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Transcription at different salinities of Haloferax mediterranei sequences adjacent to partially modified Pst I sites
Abstract
Summary: Two genomic sequences from the halophilic archaeon Haloferax mediterranei , where we had found PstI restriction‐pattern modifications depending on the salinity of the growth medium, have been studied. A markedly salt‐dependent differential expression has been detected in the nearby regions. Two of the open reading frames characterized correspond to two of the differentially expressed transcripts. In both cases the PstI sites were included in purine–pyrimidine alternancies suggestive of Z‐DNA structures and located in non–coding regions with frequent repetitive motifs. A long alternating adenine‐thymine tract also appears in the upstream regions of one of these open reading frames. A possible role of local DNA configuration in osmoregulation in this organism is discussed.
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“A significant breakthrough in understanding the biological function of CRISPR loci occurred with the discovery of Francisco Mojica of the University of Alicante (Spain), who came across similar structures in the archaeal genome of Haloferax mediterranei in 1995 [4].”
From CRISPR–Cas9: A History of Its Discovery and Ethical Considerations of Its Use in Genome Editing (2022) · cites this paper as [4]
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Metadata
- Authors
- Mojica, F. J. M., Juez, G., & Rodriguez‐Valera, F.
- Venue
- Molecular Microbiology
- Publisher
- Wiley
- Published
- 1993-08
- Volume
- 9
- Issue
- 3
- Pages
- 613-621
- ISSN
- 0950-382X
- 1365-2958
- Cited by
- 349
- References
- 0
- Language
- en
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