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Cited by 229Ion channel regulation and functionNeuroscience and Neuropharmacology ResearchIon Transport and Channel Regulation

Functional differences in Na+ channel gating between fast‐spiking interneurones and principal neurones of rat hippocampus

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Martina, Marco, Jonas, Peter·1997·The Journal of Physiology
DOI 10.1111/j.1469-7793.1997.593ba.xPMC PMC1160038
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Abstract

1. GABAergic interneurones differ from glutamatergic principal neurones in their ability to discharge high-frequency trains of action potentials without adaptation. To examine whether Na+ channel gating contributed to these differences, Na+ currents were recorded in nucleated patches from interneurones (dentate gyrus basket cells, BCs) and principal neurones (CA1 pyramidal cells, PCs) of rat hippocampal slices. 2. The voltage dependence of Na+ channel activation in BCs and PCs was similar. The slope factors of the activation curves, fitted with Boltzmann functions raised to the third power, were 11.5 and 11.8 mV, and the mid-point potentials were -25.1 and -23.9 mV, respectively. 3. Whereas the time course of Na+ channel activation (-30 to +40 mV) was similar, the deactivation kinetics (-100 to -40 mV) were faster in BCs than in PCs (tail current decay time constants, 0.13 and 0.20 ms, respectively, at -40 mV). 4. Na+ channels in BCs and PCs differed in the voltage dependence of inactivation. The slope factors of the steady-state inactivation curves fitted with Boltzmann functions were 6.7 and 10.7 mV, and the mid-point potentials were -58.3 and -62.9 mV, respectively. 5. The onset of Na+ channel inactivation at -55 mV was slower in BCs than in PCs; the inactivation time constants were 18.6 and 9.3 ms, respectively. At more positive potentials the differences in inactivation onset were smaller. 6. The time course of recovery of Na+ channels from inactivation induced by a 30 ms pulse was fast and mono-exponential (tau = 2.0 ms at -120 mV) in BCs, whereas it was slower and bi-exponential in PCs (tau 1 = 2.0 ms and tau 2 = 133 ms; amplitude contribution of the slow component, 15%). 7. We conclude that Na+ channels of BCs and PCs differ in gating properties that contribute to the characteristic action potential patterns of the two types of neurones.

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Authors
Martina, M., & Jonas, P.
Affiliations
University of Freiburg (DE)
ORCID
Martina, Marco
Venue
The Journal of Physiology
Publisher
Wiley
Published
1997-12
Volume
505
Issue
3
Pages
593-603
ISSN
  • 0022-3751 (ISSN-L)
  • 1469-7793
DOI
10.1111/j.1469-7793.1997.593ba.x
PMC
PMC1160038
OpenAlex
W2080803688
Cited by
229
References
41
Citations by year
2026: 2 · 2025: 2 · 2024: 2 · 2023: 7 · 2022: 2 · 2021: 6 · 2020: 3 · 2019: 9 · 2018: 8 · 2017: 5 · 2016: 8 · 2015: 8 · 2014: 12 · 2013: 2 · 2012: 7
FWCI
2.13
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 2494 d)
Locations
  • The Journal of Physiology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • FreiDok plus (Universitätsbibliothek Freiburg) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
Keywords
sodium channel gating, fast-spiking interneurons, basket cells, CA1 pyramidal neurons, hippocampus, GABAergic interneurons, glutamatergic neurons, sodium channel inactivation, recovery from inactivation, high-frequency firing, voltage dependence of activation, deactivation kinetics
MeSH terms
Action Potentials, Animals, gamma-Aminobutyric Acid, Hippocampus, Interneurons, Time Factors, Sodium Channels, Ion Channel Gating, Rats, Wistar, Pyramidal Cells, Patch-Clamp Techniques, Dentate Gyrus, Rats, In Vitro Techniques

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