Cited by 150Adipose Tissue and MetabolismMuscle Physiology and DisordersMetabolism, Diabetes, and Cancer

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Characterization of the metabolic effects of irisin on skeletal muscle in vitro

Vaughan, R. A., Gannon, N. P., Barberena, M. A., Garcia‐Smith, R., Bisoffi, M., Mermier, C. M., Conn, C. A., Trujillo, K. A.·2014·Diabetes Obesity and Metabolism

DOI 10.1111/dom.12268

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Abstract

AIMS: This work explored the effects of irisin on metabolism, gene expression and mitochondrial content in cultured myocytes. METHODS: C2C12 myocytes were treated with various concentrations of irisin for various durations. Glycolysis and oxidative metabolism were quantified by measurement of extracellular acidification and oxygen consumption, respectively. Metabolic gene expression was measured by quantitative real-time polymerase chain reaction (qRT-PCR) and mitochondrial content was assessed by flow cytometry and confocal microscopy. RESULTS: Cells treated with irisin exhibited significantly increased oxidative metabolism. Irisin treatment also significantly increased mitochondrial uncoupling at various doses and durations. Lastly, treatment with irisin also significantly elevated metabolic gene expression including peroxisome proliferator-activated receptor γ coactivator-1 alpha (PGC-1α), nuclear respiratory factor 1 (NRF1), mitochondrial transcription factor A (TFAM), irisin, glucose transporter 4 (GLUT4) and mitochondrial uncoupling protein 3 (UCP3) leading to increased mitochondrial biogenesis. CONCLUSIONS: Our observations are the first to document increased metabolism in myocytes through irisin-mediated induction of mitochondrial biogenesis and uncoupling with corresponding gene expression. These observations support the need for further investigation into the therapeutic and pharmacological effects of irisin, as well as development of irisin-based therapy.

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Mechanisms controlling mitochondrial biogenesis and respiration through the thermogenic coactivator PGC-1
Wu, Z., Puigserver, P., Andersson, U., Zhang, C., Adelmant, G., Mootha, V., Troy, A., Cinti, S., Lowell, B., Scarpulla, R. C., et al. (1999) · Cell · doi:10.1016/s0092-8674(00)80611-x · Cited by 4,139
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Activation of the human mitochondrial transcriptionfactor A gene by nuclear respiratory factors: a potential regulatory linkbetween nuclear and mitochondrial gene expression in organellebiogenesis.
Virbasius, J. V., & Scarpulla, R. C. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.4.1309 · Cited by 819
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NRF-1: a trans-activator of nuclear-encoded respiratory genes in animal cells.
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Li, J., Xie, S.-S., Guo, L., Jiang, J., & Chen, H. (2020) · PubMed Central · Cited by 15
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Authors: Vaughan, R. A., Gannon, N. P., Barberena, M. A., Garcia‐Smith, R., Bisoffi, M., Mermier, C. M., Conn, C. A., & Trujillo, K. A.
Affiliations: University of New Mexico (US)
Corresponding author: Vaughan, R. A.
ORCID: Vaughan, R. A.
Gannon, N. P.
Bisoffi, M.
Mermier, C. M.
Trujillo, K. A.
Venue: Diabetes Obesity and Metabolism
Publisher: Wiley
Published: 2014-02-18
Volume: 16
Issue: 8
Pages: 711-718
ISSN: 1462-8902 (ISSN-L)
1463-1326
DOI: 10.1111/dom.12268
OpenAlex: W2000249408
Cited by: 150
References: 19
Citations by year: 2026: 7 · 2025: 11 · 2024: 11 · 2023: 10 · 2022: 15 · 2021: 15 · 2020: 14 · 2019: 15 · 2018: 5 · 2017: 14 · 2016: 9 · 2015: 15 · 2014: 9
FWCI: 10.41
Type: journal article
Language: en
Open access: closed
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations: Diabetes Obesity and Metabolism (publishedVersion)
PubMed (publishedVersion)
Funders: 2013 University of New Mexico- New Mexico Research Grant
Topics: Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
Keywords: irisin, skeletal muscle, C2C12 myocytes, mitochondrial biogenesis, mitochondrial uncoupling, oxidative metabolism, glycolysis, PGC-1α, NRF1, TFAM, GLUT4, UCP3, metabolic gene expression, mitochondrial content
MeSH terms: Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha, Animals, Cell Line, Cell Survival, DNA-Binding Proteins, Fibronectins, Gene Expression Regulation, Glycolysis, High Mobility Group Proteins, Humans, Kinetics, Mitochondria, Muscle, Muscle Proteins, Oxidative Phosphorylation, Recombinant Proteins, Transcription Factors, Muscle Fibers, Skeletal, Cell Proliferation, Nuclear Respiratory Factor 1, Mice, Mitochondrial Turnover

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Characterization of the metabolic effects of irisin on skeletal muscle in vitro

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