The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Phosphatidylethanol Formation via Transphosphatidylation by Rat Brain Synaptosomal Phospholipase D
Abstract
Abstract: Phosphatidylethanol (Peth) formation catalyzed by the transphosphatidylation activity of phospholipase D was demonstrated to occur in a rat brain synaptosomal enriched preparation. The optimal pH was determined to be 6.5, and the optimal ethanol concentration was determined to be 0.3–0.4 M with an apparent K m of 0.2 M. Peth formation was barely detectable in the absence of an appropriate activator and several unsaturated fatty acids were found to be effective activators. The concentrations of oleic acid required for maximum activation varied with the concentration of exogenous phosphatidylcholine present in the incubation mixtures. All detergents tested were significantly less active than the unsaturated fatty acids and divalent ions were not required for Peth formation. Phosphatidylcholine was the most effective phosphatidyl donor of the phospho‐lipids tested. Peth forming activity was greatest in the synap‐tic membrane fraction of the various brain subfractions examined. The 12,000 g‐100,000 g paniculate fraction of lung, heart, and adipose tissue had activities similar to that of brain.
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Cites
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Cited by
Phosphatidylcholine breakdown and signal transduction
Exton, J. H. (1994) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(94)90186-4 · Cited by 960
The regulation and cellular functions of phosphatidylcholine hydrolysis
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REGULATION OF EUKARYOTIC PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE C AND PHOSPHOLIPASE D
Singer, W. D., Brown, H. A., & Sternweis, P. C. (1997) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.66.1.475 · Cited by 401
ARADOTTIR, S., ASANOVSKA, G., GJERSS, S., HANSSON, P., & ALLING, C. (2006) · Alcohol and Alcoholism · doi:10.1093/alcalc/agl027 · Cited by 256
Nonoxidative ethanol metabolism in humans—from biomarkers to bioactive lipids
Heier, C., Xie, H., & Zimmermann, R. (2016) · IUBMB Life · doi:10.1002/iub.1569 · Cited by 93
Metadata
- Authors
- Kobayashi, M., & Kanfer, J. N.
- Venue
- Journal of Neurochemistry
- Publisher
- Wiley
- Published
- 1987-05
- Volume
- 48
- Issue
- 5
- Pages
- 1597-1603
- ISSN
- 0022-3042 (ISSN-L)
- 1471-4159
- OpenAlex
- W2019420003
- Cited by
- 340
- References
- 22
- FWCI
- 7.57
- Type
- journal article
- Language
- en
- Open access
- closed
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- Kobayashi, M., & Kanfer, J. N. (1987). Phosphatidylethanol Formation via Transphosphatidylation by Rat Brain Synaptosomal Phospholipase D. Journal of Neurochemistry. https://doi.org/10.1111/j.1471-4159.1987.tb05707.x
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
