Arabidopsis thaliana MTP1 is a Zn transporter in the vacuolar membrane which mediates Zn detoxification and drives leaf Zn accumulation
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The Arabidopsis thaliana metal tolerance protein 1 (MTP1) of the cation diffusion facilitator family of membrane transport proteins can mediate the detoxification of Zn in Arabidopsis and yeast. Xenopus laevis oocytes expressing AtMTP1 accumulate more Zn than oocytes expressing the AtMTP1(D94A) mutant or water-injected oocytes. An AtMTP1-GFP fusion protein localizes to the vacuolar membrane in root and leaf cells. The analysis of Arabidopsis transformed with a promoter-GUS construct suggests that AtMTP1 is not produced throughout the plant, but primarily in the subpopulation of dividing, differentiating and expanding cells. RNA interference-mediated silencing of AtMTP1 causes Zn hypersensitivity and a reduction in Zn concentrations in vegetative plant tissues.
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Expression analysis and RNAi-produced lines identified tissue-specific expression of NgMTP1 encoding MTP1 to accumulate Zn in shoots of hyperaccumulators.
“Expression analysis and RNAi-produced lines identified tissue-specific expression of NgMTP1encoding MTP1 to accumulate Zn in shoots of hyperaccumulators (Desbrosses-Fonrouge et al., 2005).”
From Plant hyperaccumulators: a state-of-the-art review on mechanism of heavy metal transport and sequestration (2025) · cites this paper as Desbrosses-Fonrouge et al., 2005
Section 3.5 reports that RNAi suppression of AtMTP1 reduced Zn concentrations in Arabidopsis thaliana leaves and stems. Section 3.3 examines AtMTP1 expression across tissues, but the paper does not establish tissue-specific NgMTP1 expression or an NgMTP1 role in shoot Zn accumulation in hyperaccumulators. The Discussion (section 4) explicitly presents the role of MTP1 genes in hyperaccumulator leaf Zn accumulation as a question for future investigation; page numbers are unavailable in our copy. (LLM, conf=0.88)
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Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961 · see citing works
Towbin, H., Staehelin, T., & Gordon, J. (1979) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.76.9.4350 · Cited by 51,421 · see citing works
Floral dip: a simplified method for Agrobacterium ‐mediated transformation of Arabidopsis thaliana
Clough, S. J., & Bent, A. F. (1998) · The Plant Journal · doi:10.1046/j.1365-313x.1998.00343.x · Cited by 22,014 · see citing works
Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data
Ramakers, C. R. B., Ruijter, J. M., Deprez, R. H. L., & Moorman, A. F. M. (2003) · Neuroscience Letters · doi:10.1016/s0304-3940(02)01423-4 · Cited by 3,718 · see citing works
GATEWAY™ vectors for Agrobacterium-mediated plant transformation
Karimi, M., Inzé, D., & Depicker, A. (2002) · Trends in Plant Science · doi:10.1016/s1360-1385(02)02251-3 · Cited by 3,639 · see citing works
Haseloff, J. P., Siemering, K. R., Prasher, D. C., & Hodge, S. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.6.2122 · Cited by 1,322 · see citing works
Phylogenetic Relationships within Cation Transporter Families of Arabidopsis
Mäser, P., Thomine, S., Schroeder, J. I., Ward, J. M., Hirschi, K. D., Sze, H., Talke, I. N., Anna Amtmann, A., Maathuis, F. J. M., Sanders, D., et al. (2001) · PLANT PHYSIOLOGY · doi:10.1104/pp.126.4.1646 · Cited by 1,257 · see citing works
Self-citationSubunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs
Liman, E. R., Tytgat, J., & Hess, P. (1992) · Neuron · doi:10.1016/0896-6273(92)90239-a · Cited by 1,072 · see citing works
Hambidge, M. (2000) · Journal of Nutrition · doi:10.1093/jn/130.5.1344s · Cited by 785 · see citing works
Grotz, N., Fox, T. C., Connolly, E. L., Park, W. D., Guerinot, M. L., & Eide, D. J. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.12.7220 · Cited by 735 · see citing works
Czechowski, T., Bari, R. P., Stitt, M., Scheible, W., & Udvardi, M. K. (2004) · The Plant Journal · doi:10.1111/j.1365-313x.2004.02051.x · Cited by 621 · see citing works
Becher, M., Talke, I. N., Krall, L., & Krämer, U. (2004) · The Plant Journal · doi:10.1046/j.1365-313x.2003.01959.x · Cited by 568 · see citing works
Self-citationZhao, H. F., & Eide, D. J. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.6.2454 · Cited by 566 · see citing works
Identification of SLC39A4, a gene involved in acrodermatitis enteropathica
Küry, S., Dréno, B., Bezieau, S., Giraudet, S., Kharfi, M., Kamoun, R., & Moisan, J. (2002) · Nature Genetics · doi:10.1038/ng913 · Cited by 531 · see citing works
Eukaryotic zinc transporters and their regulation
Gaither, L. A., & Eide, D. J. (2001) · BioMetals · doi:10.1023/a:1012988914300 · Cited by 526 · see citing works
The vacuolar membrane protein gamma‐TIP creates water specific channels in Xenopus oocytes.
Maurel, C., Reizer, J., Schroeder, J. I., et al. (1993) · The EMBO Journal · doi:10.1002/j.1460-2075.1993.tb05877.x · Cited by 520 · see citing works
A novel member of a zinc transporter family is defective in acrodermatitis enteropathica
Wang, K., Zhou, B., Kuo, Y.-M., Zemansky, J., & Gitschier, J. (2002) · The American Journal of Human Genetics · doi:10.1086/341125 · Cited by 491 · see citing works
AtNRAMP3, a multispecific vacuolar metal transporter involved in plant responses to iron deficiency
Thomine, S., Lelièvre, F., Debarbieux, E., Schroeder, J. I., & Barbier‐Brygoo, H. (2003) · The Plant Journal · doi:10.1046/j.1365-313x.2003.01760.x · Cited by 480 · see citing works
Self-citationHenri Wintz, A., Fox, T. C., Wu, Y., Feng, V., Chen, W., Chang, H., Zhu, T., & Vulpe, C. D. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m309338200 · Cited by 431 · see citing works
van der Zaal, B. J., Neuteboom, L. W., Pinas, J. E., Chardonnens, A. N., Schat, H., Verkleij, J. A. C., & Hooykaas, P. J. J. (1999) · PLANT PHYSIOLOGY · doi:10.1104/pp.119.3.1047 · Cited by 415 · see citing works
The Gene Encodes the Low Affinity Zinc Transporter in
Zhao, H. F., & Eide, D. J. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.38.23203 · Cited by 396 · see citing works
A Novel Family of Ubiquitous Heavy Metal Ion Transport Proteins
Paulsen, I. T., & Saier, M. H. (1997) · The Journal of Membrane Biology · doi:10.1007/s002329900192 · Cited by 393 · see citing works
Zinc transporters that regulate vacuolar zinc storage in Saccharomyces cerevisiae
MacDiarmid, C. W., Gaither, L. A., & Eide, D. (2000) · The EMBO Journal · doi:10.1093/emboj/19.12.2845 · Cited by 389 · see citing works
Efflux and compartmentalization of zinc by members of the SLC30 family of solute carriers
Palmiter, R. D., & Huang, L. (2004) · Pflügers Archiv - European Journal of Physiology · doi:10.1007/s00424-003-1070-7 · Cited by 384 · see citing works
Soluble, highly fluorescent variants of green fluorescent protein (GFP) for use in higher plants
Davis, S. J., & Vierstra, R. D. (1998) · Plant Molecular Biology · doi:10.1023/a:1005991617182 · Cited by 382 · see citing works
Coleman, J. E. (1998) · Current Opinion in Chemical Biology · doi:10.1016/s1367-5931(98)80064-1 · Cited by 362 · see citing works
Functional Expression of the Human hZIP2 Zinc Transporter
Gaither, L. A., & Eide, D. J. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.8.5560 · Cited by 328 · see citing works
Constructs and methods for high-throughput gene silencing in plants
Helliwell, C. A. (2003) · Methods · doi:10.1016/s1046-2023(03)00036-7 · Cited by 320 · see citing works
Persans, M. W., Nieman, K., & Salt, D. E. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.171039798 · Cited by 317 · see citing works
Kobae, Y., Uemura, T., Sato, M. H., Ohnishi, M., Mimura, T., Nakagawa, T., & Maeshima, M. (2004) · Plant and Cell Physiology · doi:10.1093/pcp/pci015 · Cited by 311 · see citing works
Dräger, D. B., Desbrosses‐Fonrouge, A., Krach, C., Chardonnens, A. N., Meyer, R. C., Saumitou‐Laprade, P., & Krämer, U. (2004) · The Plant Journal · doi:10.1111/j.1365-313x.2004.02143.x · Cited by 294 · see citing works
Self-citationGenes Encoding Proteins of the Cation Diffusion Facilitator Family That Confer Manganese Tolerance
Delhaize, E., Kataoka, T., Hebb, D. M., White, R. G., & Ryan, P. R. (2003) · The Plant Cell · doi:10.1105/tpc.009134 · Cited by 261 · see citing works
Binary vectors which allow the exchange of plant selectable markers and reporter genes
Becker, D. (1990) · Nucleic Acids Research · doi:10.1093/nar/18.1.203 · Cited by 246 · see citing works
COT1, a Gene Involved in Cobalt Accumulation in Saccharomyces cerevisiae
Conklin, D. S., Mcmaster, J. A., Culbertson, M. R., & Kung, C. (1992) · Molecular and Cellular Biology · doi:10.1128/mcb.12.9.3678-3688.1992 · Cited by 215 · see citing works
Gitan, R. S., Luo, H. M., Rodgers, J., Broderius, M. A., & Eide, D. J. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.44.28617 · Cited by 205 · see citing works
Biochemical Properties of Vacuolar Zinc Transport Systems ofSaccharomyces cerevisiae
MacDiarmid, C. W., Milanick, M. A., & Eide, D. J. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m205052200 · Cited by 203 · see citing works
Kamizono, A., Nishizawa, M., Teranishi, Y., Murata, K., & Kimura, A. (1989) · Molecular and General Genetics MGG · doi:10.1007/bf00261172 · Cited by 201 · see citing works
Blaudez, D., Kohler, A., Martin, F., Sanders, D., & Chalot, M. (2003) · The Plant Cell · doi:10.1105/tpc.017541 · Cited by 200 · see citing works
Zinc Fingers in Caenorhabditis elegans : Finding Families and Probing Pathways
Clarke, N. D., & Berg, J. M. (1998) · Science · doi:10.1126/science.282.5396.2018 · Cited by 198 · see citing works
Induction of the ZRC1 Metal Tolerance Gene in Zinc-limited Yeast Confers Resistance to Zinc Shock
MacDiarmid, C. W., Milanick, M. A., & Eide, D. J. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m300568200 · Cited by 189 · see citing works
Kinetic Study of the Antiport Mechanism of an Escherichia coli Zinc Transporter, ZitB
Chao, Y., & Fu, D. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m313510200 · Cited by 165 · see citing works
Kim, B., Wang, F., Dufner‐Beattie, J., et al. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m310799200 · Cited by 153 · see citing works
Guffanti, A. A., Wei, Y., Rood, S. V., & KRULWICH, T. A. (2002) · Molecular Microbiology · doi:10.1046/j.1365-2958.2002.02998.x · Cited by 150 · see citing works
Kim, D., Gustin, J. L., Lahner, B., Persans, M. W., Baek, D., Yun, D., & Salt, D. E. (2004) · The Plant Journal · doi:10.1111/j.1365-313x.2004.02126.x · Cited by 148 · see citing works
Zinc-stimulated Endocytosis Controls Activity of the Mouse ZIP1 and ZIP3 Zinc Uptake Transporters
Wang, F., Dufner‐Beattie, J., Kim, B., Petris, M. J., Andrews, G. K., & Eide, D. J. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m400680200 · Cited by 133 · see citing works
Bloß, T., Clemens, S., & Nies, D. H. (2002) · Planta · doi:10.1007/s00425-001-0677-1 · Cited by 108 · see citing works
Zinc physiology and biochemistry in oocytes and embryos
Falchuk, K. H., & Montorzi, M. (2001) · BioMetals · doi:10.1023/a:1012994427351 · Cited by 86 · see citing works
Wycisk, K. A., Kim, E. J., Schroeder, J. I., & Krämer, U. (2004) · FEBS Letters · doi:10.1016/j.febslet.2004.10.086 · Cited by 82 · see citing works
Self-citation
Flagged first, then by citations
Research works printed but not linked: 2
- Woolhouse, H.W. (1983) (Lange, O.L., Nobel, P.S., Osmond, C.B. and Ziegler, H., Eds.), Encyclopedia of Plant Physiology: Responses to the Chemical and Biological Environment, Vol. sNew Series Vol. 12 C, Springer Verlag, Berlin.
- Sambrook, J. and Russel, D.W. (2001) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
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Eide, D. J. (2006) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2006.03.005 · Cited by 874 · see citing works
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Hanikenne, M., Talke, I. N., Haydon, M. J., Lanz, C., Nolte, A., Motte, P., Kroymann, J., Weigel, D., & Krämer, U. (2008) · Nature · doi:10.1038/nature06877 · Cited by 812 · see citing works
Self-citationKrämer, U., Talke, I. N., & Hanikenne, M. (2007) · FEBS Letters · doi:10.1016/j.febslet.2007.04.010 · Cited by 583 · see citing works
Self-citationTalke, I. N., Hanikenne, M., & Krämer, U. (2006) · Plant Physiology · doi:10.1104/pp.105.076232 · Cited by 452 · see citing works
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Andresen, E., Peiter, E., & Küpper, H. (2018) · Journal of Experimental Botany · doi:10.1093/jxb/erx465 · Cited by 431 · see citing works
A secretory pathway-localized cation diffusion facilitator confers plant manganese tolerance
Peiter, E., Montanini, B., Gobert, A., Pedas, P., Husted, S., Maathuis, F. J. M., Blaudez, D., Chalot, M., & Sanders, D. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0609507104 · Cited by 287 · see citing works
Gustin, J. L., Loureiro, M. E., Kim, D., Na, G., Tikhonova, M., & Salt, D. E. (2009) · The Plant Journal · doi:10.1111/j.1365-313x.2008.03754.x · Cited by 203 · see citing works
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Küpper, H., & Kochian, L. V. (2010) · New Phytologist · doi:10.1111/j.1469-8137.2009.03051.x · Cited by 191 · see citing works
Shahzad, Z., Gosti, F., Frérot, H., Lacombe, E., Roosens, N., Saumitou-Laprade, P., & Berthomieu, P. (2010) · PLoS Genetics · doi:10.1371/journal.pgen.1000911 · Cited by 124 · see citing works
Milner, M. J., Craft, E., Yamaji, N., Koyama, E., Ma, J. F., & Kochian, L. V. (2012) · New Phytologist · doi:10.1111/j.1469-8137.2012.04144.x · Cited by 73 · see citing works
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- Authors
- Desbrosses-Fonrouge, A.-G., Voigt, K., Schröder, A., Arrivault, S., Thomine, S., & Krämer, U.
- Affiliations
- Max Planck Society (DE)
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Krämer, Ute
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 2005-07-06
- Volume
- 579
- Issue
- 19
- Pages
- 4165-4174
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2024264388
- Cited by
- 305
- References
- 48
- Citations by year
- 2026: 11 · 2025: 20 · 2024: 14 · 2023: 13 · 2022: 18 · 2021: 25 · 2020: 22 · 2019: 14 · 2018: 17 · 2017: 10 · 2016: 13 · 2015: 7 · 2014: 16 · 2013: 15 · 2012: 14
- FWCI
- 14.83
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Funders
- Deutscher Akademischer Austauschdienst
- Deutsche Forschungsgemeinschaft
- Bundesministerium für Bildung und Forschung
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Micronutrient Interactions and Effects
- Health Sciences › Nursing › Nutrition and Dietetics › Trace Elements in Health
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Keywords
- zinc transporters, tonoplast, zinc detoxification, cation diffusion facilitator, Arabidopsis thaliana, subcellular localization, RNA interference, zinc homeostasis
- MeSH terms
- Base Sequence, Biological Transport, Cloning, Molecular, Intracellular Membranes, Vacuoles, Zinc, Arabidopsis, DNA Primers, Plant Leaves, Reverse Transcriptase Polymerase Chain Reaction, Cation Transport Proteins, Arabidopsis Proteins
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