 50-day growth trial was conducted to evaluate the efficacy of Chlorella vulgaris (Beijerinck) as an ingredient in the diets of giant freshwater prawn Macrobrachium rosenbergii (De Man) postlarvae (PL30). Immune response (total haemocyte count and prophenoloxidase activity) was also assessed by subjecting postlarvae to a challenge test against Aeromonas hydrophila (Chester) for 14 days. Iso-nitrogenous and iso-lipidic test diets were prepared using a fishmeal-based-positive control diet (D0) and four basal diets with inclusion levels of 2% (D2), 4% (D4), 6% (D6) and 8% (D8) C. vulgaris. Postlarvae of M. rosenbergii were randomly stocked (mean initial body weight of 0.19 ± 0.02 g) in 30-L tanks in three replicates per dietary treatment for evaluation of growth performance. Another set of postlarvae (mean initial body weight of 1.25 ± 0.02 g) was randomly distributed in 95-L tanks in three replicates per dietary treatment for the assessment of immune response. Results showed that specific growth rate was significantly higher (P < 0.05) in postlarvae fed D4 and D6. Variations in values for carcass protein, lipid, moisture and ash were also evident. Postlarvae fed diets with Chlorella showed increased prophenol oxidase activity and total haemocyte counts. Moreover, survival rate after challenge with A. hydrophila was significantly increased (P < 0.05). Inclusion of C. vulgaris in diets enhanced immune response and resistance of M. rosenbergii postlarvae against A. hydrophila infection.

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Crossref · 10.1111/are.13004

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  • When 6-8% of fish meal was replaced with Chlorella vulgaris, postlarvae of Macrobrachium rosenbergii displayed improved immune response and survivability against Aeromonas hydrophila infection.

    “When 6-8% of fish meal was replaced with Chlorella vulgaris, postlarvae of Macrobrachium rosenbergii displayed improved immune response (prophenol oxidase activity and total haemocyte count), and survivability against Aeromonas hydrophila infection (Maliwat et al., 2017).”

    From Potential of microalgae as a sustainable feed ingredient for aquaculture (2021) · cites this paper as Maliwat et al., 2017

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Authors
Maliwat, G. C., Velasquez, S., Robil, J. L., Chan, M., Traifalgar, R. F., Tayamen, M., & Ragaza, J. A.
Affiliations
  • Ateneo de Manila University (PH)
  • University of the Philippines Visayas (PH)
  • Bureau of Fisheries and Aquatic Resources National Freshwater Fisheries Technology Center Muñoz Science City; Nueva Ecija Philippines
Corresponding author
Ragaza, Janice A
Venue
Aquaculture Research
Published
2016-02-04
Volume
48
Issue
4
Pages
1666-1676
ISSN
1355-557X (ISSN-L)
OpenAlex
W2506794043
Cited by
90
References
57
Citations by year
2026: 9 · 2025: 15 · 2024: 6 · 2023: 17 · 2022: 17 · 2021: 6 · 2020: 8 · 2019: 5 · 2018: 3 · 2017: 4
FWCI
5.72
Type
journal article
Language
en
Open access
closed
Locations
Aquaculture Research (publishedVersion)
Funders
  • University Research Council Grant
  • Department of Science and Technology Student Research Support Fund (SRSF)
  • Accelerated Science and Technology Human Resource Development Program (ASTHRDP)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
  • Life Sciences › Immunology and Microbiology › Immunology › Aquaculture disease management and microbiota
  • Physical Sciences › Environmental Science › Ecology › Crustacean biology and ecology
Keywords
Macrobrachium rosenbergii, Chlorella vulgaris, postlarvae, alternative feed ingredients, fish meal replacement, growth performance, immune response, hemocyte count, Aeromonas hydrophila challenge, disease resistance, carcass composition

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