The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.
Culture‐independent analysis of the gut microbiota in colorectal cancer and polyposis
Abstract
Summary: A role for the intestinal microbiota is routinely cited as a potential aetiological factor in colorectal cancer initiation and progression. As the majority of bacteria in the gut are refractory to culture we investigated this ecosystem in subjects with colorectal cancer and with adenomatous polyposis who are at high risk of developing colorectal cancer, using culture‐independent methods. Twenty colorectal cancer and 20 polypectomized volunteers were chosen for this analysis. An exploration of the diversity and temporal stability of the dominant bacteria and several bacterial subgroups was undertaken using 16S rRNA gene denaturing gradient gel electrophoresis and ribosomal intergenic spacer analysis (RISA). Metabonomic analysis of the distal gut microbiota's environment was also undertaken. A significantly reduced temporal stability and increased diversity for the microbiota of subjects with colorectal cancer and polyposis was evident. A significantly increased diversity of the Clostridium leptum and C. coccoides subgroups was also noted for both disease groups. A clear division in the metabonome was observed for the colorectal cancer and polypectomized subjects compared with control volunteers. The intestinal microbiota and their metabolites are significantly altered in both colorectal cancer and polypectomized subjects compared with controls.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“An increased diversity in Clostridium leptum (C.leptum) and Clostridium coccoides is present, while temporary instability and decreased complexity in composition are linked to colitis-associated CRC [48,49] .”
From Intestinal microbiota in health and disease: Role of bifidobacteria in gut homeostasis (2014) · cites this paper as [48]
In the citing paper
“Reduced temporal stability and increased diversity has been shown for the faecal microbiota of subjects with established CRC and polyposis,86”
From The gut microbiota and host health: a new clinical frontier (2016) · cites this paper as [86]
Citations
Cites
Diversity of the Human Intestinal Microbial Flora
Eckburg, P. B., Bik, E. M., Bernstein, C. N., Purdom, E., Dethlefsen, L., Sargent, M., Gill, S. R., Nelson, K. E., & Relman, D. A. (2005) · Science · doi:10.1126/science.1110591 · Cited by 7,913
Biodiversity and ecosystem functioning: Current knowledge and future challenges
Loreau, M., Naeem, S., Inchausti, P., Bengtsson, J., Grime, J. P., Hector, A., Hooper, D. U., Huston, M. A., Raffaelli, D., Schmid, B., et al. (2001) · Science · doi:10.1126/science.1064088 · Cited by 4,649
Genetic instabilities in human cancers
Lengauer, C., Kinzler, K. W., & Vogelstein, B. (1998) · Nature · doi:10.1038/25292 · Cited by 4,168 · cited 2×
“The majority (~90%) of colorectal cancers arise sporadically in a time-dependent manner through adenomatous polyp formation with the activation of oncogenes such as K-ras (Lengauer et al., 1998) and inactivation of tumour suppressor genes such as APC and p53 (Hope et al.”
BackgroundMLEV-17-based two-dimensional homonuclear magnetization transfer spectroscopy
Bax, A., & Davis, D. G. (1985) · Journal of Magnetic Resonance (1969) · doi:10.1016/0022-2364(85)90018-6 · Cited by 3,605
Development of the Human Infant Intestinal Microbiota
Palmer, C., Bik, E. M., DiGiulio, D. B., Relman, D. A., & Brown, P. O. (2007) · PLoS Biology · doi:10.1371/journal.pbio.0050177 · Cited by 2,877
MICROBIAL ECOLOGY OF THE GASTROINTESTINAL TRACT
Savage, D. C. (1977) · Annual Review of Microbiology · doi:10.1146/annurev.mi.31.100177.000543 · Cited by 2,436
Reduced diversity of faecal microbiota in Crohn’s disease revealed by a metagenomic approach
Manichanh, C., Rigottier-Gois, L., Bonnaud, E., Gloux, K., Pelletier, E., Frangeul, L., Nalin, R., Jarrin, C., Chardon, P., Marteau, P., et al. (2006) · Gut · doi:10.1136/gut.2005.073817 · Cited by 2,277
Suau, A., Bonnet, R., Sutren, M., Godon, J.-J., Gibson, G. R., Collins, M. D., & Doré, J. (1999) · Applied and Environmental Microbiology · doi:10.1128/aem.65.11.4799-4807.1999 · Cited by 1,490
Nübel, U., Engelen, B., Felske, A., Snaidr, J., Wieshuber, A., Amann, R., Ludwig, W., & Backhaus, H. (1996) · Journal of Bacteriology · doi:10.1128/jb.178.19.5636-5643.1996 · Cited by 1,356
The microbiology of butyrate formation in the human colon
Pryde, S. E., Duncan, S. H., Hold, G. L., Stewart, C. S., & Flint, H. J. (2002) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2002.tb11467.x · Cited by 1,320
Cited by
Sorted by citations
Gut microbiota in health and disease
Sekirov, I., Russell, S. L., Antunes, L. C. M., & Finlay, B. B. (2010) · Physiological Reviews · doi:10.1152/physrev.00045.2009 · Cited by 4,182
“Several recent studies have used RISA to identify the enhanced colonization of certain bacterial species in patients with diseases like colorectal cancer (265) and IBD (151) compared with healthy controls.”
BackgroundResultInfluentialKostic, A. D., Chun, E., Robertson, L., Glickman, J. N., Gallini, C. A., Michaud, M., Clancy, T. E., Chung, D. C., Lochhead, P., Hold, G. L., et al. (2013) · Cell Host & Microbe · doi:10.1016/j.chom.2013.07.007 · Cited by 2,718
“A few recent studies have performed case control studies of colorectal adenomas using 16S rRNA gene surveys with one noting fusobacterial enrichments in their patient cohort (McCoy et al., 2013; Sanapareddy et al., 2012; Scanlan et al., 2008; Shen et al., 2010).”
BackgroundThe gut microbiota and host health: a new clinical frontier
Marchesi, J. R., Adams, D. H., Fava, F., Hermes, G. D. A., Hirschfield, G. M., Hold, G., Quraishi, M. N., Kinross, J., Smidt, H., Tuohy, K. M., et al. (2016) · Gut · doi:10.1136/gutjnl-2015-309990 · Cited by 2,302
“…CRC. 85 Reduced temporal stability and increased diversity has been shown for the faecal microbiota of subjects with established CRC and polyposis, 86 and now metagenomic and metatranscriptomic studies have identi fi ed an individualised oncogenic microbiome and speci fi c bacterial species that…”
BackgroundSelf-citationTerzić, J., Grivennikov, S., Karin, E., & Karin, M. (2010) · Gastroenterology · doi:10.1053/j.gastro.2010.01.058 · Cited by 1,974
Yu, J. Q., Feng, Q., Wong, S. H., Zhang, D., Liang, Q. Y., Qin, Y., Tang, L., Zhao, H., Stenvang, J., Li, Y., et al. (2015) · Gut · doi:10.1136/gutjnl-2015-309800 · Cited by 1,273
Metadata
- Authors
- Scanlan, P. D., Shanahan, F., Clune, Y., Collins, J. K., O'Sullivan, G. C., O'Riordan, M., Holmes, E., Wang, Y., & Marchesi, J. R.
- Affiliations
- University College Cork (IE)
- Imperial College London (GB)
- Corresponding author
- Marchesi, Julian R.
- ORCID
- Venue
- Environmental Microbiology
- Publisher
- Wiley
- Published
- 2008-01-25
- Volume
- 10
- Issue
- 3
- Pages
- 789-798
- ISSN
- 1462-2912 (ISSN-L)
- 1462-2920
- OpenAlex
- W2146480823
- Cited by
- 245
- References
- 47
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 4 · 2023: 6 · 2022: 8 · 2021: 12 · 2020: 10 · 2019: 14 · 2018: 20 · 2017: 13 · 2016: 17 · 2015: 23 · 2014: 22 · 2013: 26 · 2012: 12
- FWCI
- 6.96
- Type
- journal article
- Language
- en
- Update notices
- erratum (2008-04-04)
- Open access
- closed
- Locations
- Environmental Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Health Research Board
- Higher Education Authority
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Medicine › Infectious Diseases › Clostridium difficile and Clostridium perfringens research
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Keywords
- gut microbiota, colorectal cancer, adenomatous polyposis, polypectomy, microbial diversity, temporal stability, Clostridium leptum, Clostridium coccoides, 16S rRNA gene, RISA, metabolomics, cancer etiology
- MeSH terms
- Clostridium, DNA, Bacterial, Electrophoresis, Polyacrylamide Gel, Female, Humans, Intestines, Male, Adenomatous Polyposis Coli, RNA, Ribosomal, 16S, Colorectal Neoplasms, DNA, Ribosomal Spacer, Gastrointestinal Tract, Biodiversity
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
