Characterization of an oxytetracycline‐resistance gene, otrA, of Streptomyces rimosus

Doyle, D.; McDowall, K. J.; Butler, M. J.; Hunter, I. S.

doi:10.1111/j.1365-2958.1991.tb01852.x

Cited by 73Microbial Natural Products and BiosynthesisBacterial Genetics and BiotechnologyAntibiotic Resistance in Bacteria

Characterization of an oxytetracycline‐resistance gene, otrA, of Streptomyces rimosus

Doyle, D., McDowall, K. J., Butler, M. J., Hunter, I. S.·1991·Molecular Microbiology

DOI 10.1111/j.1365-2958.1991.tb01852.x

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Abstract

Summary: The sequence of a 2657 bp DNA fragment containing the coding and regulatory regions of the oxytetracycline (OTC)‐resistance gene, otrA, from the OTC producer Streptomyces rimosus was determined. The predicted amino acid sequence of OtrA had extensive identity with tetracycline‐resistance genes from other bacteria which mediate resistance via non‐covalent ribosomal modification. The N‐terminal domain had extremely high identity with the GTP‐binding sites of elongation factors, such as EF‐G and EF‐Tu, suggesting that binding and hydrolysis of GTP is important to the function of the protein. Significant identity with EF‐G was present throughout the polypeptide. Transcriptional activity upstream of the otrA coding region was investigated. An Escherichia coli‐ type promoter, of otrA p1, was identified. Transcriptional read through of otrA from the upstream gene (otcZ) was also detected in S. rimosus cultures. A divergent promoter activity was identified with sub‐clones of the OtrA fragment in promoter probe vectors analysed in Streptomyces lividans. However, this activity was not identified in a subclone containing more than haif of the otrA coding sequence in S. lividans or at all in S. rimosus, indicating that OtrA negatively regulates the expression of the divergent transcript. The data are consistent with regulation of antibiotic production by OtrA to prevent ‘suicide’.

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Tet(O), like other RPPs, seems to have originated in Streptomyces rimosus, which harbors otrA, an RPP determinant.
“However, Tet(O), like the other RPPs, seems to have originated in the natural producer of oxytetracycline, Streptomyces rimosus , which harbors otrA , an RPP determinant (16, 44, 49).”
From Ribosomal Protection Proteins and Their Mechanism ofTetracyclineResistance (2003) · cites this paper as [16]

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Authors: Doyle, D., McDowall, K. J., Butler, M. J., & Hunter, I. S.
Affiliations: Stanford Medicine (US)
University of Glasgow (GB)
Pfizer (United Kingdom) (GB)
Corresponding author: Hunter, I. S.
ORCID: McDowall, K. J.
Butler, M. J.
Venue: Molecular Microbiology
Publisher: Wiley
Published: 1991-12
Volume: 5
Issue: 12
Pages: 2923-2933
ISSN: 0950-382X (ISSN-L)
1365-2958
DOI: 10.1111/j.1365-2958.1991.tb01852.x
OpenAlex: W2108930122
Cited by: 73
References: 50
Citations by year: 2026: 1 · 2025: 1 · 2024: 1 · 2023: 3 · 2022: 2 · 2021: 3 · 2020: 2 · 2019: 1 · 2018: 7 · 2017: 1 · 2016: 1 · 2015: 2 · 2014: 4 · 2013: 1 · 2012: 3
FWCI: 2.94
Type: journal article
Language: en
Open access: closed
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor, 5444 d)
Locations: Molecular Microbiology (publishedVersion)
PubMed (publishedVersion)
Funders: Abubakar Tafawa Balewa Universty
Division of Electrical, Communications and Cyber Systems
Topics: Health Sciences › Medicine › Pharmacology › Microbial Natural Products and Biosynthesis
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
Keywords: OTRA, oxytetracycline resistance, Streptomyces rimosus, tetracycline resistance, G proteins, elongation factor G, elongation factor Tu, divergent promoters, read-through transcription, antibiotic biosynthesis regulation, suicide prevention, gene expression regulation
MeSH terms: Amino Acid Sequence, Base Sequence, Chromosome Mapping, Cloning, Molecular, Molecular Sequence Data, Oxytetracycline, Peptide Elongation Factors, Promoter Regions, Genetic, Sequence Homology, Nucleic Acid, Streptomyces, Tetracycline Resistance, Transcription, Genetic

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Characterization of an oxytetracycline‐resistance gene, otrA, of Streptomyces rimosus

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