Ancestral Matrilineages and Mitochondrial DNA Diversity of the Lidia Cattle Breed
Abstract
To clarify the genetic ancestry and the mitochondrial DNA (mtDNA) diversity of the Lidia cattle breed, a 521-bp D-loop fragment was sequenced in 527 animals belonging to 70 herds distributed across 29 lineages. The mtDNA diversity recorded was similar to that seen for Middle Eastern breeds and greater than that recorded for the majority of European breeds. Haplotype T3 was the most common (81%), followed by the African T1 haplotype (17%); very low frequencies were recorded for haplotypes T and T2. The results agree with there being two major ancestral lines for the Lidia breed, European and African, similar to that seen for other Mediterranean breeds. A wide range of variation in haplotype frequencies was seen between the examined lineages. Haplotype T3 was present in all those analysed; in five it was the only one present, and in only one lineage (Miura) was its frequency lower than that of T1. T1*, a haplotype reported in Criollo breeds and to date in only a single European breed (the Retinta breed from Spain), was found in a single animal belonging to the Concha y Sierra lineage. Network analysis of the Lidia breed revealed the presence of two major haplotypes: T3 and T1. The Lidia breed appears to be more closely related to prehistoric Iberian and Italian than to British aurochs.
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In the 15th century, southern European herds carrying African indicine ancestry were introduced to the Americas.
“Subsequently, in the 15th century, southern European herds carrying African indicine ancestry were introduced to the Americas [52,53].”
From The Genomic Landscape of Cattle: Domestication, Dispersal, and Adaptive Evolution (2026) · cites this paper as [52]
African cattle (mtDNA T1, mixed with zebu lineage) were once introduced to the European Mediterranean region.
“As previously mentioned, African cattle (mtDNA T1, mixed with zebu lineage) were once introduced to the European Mediterranean region [26,52,53].”
From The Genomic Landscape of Cattle: Domestication, Dispersal, and Adaptive Evolution (2026) · cites this paper as [52]
Cites
Molecular cloning: A laboratory manual
Deininger, P. L. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90595-z · Cited by 84,684 · see citing works
Arlequin (version 3.0): An integrated software package for population genetics data analysis
Excoffier, L., Laval, G., & Schneider, S. (2005) · Evolutionary Bioinformatics · doi:10.1177/117693430500100003 · Cited by 14,194 · see citing works
Median-joining networks for inferring intraspecific phylogenies
Bandelt, H. J., Forster, P., & Rohl, A. (1999) · Molecular Biology and Evolution · doi:10.1093/oxfordjournals.molbev.a026036 · Cited by 11,742 · see citing works
MEGA2: molecular evolutionary genetics analysis software
Kumar, S., Tamura, K., Jakobsen, I. B., & Nei, M. (2001) · Bioinformatics · doi:10.1093/bioinformatics/17.12.1244 · Cited by 6,046 · see citing works
Panova, M., Hollander, J., & Johannesson, K. (2006) · Molecular Ecology · doi:10.1111/j.1365-294x.2006.03067.x · Cited by 2,469 · see citing works
Anderson, S., de Bruijn, M. H. L., Coulson, A. R., Eperon, I. C., Sanger, F., & Young, I. G. (1982) · Journal of Molecular Biology · doi:10.1016/0022-2836(82)90137-1 · Cited by 1,472 · see citing works
Evidence for Two Independent Domestications of Cattle
Loftus, R. T., MacHugh, D. E., Bradley, D. G., Sharp, P. M., & Cunningham, P. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.7.2757 · Cited by 812 · see citing works
Genetic Evidence for Near-Eastern Origins of European Cattle
Troy, C. S., MacHugh, D. E., Bailey, J. F., Magee, D. A., Loftus, R. T., Cunningham, P., Chamberlain, A. T., Sykes, B. C., & Bradley, D. G. (2001) · Nature · doi:10.1038/35074088 · Cited by 627 · see citing works
Mitochondrial diversity and the origins of African and European cattle
Bradley, D. G., MacHugh, D. E., Cunningham, P., & Loftus, R. T. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.10.5131 · Cited by 584 · see citing works
The Origin of European Cattle: Evidence from Modern and Ancient DNA
Beja-Pereira, A., Caramelli, D., Lalueza-Fox, C., Vernesi, C., Ferrand, N., Casoli, A., Goyache, F., Royo, L. J., Conti, S., Lari, M., et al. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0509210103 · Cited by 342 · see citing works
Neolithic transition in Europe: the radiocarbon record revisited
Gkiasta, M., Russell, T., Shennan, S. J., & Steele, J. (2003) · Antiquity · doi:10.1017/s0003598x00061330 · Cited by 210 · see citing works
Mannen, H., Kohno, M., Nagata, Y., Tsuji, S., Bradley, D. G., Yeo, J. S., Nyamsamba, D., Zagdsuren, Y., Yokohama, M., Nomura, K., et al. (2004) · Molecular Phylogenetics and Evolution · doi:10.1016/j.ympev.2004.01.010 · Cited by 187 · see citing works
Genes of domestic mammals augmented by backcrossing with wild ancestors
Vilà, C., Seddon, J. M., & Ellegren, H. (2005) · Trends in Genetics · doi:10.1016/j.tig.2005.02.004 · Cited by 153 · see citing works
Anderung, C., Bouwman, A., Persson, P., Carretero, J. M., Martínez, A. I. O., Elburg, R., Smith, C. I., Arsuaga, J. L., Ellegren, H., & Götherström, A. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0503396102 · Cited by 134 · see citing works
Mitochondrial sequence variation suggests an African influence in Portuguese cattle
Cymbron, T., Loftus, R. T., Malheiro, I., & Bradley, D. G. (1999) · Proceedings of the Royal Society B Biological Sciences · doi:10.1098/rspb.1999.0678 · Cited by 114 · see citing works
The Spread of Early Farming in Europe
Bogucki, P. (1996) · American Scientist · Cited by 93 · see citing works
Ancient DNA analysis of 101 cattle remains: limits and prospects
Edwards, C. J., MacHugh, D. E., Dobney, K. M., Martin, L. A., Russell, N., Horwitz, L. K., McIntosh, S. K., MacDonald, K. C., Helmer, D., Tresset, A., et al. (2004) · Journal of Archaeological Science · doi:10.1016/j.jas.2003.11.001 · Cited by 85 · see citing works
Miretti, M. M., Dunner, S., Naves, M., Contel, E. P., & Ferro, J. A. (2004) · Journal of Heredity · doi:10.1093/jhered/esh070 · Cited by 56 · see citing works
Self-citationGenetic variation within the Lidia bovine breed
Cañón, J., Tupac-Yupanqui, I., García‐Atance, M. A., Cortés, O., García, D., Fernández, J., & Dunner, S. (2008) · Animal Genetics · doi:10.1111/j.1365-2052.2008.01738.x · Cited by 50 · see citing works
Self-citationA numerical description of breed structure
Robertson, A. (1953) · The Journal of Agricultural Science · doi:10.1017/s0021859600057518 · Cited by 47 · see citing works
Miretti, M. M. (2002) · Journal of Heredity · doi:10.1093/jhered/93.5.323 · Cited by 44 · see citing works
African Matrilineages in American Creole Cattle: Evidence of Two Independent Continental Sources
Lirón, J. P., Bravi, C. M., Mirol, P. M., Peral‐García, P., & Giovambattista, G. (2006) · Animal Genetics · doi:10.1111/j.1365-2052.2006.01452.x · Cited by 42 · see citing works
Abundant mtDNA Diversity and Ancestral Admixture in Colombian criollo Cattle (Bos taurus)
Carvajal‐Carmona, L. G., Bermúdez, N. R., Olivera, M., Estrada, L., Ossa, J. E., Bedoya, G., & Ruiz‐Linares, A. (2003) · Genetics · doi:10.1093/genetics/165.3.1457 · Cited by 36 · see citing works
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Achilli, A., Bonfiglio, S., Olivieri, A., Malusà, A., Pala, M., Kashani, B. H., Perego, U. A., Ajmone-Marsan, P., Liotta, L., Semino, O., et al. (2009) · PLoS ONE · doi:10.1371/journal.pone.0005753 · Cited by 212 · see citing works
Ginja, C., Penedo, M. C. T., Melucci, L., Quiroz, J., Martínez López, O. R., Revidatti, M. A., Martínez‐Martínez, A., Delgado, J. V., & Gama, L. T. (2010) · Animal Genetics · doi:10.1111/j.1365-2052.2009.01976.x · Cited by 113 · see citing works
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Bonfiglio, S., Ginja, C., De Gaetano, A., Achilli, A., Olivieri, A., Colli, L., Tesfaye, K., Agha, S. H., Gama, L. T., Cattonaro, F., et al. (2012) · PLoS ONE · doi:10.1371/journal.pone.0038601 · Cited by 106 · see citing works
Ginja, C., Gama, L. T., Cortés, O., Burriel, I. M., Vega-Pla, J. L., Penedo, C., Sponenberg, P., Cañón, J., Sanz, A., do Egito, A. A., et al. (2019) · Scientific Reports · doi:10.1038/s41598-019-47636-0 · Cited by 71 · see citing works
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Ginja, C., Gama, L. T., & Penedo, M. C. T. (2009) · Journal of Heredity · doi:10.1093/jhered/esp104 · Cited by 46 · see citing works
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Cortés, O., Tupac-Yupanqui, I., Dunner, S., Fernández, J., & Cañón, J. (2011) · Journal of Animal Breeding and Genetics · doi:10.1111/j.1439-0388.2011.00951.x · Cited by 25 · see citing works
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Speller, C., Burley, D. V., Woodward, R. P., & Yang, D. Y. (2013) · PLoS ONE · doi:10.1371/journal.pone.0069584 · Cited by 18 · see citing works
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Dorji, J., Jagt, C. V., Chamberlain, A. J., Cocks, B. G., MacLeod, I. M., & Daetwyler, H. D. (2021) · Research Square · doi:10.21203/rs.3.rs-957964/v1 · Cited by 4 · see citing works
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Correia, P. B. C., Baron, E. E., da Silva, J. F. M., & Cortés, O. (2017) · Revista Brasileira de Zootecnia · doi:10.1590/s1806-92902017000200003 · Cited by 3 · see citing works
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Correia, P., Baron, E. E., da Silva, F. M., & Cortés, O. (2014) · PubMed Central · Cited by 2 · see citing works
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- Authors
- Cortés, O., Tupac‐Yupanqui, I., Dunner, S., García‐Atance, M. A., García, D., Fernández, J., & Cañón, J.
- Affiliations
- Universidad Complutense de Madrid (ES)
- Corresponding author
- Cañón, J.
- Venue
- Animal Genetics
- Publisher
- Wiley
- Published
- 2008-11-11
- Volume
- 39
- Issue
- 6
- Pages
- 649-654
- ISSN
- 0268-9146 (ISSN-L)
- 1365-2052
- OpenAlex
- W2027649466
- Cited by
- 32
- References
- 24
- Citations by year
- 2026: 2 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 4 · 2019: 1 · 2018: 1 · 2017: 3 · 2016: 1 · 2015: 2 · 2014: 3 · 2013: 1 · 2012: 2
- FWCI
- 1.52
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Animal Genetics (publishedVersion)
- PubMed (publishedVersion)
- TECNALIA Publications (Fundación TECNALIA Research & Innovation) (submittedVersion, public-domain)
- Funders
- Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
- European Regional Development Fund
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and phenotypic traits in livestock
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Forensic and Genetic Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic diversity and population structure
- Keywords
- mitochondrial DNA, maternal lineage, D-loop, haplotype diversity, African ancestry, European ancestry, haplotype network, Iberian cattle, aurochs
- MeSH terms
- Animals, Base Sequence, Cattle, DNA, Mitochondrial, Haplotypes, Species Specificity, DNA Primers, Genomic Imprinting
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