Characterization of the high affinity Zn transporter from Noccaea caerulescens, NcZNT1, and dissection of its promoter for its role in Zn uptake and hyperaccumulation

Milner, Matthew J.; Craft, Eric; Yamaji, Naoki; Koyama, Emi; Ma, Jian Feng; Kochian, Leon V.

doi:10.1111/j.1469-8137.2012.04144.x

Cited by 73Plant Micronutrient Interactions and EffectsHeavy metals in environmentHeavy Metals in Plants

Characterization of the high affinity Zn transporter from Noccaea caerulescens, NcZNT1, and dissection of its promoter for its role in Zn uptake and hyperaccumulation

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Milner, Matthew J., Craft, Eric, Yamaji, Naoki, Koyama, Emi, Ma, Jian Feng, Kochian, Leon V.·2012·New Phytologist

DOI 10.1111/j.1469-8137.2012.04144.x

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Abstract

• In this paper, we conducted a detailed analysis of the ZIP family transporter, NcZNT1, in the zinc (Zn)/cadmium (Cd) hyperaccumulating plant species, Noccaea caerulescens, formerly known as Thlaspi caerulescens. NcZNT1 was previously suggested to be the primary root Zn/Cd uptake transporter. Both a characterization of NcZNT1 transport function in planta and in heterologous systems, and an analysis of NcZNT1 gene expression and NcZNT1 protein localization were carried out. • We show that NcZNT1 is not only expressed in the root epidermis, but also is highly expressed in the root and shoot vasculature, suggesting a role in long-distance metal transport. Also, NcZNT1 was found to be a plasma membrane transporter that mediates Zn but not Cd, iron (Fe), manganese (Mn) or copper (Cu) uptake into plant cells. • Two novel regions of the NcZNT1 promoter were identified which may be involved in both the hyperexpression of NcZNT1 and its ability to be regulated by plant Zn status. • In conclusion, we demonstrate here that NcZNT1 plays a role in Zn and not Cd uptake from the soil, and based on its strong expression in the root and shoot vasculature, could be involved in long-distance transport of Zn from the root to the shoot via the xylem.

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Upon exposure to Zn deficient soil, a tissue-specific expression pattern of NcZNT1 was observed in N. caerulescens.
“Upon exposure to Zn deficient soil, a tissue-specific expression pattern of NcZNT1 was observed in N. caerulescens (Milner et al., 2012).”
From Plant hyperaccumulators: a state-of-the-art review on mechanism of heavy metal transport and sequestration (2025) · cites this paper as Milner et al., 2012
The Relative expression of NcZNT1 section reports different transcript levels in roots and shoots, and the protein-localization results show a distinct pattern within N. caerulescens roots under Zn deficiency. However, the Immunohistological staining methods say the plants were exposed to Zn-deficient nutrient solution, not soil. (LLM, conf=0.88)
Experimental — accuracy not yet measured
NcZNT1 was differentially expressed in shoot apical meristem and mesophyll, bundle sheath, and stomatal guard cells.
“In addition, it is reported that NcZNT1 was differentially expressed in shoot apical meristem and mesophyll, bundle sheath, and stomatal guard cells.”
From Plant hyperaccumulators: a state-of-the-art review on mechanism of heavy metal transport and sequestration (2025) · cites this paper as Milner et al., 2012
The tissue-specific localization passage (under “NcZNT1 metal transport properties”; page unavailable) reports shoot expression in leaf guard cells and the shoot apical meristem. It does not report expression in mesophyll or bundle sheath cells, so the full list is not supported by this paper. (LLM, conf=0.87)
Experimental — accuracy not yet measured
Tissue-specific expression was identified for expression of NcZNT1 in stellar parenchyma cells, pericycle, and very low expression in cortex and rhizodermis in N. caerulescens.
“Moreover, tissue-specific expression was identified for expression of NcZNT1 in stellar parenchyma cells, pericycle, and very low expression in cortex and rhizodermis in N. caerulescens (Milner et al., 2012).”
From Plant hyperaccumulators: a state-of-the-art review on mechanism of heavy metal transport and sequestration (2025) · cites this paper as Milner et al., 2012
NcZNT5 expression was limited to young leaves, especially its epidermal cells.
“On the contrary, NcZNT5 expression was limited to young leaves, especially its epidermal cells (Küpper and Kochian, 2010; Milner et al., 2012).”
From Plant hyperaccumulators: a state-of-the-art review on mechanism of heavy metal transport and sequestration (2025) · cites this paper as Milner et al., 2012
The tissue-localization and relative-expression sections (page not provided) discuss NcZNT1, not NcZNT5. Searches for NcZNT5 and its expression found no passage in our copy addressing whether it is limited to young leaves or their epidermal cells. (LLM, conf=0.75)
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Expression of the ZNT1 Zinc Transporter from the Metal Hyperaccumulator Noccaea caerulescens Confers Enhanced Zinc and Cadmium Tolerance and Accumulation to Arabidopsis thaliana
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The molecular physiology of heavy metal transport in the Zn/Cd\n hyperaccumulator Thlaspi caerulescens
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Summary
Milner, M. J., Craft, E. J., Yamaji, N., Koyama, E., Ma, J. F., Kochian, L. V., & Kochian, L. V. (2012)
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An evolutionary perspective on differential regulation of zinc and cadmium homeostatis genes in Arabidopsis thaliana and Noccaea caerulescens
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Authors: Milner, M. J., Craft, E., Yamaji, N., Koyama, E., Ma, J. F., & Kochian, L. V.
Affiliations: United States Department of Agriculture (US)
Okayama University (JP)
Corresponding author: Kochian, Leon V.
ORCID: Milner, Matthew J.
Craft, Eric
Yamaji, Naoki
Kochian, Leon V.
Venue: New Phytologist
Publisher: Wiley
Published: 2012-04-23
Volume: 195
Issue: 1
Pages: 113-123
ISSN: 0028-646X (ISSN-L)
1469-8137
DOI: 10.1111/j.1469-8137.2012.04144.x
OpenAlex: W2032097686
Cited by: 73
References: 28
Citations by year: 2026: 2 · 2025: 6 · 2024: 4 · 2023: 3 · 2022: 9 · 2021: 10 · 2020: 8 · 2019: 5 · 2018: 5 · 2017: 2 · 2016: 3 · 2015: 1 · 2014: 4 · 2013: 9 · 2012: 2
FWCI: 5.83
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor)
doi.wiley.com/10.1002/tdm_license_1.1 (tdm, 1226 d)
Locations: New Phytologist (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Micronutrient Interactions and Effects
Physical Sciences › Environmental Science › Pollution › Heavy metals in environment
Physical Sciences › Chemistry › Analytical Chemistry › Heavy Metals in Plants
Keywords: Noccaea caerulescens, Thlaspi caerulescens, zinc uptake, cadmium hyperaccumulation, promoter analysis, plasma membrane transporters, xylem transport, metal hyperaccumulation, plant metal homeostasis
MeSH terms: Cadmium, Carrier Proteins, Copper, Manganese, Plant Proteins, Promoter Regions, Genetic, Zinc, Arabidopsis, Sequence Deletion, Gene Expression Regulation, Plant, Plant Roots, Plant Shoots, Brassicaceae, Cation Transport Proteins, Plants, Genetically Modified, Plant Cells

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Characterization of the high affinity Zn transporter from Noccaea caerulescens, NcZNT1, and dissection of its promoter for its role in Zn uptake and hyperaccumulation

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