The LIM homeobox gene, L3/Lhx8, is necessary for proper development of basal forebrain cholinergic neurons
Abstract
Basal forebrain cholinergic neurons (BFCNs) are involved in cognitive functions such as learning and memory, and are affected in several neurodegenerative diseases (e.g. Alzheimer's disease). Despite their importance, the molecular mechanisms of their development are not fully elucidated. A recent report demonstrated that some BFCNs in adult rat are positive for L3/Lhx8, a LIM homeobox transcription factor. To examine the function of L3/Lhx8 in the development of BFCNs, we generated L3/Lhx8 gene-disrupted mice. In these mice, cells expressing cholinergic neuron markers, such as choline acetyltransferase, vesicular acetylcholine transporter and p75 low-affinity NGF receptor, were markedly reduced in the basal forebrain, whereas other cholinergic neurons including brain stem and spinal motor neurons expressed the markers. Neurotransmitter phenotypes other than cholinergic in the basal forebrain appeared intact. From these results, we suggested that L3/Lhx8 has a pivotal and specific role in the development and/or maintenance of BFCNs.
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Gbx1 and Lhx8 are regulators of cholinergic neuron development.
“Gbx1 and Lhx8 that are regulators of cholinergic neuron development [52,53]”
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- Authors
- Mori, T., Yuxing, Z., Takaki, H., Takeuchi, M., Iseki, K., Hagino, S., Kitanaka, J., Takemura, M., Misawa, H., Ikawa, M., Okabe, M., & Wanaka, A.
- Affiliations
- Fukushima Medical University (JP)
- Institute of Biomedical Science (GB)
- Hyogo Medical University (JP)
- The University of Osaka (JP)
- Nara Medical University (JP)
- Corresponding author
- Wanaka, Akio
- Venue
- European Journal of Neuroscience
- Publisher
- Wiley
- Published
- 2004-06
- Volume
- 19
- Issue
- 12
- Pages
- 3129-3141
- ISSN
- 0953-816X (ISSN-L)
- 1460-9568
- OpenAlex
- W2120869510
- Cited by
- 99
- References
- 2
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 1 · 2023: 2 · 2022: 4 · 2020: 9 · 2019: 5 · 2018: 3 · 2017: 3 · 2016: 1 · 2015: 5 · 2014: 5 · 2013: 6 · 2012: 3
- FWCI
- 1.78
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- European Journal of Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- LIM homeobox transcription factor, basal forebrain cholinergic neurons, choline acetyltransferase, vesicular acetylcholine transporter, p75 NGF receptor, Alzheimer's disease, neurotransmitter phenotype
- MeSH terms
- Animals, Cholinergic Fibers, Genes, Homeobox, Immunohistochemistry, Nerve Tissue Proteins, Phenotype, Transcription Factors, Gene Expression, Prosencephalon, In Situ Hybridization, Mice, Knockout, Homeodomain Proteins, Reverse Transcriptase Polymerase Chain Reaction, Mice, LIM-Homeodomain Proteins
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