Increasing the presence of biofilm and healing delay in a porcine model of MRSA‐infected wounds
Abstract
Data supporting the concept that microbial biofilms are a major cause of non‐healing ulcers remain limited. A porcine model was established where delayed healing resulted from methicillin‐resistant S taphylococcus aureus (MRSA) infection in full‐thickness wounds. At the end of one study a wound remaining open was sampled and a MRSA strain was isolated. This pig‐passaged strain was used as the inoculating strain in several subsequent studies. The resulting MRSA wound infections exhibited a greater, more stable tissue bioburden than seen in studies using the parent strain. Furthermore, wounds infected with the passaged strain experienced a greater delay in healing. To understand whether these changes corresponded to an increased biofilm character of the wound infection, wound biopsy samples from studies using either the parent or passaged MRSA strains were examined microscopically. Evidence of biofilm was observed for both strains, as most samples at a minimum had multiple isolated, dense microcolonies of bacteria. However, the passaged MRSA resulted in bacterial colonies of greater frequency and size that occurred more often in concatenated fashion to generate extended sections of biofilm. These results provide a model case in which increasing biofilm character of a wound infection corresponded with a greater delay in wound healing.
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Continued presence of a high bacterial load in wounds results in a sustained influx of pro-inflammatory cells and increased inflammation, leading to delayed healing.
“In addition, continued presence of a high bacterial load in wounds results in a sustained influx of pro-inflammatory cells and increased inflammation also leading to delayed healing (Fig. 3) (33-35).”
From Wound repair and regeneration: Mechanisms, signaling, and translation (2014) · cites this paper as [35]
Cites
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Wound repair and regeneration: Mechanisms, signaling, and translation
Eming, S. A., Martin, P., & Tomic-Canic, M. (2014) · Science Translational Medicine · doi:10.1126/scitranslmed.3009337 · Cited by 3,562 · see citing works
- Authors
- Roche, E. D., Renick, P. J., Tetens, S. P., Ramsay, S. J., Daniels, E. Q., & Carson, D. L.
- Affiliations
- Research and Development Healthpoint Biotherapeutics Fort Worth TX USA
- University of North Texas Health Science Center (US)
- Corresponding author
- Roche, Eric D.
- Venue
- Wound Repair and Regeneration
- Publisher
- Wiley
- Published
- 2012-06-07
- Volume
- 20
- Issue
- 4
- Pages
- 537-543
- ISSN
- 1067-1927 (ISSN-L)
- 1524-475X
- OpenAlex
- W1595933642
- Cited by
- 56
- References
- 17
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 1 · 2023: 6 · 2022: 7 · 2021: 6 · 2020: 4 · 2019: 3 · 2018: 5 · 2017: 3 · 2016: 4 · 2015: 5 · 2014: 4 · 2013: 3 · 2012: 1
- FWCI
- 4.40
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Wound Repair and Regeneration (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Texas Southwestern Medical Center
- Topics
- Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
- Keywords
- methicillin-resistant Staphylococcus aureus, MRSA biofilm, wound infection, delayed wound healing, chronic ulcers, porcine wound model, full-thickness wounds, microcolonies, biofilm formation
- MeSH terms
- Animals, Female, Inflammation, Microscopy, Electron, Scanning, Staphylococcal Infections, Swine, Time Factors, Wound Healing, Wound Infection, Biofilms, Methicillin-Resistant Staphylococcus aureus
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