Long‐term potentiation in the rat dentate gyrus is associated with enhanced Arc/Arg3.1 protein expression in spines, dendrites and glia
Abstract
Electron microscopic immunocytochemical methods were used to determine the localization, subcellular distribution and expression of activity-regulated cytoskeletal protein (Arc/Arg3.1) in dentate gyrus after unilateral induction of long-term potentiation (LTP) in the perforant pathway of anaesthetized rats. At 2 h post-induction, immunoreaction product was visible in the dentate gyrus in both the granule cell and molecular layers. Arc expression was higher in the potentiated than the unstimulated contralateral hemisphere. Single-section electron microscopy analysis in unstimulated tissue and in tissue prepared 2 and 4 h after LTP induction showed Arc immunoreactivity (Arc-IR) in dendrites, dendritic spines and glia. Arc-IR was associated with synaptic and non-synaptic plasma membrane apposed to axon terminals and with cytoplasmic organelles, including the cytoskeleton. Arc-IR was also present in neuronal perikarya and there was occasional labelling of nuclei and axons. At 2 h post-LTP induction, there were significant increases in Arc-IR within the granule cell and molecular layers of the dentate gyrus and particularly within the middle molecular layer relative to the inner and outer molecular layers. This increase in Arc expression 2 h after LTP induction was blocked by the N-methyl-D-aspartate receptor antagonist (RS)-3-2-carboxypiperazin-4-yl-propyl-1-phosphonic acid. In animals killed 4 h after LTP induction, Arc expression had declined and differences between the potenti…
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Arc is highly expressed in dendrites, post-synaptic density, and nucleus.
“Arc (also known as Arg3.1), an activity-regulated cytoskeleton-associated protein which belongs to the immediate early gene family, is highly expressed in dendrites910, post-synaptic density (PSD)1112, and nucleus13.”
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- Authors
- Rodríguez, J. J., Davies, H. A., Silva, A. T., De Souza, I. E. J., Peddie, C. J., Colyer, F. M., Lancashire, C. L., Fine, A., Errington, M. L., Bliss, T. V. P., & Stewart, M. G.
- Affiliations
- The Open University (GB)
- National Institute for Medical Research (GB)
- Corresponding author
- Rodríguez, J. J.
- Venue
- European Journal of Neuroscience
- Publisher
- Wiley
- Published
- 2005-05
- Volume
- 21
- Issue
- 9
- Pages
- 2384-2396
- ISSN
- 0953-816X (ISSN-L)
- 1460-9568
- OpenAlex
- W2099829314
- Cited by
- 113
- References
- 0
- Citations by year
- 2025: 1 · 2024: 5 · 2023: 8 · 2022: 4 · 2021: 5 · 2020: 3 · 2019: 1 · 2018: 4 · 2017: 6 · 2016: 2 · 2015: 5 · 2014: 8 · 2013: 9 · 2012: 5
- FWCI
- 3.01
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- European Journal of Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Research Explorer (The University of Manchester) (submittedVersion)
- Funders
- Biotechnology and Biological Sciences Research Council
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
- Keywords
- long-term potentiation, Arc/Arg3.1, dentate gyrus, perforant path, dendritic spines, dendrites, glia, subcellular localization, NMDA receptor, synaptic plasticity, ultrastructure, granule cells, molecular layer
- MeSH terms
- Animals, Axons, Cytoskeletal Proteins, Dendrites, Male, Microscopy, Electron, Nerve Tissue Proteins, Neuroglia, Neuronal Plasticity, Piperazines, Rats, Sprague-Dawley, Long-Term Potentiation, Immediate-Early Proteins, Excitatory Amino Acid Antagonists, Dentate Gyrus, Dendritic Spines, Rats
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