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Cited by 113Gastrointestinal motility and disordersProbiotics and Fermented FoodsGut microbiota and health

Cell‐free supernatants of Escherichia coli Nissle 1917 modulate human colonic motility: evidence from an in vitro organ bath study

Bär, F., Von Koschitzky, H., Roblick, U., Bruch, H. P., Schulze, L., Sonnenborn, U., Böttner, M., Wedel, T.·2009·Neurogastroenterology & Motility
DOI 10.1111/j.1365-2982.2008.01258.x
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Abstract

Abstract Clinical studies have shown that probiotics influence gastrointestinal motility, e.g. Escherichia coli Nissle 1917 (EcN) (Mutaflor®) proved to be at least as efficacious as lactulose and more potent than placebo in constipated patients. As the underlying mechanisms are not clarified, the effects of EcN culture supernatants on human colonic motility were assessed in vitro. Human colonic circular smooth muscle strips (n = 94, 17 patients) were isometrically examined in an organ bath and exposed to different concentrations of EcN supernatants. Contractility responses were recorded under (i) native conditions, (ii) electrical field stimulation (EFS), (iii) non‐adrenergic non‐cholinergic conditions, and (iv) enteric nerve blockade by tetrodotoxin (TTX). As concentrations of acetic acid were increased in EcN supernatants, contractility responses to acetic acid were additionally tested. EcN supernatants significantly increased the maximal tension forces both at low and high concentrations. Neither blockade of both adrenergic and cholinergic nerves nor application of TTX abolished these effects. EFS‐induced contractility responses were not altered after exposure to EcN supernatants. Acetic acid elicited effects comparable to EcN supernatants only under TTX conditions. EcN supernatants modulate in vitro contractility of the human colon. As neither partial nor TTX blockade of enteric nerves abolished these effects, EcN supernatants appear to enhance colonic contractility by direct stimulation of smooth muscle cells. Active metabolites may include other substances than acetic acid, as acetic acid only partially resembled the effects elicited by EcN supernatants. The data provide a rationale for therapeutical application of probiotics in gastrointestinal motility disorders.

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Authors
Bär, F., Von Koschitzky, H., Roblick, U., Bruch, H. P., Schulze, L., Sonnenborn, U., Böttner, M., & Wedel, T.
Affiliations
  • University of Lübeck (DE)
  • University Hospital Schleswig-Holstein (DE)
  • Ardeypharm (Germany) (DE)
  • Christian-Albrechts-Universität zu Kiel (DE)
Corresponding author
Wedel, T.
ORCID
Wedel, T.
Venue
Neurogastroenterology & Motility
Publisher
Wiley
Published
2009-04-24
Volume
21
Issue
5
Pages
559
ISSN
  • 1350-1925 (ISSN-L)
  • 1365-2982
DOI
10.1111/j.1365-2982.2008.01258.x
OpenAlex
W2149893688
Cited by
113
References
42
Citations by year
2026: 1 · 2025: 3 · 2024: 11 · 2023: 4 · 2022: 7 · 2021: 8 · 2020: 2 · 2019: 5 · 2018: 7 · 2017: 9 · 2016: 11 · 2015: 7 · 2014: 11 · 2013: 8 · 2012: 2
FWCI
6.70
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Neurogastroenterology & Motility (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
Keywords
Escherichia coli Nissle 1917, probiotics, colonic motility, colonic smooth muscle contractility, culture supernatant, acetic acid, enteric nerves, tetrodotoxin, non-adrenergic non-cholinergic transmission, constipation
MeSH terms
Adult, Aged, Aged, 80 and over, Cell-Free System, Colon, Electric Stimulation, Escherichia coli, Female, Gastrointestinal Motility, Humans, Male, Middle Aged, Muscle Contraction, Muscle Relaxation, Tetrodotoxin, Acetic Acid, Probiotics

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