Perineuronal nets affect parvalbumin expression in GABA ergic neurons of the mouse hippocampus
Abstract
Recent studies have suggested that the perineuronal net ( PNN ), a specialised extracellular matrix structure, and parvalbumin (PV), an EF‐hand calcium‐binding protein, are involved in the regulation of plasticity of neural circuits. Here, we aimed to quantitatively estimate the relationship between the two plasticity regulators, PV and PNNs, in the hippocampus of young adult mice. Dual fluorescence staining for PV and Wisteria floribunda agglutinin (a broad PNN marker) showed that a substantial population of PV‐expressing (PV + ) GABAergic neurons lacked PNNs. Optical disector analysis demonstrated that there were fewer PNN + neurons than PV + neurons. The ratio of PNN expression in PV + neurons was generally lower in the dendritic layers than in the principal cell layers, whereas the ratio of PV expression in PNN + neurons was effectively 100%. The mean PV fluorescence was significantly higher in PNN + /PV + neurons than in PNN − /PV + neurons. Cumulative frequencies for single‐cell PV fluorescence indicated that intensely stained PV + neurons tend to be enwrapped by PNNs, whereas weakly stained PV + neurons are likely to lack PNNs. We digested the PNNs by a unilateral injection of chondroitinase ABC (chABC) into the dorsal CA1 region. Although the densities of PV + neurons remained unchanged, the PV fluorescence declined 7 days after chABC injection. Quantitative real‐time polymerase chain reaction analysis demonstrated a reduction in PV mRNA expression following chABC injection. These findings indicate that the presence or absence of PNNs affects the relative PV expression in GABAergic neurons in the hippocampus.
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Experimental disruptions of perineuronal nets result in lower parvalbumin mRNA expression without a change in the number of detectable parvalbumin cells.
“Any of these mechanisms could lead to lower PV levels as experimental disruptions of PNNs results in lower PV mRNA expression (Yamada et al, 2015), without a change in the number of detectable PV cells (Cabungcal et al, 2013a), findings that match the current report.”
From Reduced Labeling of Parvalbumin Neurons and Perineuronal Nets in the Dorsolateral Prefrontal Cortex of Subjects with Schizophrenia (2016) · cites this paper as Yamada et al, 2015
In the hippocampus, PNNs primarily surround inhibitory interneurons, many of which are fast-spiking PV+ interneurons.
“In the hippocampus, PNNs primarily surround inhibitory interneurons, many of which are fast-spiking PV+ interneurons (Lensjø et al., 2017b; Yamada et al., 2015).”
From Adult neurogenesis, glia, and the extracellular matrix (2019) · cites this paper as Yamada et al., 2015
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Self-citation
- Authors
- Yamada, J., Ohgomori, T., & Jinno, S.
- Affiliations
- Kyushu University (JP)
- Corresponding author
- Jinno, S.
- Venue
- European Journal of Neuroscience
- Publisher
- Wiley
- Published
- 2014-11-20
- Volume
- 41
- Issue
- 3
- Pages
- 368-378
- ISSN
- 0953-816X (ISSN-L)
- 1460-9568
- OpenAlex
- W2054505706
- Semantic Scholar
- 5f1fa17eece49d0d97517b4ef7115dfb27a97e2f
- Cited by
- 99
- References
- 12
- Citations by year
- 2026: 6 · 2025: 10 · 2024: 8 · 2023: 6 · 2022: 7 · 2021: 10 · 2020: 8 · 2019: 6 · 2018: 14 · 2017: 14 · 2016: 8 · 2015: 2
- FWCI
- 3.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- European Journal of Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Keywords
- perineuronal nets, parvalbumin, GABAergic neurons, hippocampus, extracellular matrix, chondroitinase ABC, CA1, neuroplasticity, Wisteria floribunda agglutinin
- MeSH terms
- Animals, Extracellular Matrix, Hippocampus, Male, Mice, Inbred C57BL, Parvalbumins, Photomicrography, RNA, Messenger, Chondroitin ABC Lyase, Receptors, N-Acetylglucosamine, Plant Lectins, GABAergic Neurons, Proteolysis, Real-Time Polymerase Chain Reaction, Optical Imaging
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