The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.
The galactophilic lectin, LecA, contributes to biofilm development in Pseudomonas aeruginosa
Abstract
Summary: LecA (PA‐IL) is a cytotoxic lectin and adhesin produced by Pseudomonas aeruginosa which binds hydrophobic galactosides with high specificity and affinity. By using a lecA–egfp translation fusion and immunoblot analysis of the biofilm extracellular matrix, we show that lecA is expressed in biofilm‐grown cells. In static biofilm assays on both polystyrene and stainless steel, biofilm depth and surface coverage was reduced by mutation of lecA and enhanced in the LecA‐overproducing strain PAO‐P47. Biofilm surface coverage by the parent strain, PAO‐P47 but not the lecA mutant on steel coupons was also inhibited by growth in the presence of either isopropyl‐β‐D‐thiogalactoside (IPTG) or p ‐nitrophenyl‐α‐D‐galactoside (NPG). Furthermore, mature wild‐type biofilms formed in the absence of these hydrophobic galactosides could be dispersed by the addition of IPTG. In contrast, addition of p ‐nitrophenyl‐α‐L‐fucose (NPF) which has a high affinity for the P. aeruginosa LecB (PA‐IIL) lectin had no effect on biofilm formation or dispersal. Planktonic growth of P. aeruginosa PAO1 was unaffected by the presence of IPTG, NPG or NPF, nor was the strain able to utilize these sugars as carbon sources, suggesting that the observed effects on biofilm formation were due to the competitive inhibition of LecA–ligand binding. Similar results were also obtained for biofilms grown under dynamic flow conditions on steel coupons, suggesting that LecA contributes to P. aeruginosa biofilm architecture under different environmental conditions.
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“Among the most studied are lectins (for example, LecA88 and LecB89) and adhesins (for example, CdrA90), which contribute to biofilm stability and mediate cell–cell, cell–surface and cell–host interactions.”
From Pseudomonas aeruginosa: ecology, evolution, pathogenesis and antimicrobial susceptibility (2025) · cites this paper as [88]
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A modified microtiter-plate test for quantification of staphylococcal biofilm formation
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Communication among Oral Bacteria
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Biofilm formation by Pseudomonas aeruginosa wild type, flagella and type IV pili mutants
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Rhamnolipid Surfactant Production Affects Biofilm Architecture in Pseudomonas aeruginosa PAO1
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Genes involved in matrix formation in Pseudomonas aeruginosa PA14 biofilms
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Cited by
Flemming, H.-C., & Wingender, J. (2010) · Nature Reviews Microbiology · doi:10.1038/nrmicro2415 · Cited by 10,137
Antibiotic resistance in Pseudomonas aeruginosa: mechanisms and alternative therapeutic strategies
Pang, Z., Raudonis, R., Glick, B. R., Lin, T.-J., & Cheng, Z. (2019) · Biotechnology Advances · doi:10.1016/j.biotechadv.2018.11.013 · Cited by 2,306
Pseudomonas aeruginosa Lifestyle: A Paradigm for Adaptation, Survival, and Persistence
Moradali, M. F., Ghods, S., & Rehm, B. H. A. (2017) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2017.00039 · Cited by 1,537
Signals, Regulatory Networks, and Materials That Build and Break Bacterial Biofilms
Karatan, E., & Watnick, P. (2009) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00041-08 · Cited by 1,048
Pseudomonas aeruginosa Biofilms
Thi, M. T. T., Wibowo, D., & Rehm, B. H. A. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21228671 · Cited by 911
Metadata
- Authors
- Diggle, S. P., Stacey, R. E., Dodd, C., Cámara, M., Williams, P., & Winzer, K.
- Venue
- Environmental Microbiology
- Publisher
- Wiley
- Published
- 2006-02-16
- Volume
- 8
- Issue
- 6
- Pages
- 1095-1104
- ISSN
- 1462-2912 (ISSN-L)
- 1462-2920
- OpenAlex
- W2134440330
- Cited by
- 375
- References
- 44
- FWCI
- 3.19
- Type
- journal article
- Language
- en
- Open access
- closed
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- Diggle, S. P., Stacey, R. E., Dodd, C., Cámara, M., Williams, P., & Winzer, K. (2006). The galactophilic lectin, LecA, contributes to biofilm development in Pseudomonas aeruginosa. Environmental Microbiology. https://doi.org/10.1111/j.1462-2920.2006.001001.x
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