Human mesenchymal stem cells promote growth of osteosarcoma: involvement of interleukin-6 in the interaction between human mesenchymal stem cells and Saos-2
Open accessAbstract
Our previous study showed that exogenous human mesenchymal stem cells (hMSCs) targeted established osteosarcoma and promoted its growth and pulmonary metastasis in vivo. As a follow‐up, the present study aimed to investigate how hMSCs would interact with Saos‐2 through autocrine/paracrine communication. The results showed that co‐injection of hMSCs with Saos‐2 into the proximal tibia of nude mice could promote tumor growth and progression. In vitro, the proliferation of Saos‐2 and hMSCs was promoted by each other’s conditioned medium, in which interleukin‐6 (IL‐6) played an important role. Osteogenic differentiation of hMSCs could be inhibited by conditioned medium of Saos‐2, in which IL‐6 was also involved. Furthermore, decreased IL‐6 secretion by hMSCs during its osteogenesis and increased IL‐6 secretion in response to conditioned medium of Saos‐2 were observed. Based on these data, we suggest that there was a positive feedback loop of IL‐6 in the interaction between hMSCs and Saos‐2. (Cancer Sci 2010; 101: 2554–2560)
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Elevated IL-6 secretion by MSC has been detected after interaction with malignant cells in several epithelial, hematopoietic and mesenchymal cancers.
“Secretion of elevated levels of IL-6 by MSC has been detected upon interaction with malignant cells in several epithelial, hematopoietic and mesenchymal cancers (Table 2) [43, 69, 76, 77, 82, 115–119].”
From Mesenchymal stem cell secretome and regenerative therapy after cancer (2013) · cites this paper as [118]
The section on paracrine interaction reports that IL-6 secretion by human MSCs increased when they were exposed to conditioned medium from Saos-2 osteosarcoma cells. This supports the claim for a mesenchymal cancer, but the paper does not establish elevated MSC IL-6 secretion after interaction with malignant cells across several epithelial and hematopoietic cancers. (LLM, conf=0.85)
Experimental — accuracy not yet measuredInterleukin-6 plays a pivotal role in the bidirectional proliferation of human mesenchymal stromal cells with Saos-2 both in vitro and in nude mice models.
“Interleukin-6 (IL-6) seems to play a pivotal role in the bidirectional proliferation of human MSCs (hMSCs) with Saos-2 both in vitro and nude mice models.”
From Mesenchymal stromal cells for bone sarcoma treatment: Roadmap to clinical practice (2019) · cites this paper as [98]
The ‘Interaction between hMSCs and Saos-2 through paracrine’ section reports that IL-6 was essential to the reciprocal proliferation effects of their conditioned media in vitro. The Results report greater tumor growth after hMSCs and Saos-2 were co-injected into nude mice, but the mouse experiment did not establish IL-6’s role or bidirectional proliferation there. Page numbers were not available in our copy. (LLM, conf=0.87)
Experimental — accuracy not yet measuredInterleukin-6 produced from the conditioned medium of Saos-2 could inhibit osteogenic differentiation of human mesenchymal stromal cells.
“Interestingly, IL-6 produced from the conditioned medium of Saos-2 could inhibit osteogenic differentiation of hMSCs [98].”
From Mesenchymal stromal cells for bone sarcoma treatment: Roadmap to clinical practice (2019) · cites this paper as [98]
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- Authors
- Bian, Z., Fan, Q., Li, G., Xu, W., & Tang, T.
- Affiliations
- Shanghai Jiao Tong University (CN)
- Chinese University of Hong Kong (HK)
- ORCID
- Li, Gang
- Venue
- Cancer Science
- Publisher
- Wiley
- Published
- 2010-09-28
- Volume
- 101
- Issue
- 12
- Pages
- 2554-2560
- ISSN
- 1347-9032 (ISSN-L)
- 1349-7006
- PMC
- PMC11159660
- OpenAlex
- W2046916147
- Cited by
- 83
- References
- 36
- Citations by year
- 2025: 1 · 2024: 3 · 2023: 6 · 2022: 1 · 2021: 7 · 2020: 3 · 2019: 4 · 2018: 7 · 2017: 7 · 2016: 14 · 2015: 9 · 2014: 5 · 2013: 7 · 2012: 7
- FWCI
- 2.28
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Cancer Science (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Shanghai Science and Technology Development Foundation
- Shanghai Municipal Education Commission (J50206)
- Shanghai Key Laboratory of Orthopaedic Implants
- Program for New Century Excellent Talents in University
- Topics
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Sarcoma Diagnosis and Treatment
- Keywords
- human mesenchymal stem cells, osteosarcoma, Saos-2 cells, interleukin-6, tumor growth promotion, paracrine signaling, autocrine signaling, osteogenic differentiation, tumor microenvironment, lung metastasis
- MeSH terms
- Animals, Bone Neoplasms, Cell Communication, Cell Differentiation, Enzyme-Linked Immunosorbent Assay, Humans, Mice, Nude, Osteosarcoma, Interleukin-6, Xenograft Model Antitumor Assays, RNA, Small Interfering, Cell Line, Tumor, Mice, Mesenchymal Stem Cells
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