Arabidopsis thaliana transcription factors bZIP19 and bZIP23 regulate the adaptation to zinc deficiency
Open accessAbstract
Zinc is an essential micronutrient for all living organisms. When facing a shortage in zinc supply, plants adapt by enhancing the zinc uptake capacity. The molecular regulators controlling this adaptation are not known. We present the identification of two closely related members of the Arabidopsis thaliana basic-region leucine-zipper (bZIP) transcription factor gene family, bZIP19 and bZIP23, that regulate the adaptation to low zinc supply. They were identified, in a yeast-one-hybrid screening, to associate to promoter regions of the zinc deficiency-induced ZIP4 gene of the Zrt- and Irt-related protein (ZIP) family of metal transporters. Although mutation of only one of the bZIP genes hardly affects plants, we show that the bzip19 bzip23 double mutant is hypersensitive to zinc deficiency. Unlike the wild type, the bzip19 bzip23 mutant is unable to induce the expression of a small set of genes that constitutes the primary response to zinc deficiency, comprising additional ZIP metal transporter genes. This set of target genes is characterized by the presence of one or more copies of a 10-bp imperfect palindrome in their promoter region, to which both bZIP proteins can bind. The bZIP19 and bZIP23 transcription factors, their target genes, and the characteristic cis zinc deficiency response elements they can bind to are conserved in higher plants. These findings are a significant step forward to unravel the molecular mechanism of zinc homeostasis in plants, allowing the improvement of zinc bio-fortification to alleviate human nutrition problems and phytoremediation strategies to clean contaminated soils.
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- Authors
- Assunção, A. G. L., Herrero, E., Lin, Y.-F., Huettel, B., Talukdar, S., Smaczniak, C., Immink, R. G. H., van Eldik, M., Fiers, M., Schat, H., & Aarts, M. G. M.
- Affiliations
- Wageningen University & Research (NL)
- Max Planck Institute for Plant Breeding ADIS/DNA Core Facility, D-50829 Cologne, Germany;
- Plant Research International, Bioscience, 6708 PB, Wageningen, The Netherlands;
- Centre for BioSystems Genomics (NL)
- Plant Research International, Applied Bioinformatics, 6708 PB, Wageningen, The Netherlands; and
- Vrije Universiteit Amsterdam (NL)
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2010-05-17
- Volume
- 107
- Issue
- 22
- Pages
- 10296-10301
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC2890486
- OpenAlex
- W2132076654
- Cited by
- 379
- References
- 39
- Citations by year
- 2026: 21 · 2025: 31 · 2024: 28 · 2023: 29 · 2022: 33 · 2021: 44 · 2020: 34 · 2019: 20 · 2018: 30 · 2017: 17 · 2016: 19 · 2015: 12 · 2014: 14 · 2013: 21 · 2012: 12
- FWCI
- 18.03
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- VU Research Portal (publishedVersion)
- Socio-Environmental Systems Modeling (acceptedVersion)
- Research at the University of Copenhagen (University of Copenhagen) (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion, other-oa)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Micronutrient Interactions and Effects
- Health Sciences › Nursing › Nutrition and Dietetics › Trace Elements in Health
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- zinc deficiency, zinc homeostasis, bZIP transcription factors, ZIP4, zinc uptake, transcriptional regulation, promoter response elements, zinc biofortification, phytoremediation, Arabidopsis thaliana
- MeSH terms
- Base Sequence, Genetic Complementation Test, Humans, Mutation, Phenotype, Plants, Promoter Regions, Genetic, Zinc, Mutagenesis, Insertional, Conserved Sequence, Genes, Plant, Arabidopsis, DNA, Plant, Two-Hybrid System Techniques, Arabidopsis Proteins, Plants, Genetically Modified, Basic-Leucine Zipper Transcription Factors
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