Expression of growth differentiation factor 15 is not elevated in individuals with iron deficiency secondary to volunteer blood donation
Open accessAbstract
BACKGROUND: Low serum hepcidin levels provide a physiologic response to iron demand in patients with iron deficiency (ID). Based on a discovery of suppressed hepcidin expression by a cytokine named growth differentiation factor 15 (GDF15), it was hypothesized that GDF15 may suppress hepcidin expression in humans with ID due to blood loss. STUDY DESIGN AND METHODS: To test this hypothesis, GDF15 and hepcidin levels were measured in peripheral blood from subjects with iron-deficient erythropoiesis before and after iron supplementation. RESULTS: Iron variables and hepcidin levels were significantly suppressed in iron-deficient blood donors compared to healthy volunteers. However, ID was not associated with elevated serum levels of GDF15. Instead, iron-deficient subjects' GDF15 levels were slightly lower than those measured in the control group of subjects (307 +/- 90 and 386 +/- 104 pg/mL, respectively). Additionally, GDF15 levels were not significantly altered by iron repletion. CONCLUSIONS: ID due to blood loss is not associated with a significant change in serum levels of GDF15.
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In iron-deficiency anemia, GDF15 serum levels were not elevated.
“In the context of iron-deficiency anemia, Tanno and colleagues found that GDF15 serum levels were not elevated”
From Regulation of erythropoiesis by hypoxia-inducible factors (2013) · cites this paper as [223]
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Cites
Targeting Hypoxia-Inducible Factors for the Treatment of Anemia in Chronic Kidney Disease Patients
Locatelli, F., Fishbane, S. N., Block, G. A., & Macdougall, I. C. (2017) · American Journal of Nephrology · doi:10.1159/000455166 · Cited by 4,834 · see citing works
Nicolas, G., Chauvet, C., Viatte, L., Danan, J. L., Bigard, X., Devaux, I., Beaumont, C., Kahn, A., & Vaulont, S. (2002) · Journal of Clinical Investigation · doi:10.1172/jci0215686 · Cited by 1,214 · see citing works
High levels of GDF15 in thalassemia suppress expression of the iron regulatory protein hepcidin
Tanno, T., Bhanu, N. V., Oneal, P. A., Goh, S.-H., Staker, P., Lee, Y. T., Moroney, J. W., Reed, C. H., Luban, N. L., Wang, R.-H., et al. (2007) · Nature Medicine · doi:10.1038/nm1629 · Cited by 854 · see citing works
Self-citationImmunoassay for human serum hepcidin
Ganz, T., Olbina, G., Girelli, D., Nemeth, E., & Westerman, M. (2008) · Blood · doi:10.1182/blood-2008-02-139915 · Cited by 688 · see citing works
Pinto, J. P., Ribeiro, S., Pontes, H., Thowfeequ, S., Tosh, D., Carvalho, F. D., & Porto, G. (2008) · Blood · doi:10.1182/blood-2007-08-106195 · Cited by 239 · see citing works
Kemna, E., Tjalsma, H., Podust, V. N., & Swinkels, D. W. (2007) · Clinical Chemistry · doi:10.1373/clinchem.2006.079186 · Cited by 222 · see citing works
Iron regulation and erythropoiesis
Nemeth, E. (2008) · Current Opinion in Hematology · doi:10.1097/moh.0b013e3282f73335 · Cited by 175 · see citing works
Tamary, H., Shalev, H., Perez-Avraham, G., Zoldan, M., Levi, I., Swinkels, D. W., Tanno, T., & Miller, J. L. (2008) · Blood · doi:10.1182/blood-2008-06-165738 · Cited by 135 · see citing works
Self-citationRegulation of growth differentiation factor 15 expression by intracellular iron
Lakhal, S., Talbot, N. P., Crosby, A., Stoepker, C., Townsend, A. R. M., Robbins, P. A., Pugh, C. W., Ratcliffe, P. J., & Mole, D. R. (2009) · Blood · doi:10.1182/blood-2008-07-170431 · Cited by 95 · see citing works
A p53-type response element in the GDF15 promoter confers high specificity for p53 activation
Osada, M., Park, H. L., Park, M. J., Liu, J.-W., Wu, G., Trink, B., & Sidransky, D. (2007) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2007.01.089 · Cited by 90 · see citing works
Behavioral, biochemical, and genetic analysis of iron metabolism in high‐intensity blood donors
Mast, A. E., Foster, T. M., Pinder, H. L., Beczkiewicz, C. A., Bellissimo, D. B., Murphy, A., Kovacevic, S., Wroblewski, V. J., & Witcher, D. R. (2008) · Transfusion · doi:10.1111/j.1537-2995.2008.01823.x · Cited by 75 · see citing works
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Ganz, T., & Nemeth, E. (2012) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2012.01.014 · Cited by 1,282 · see citing works
Ganz, T. (2013) · Physiological Reviews · doi:10.1152/physrev.00008.2013 · Cited by 1,122 · see citing works
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Ganz, T. (2011) · Blood · doi:10.1182/blood-2011-01-258467 · Cited by 962 · see citing works
Regulation of erythropoiesis by hypoxia-inducible factors
Haase, V. H. (2013) · Blood Reviews · doi:10.1016/j.blre.2012.12.003 · Cited by 787 · see citing works
Hypoxia-inducible factor regulates hepcidin via erythropoietin-induced erythropoiesis
Liu, Q., Davidoff, O., Niss, K., & Haase, V. H. (2012) · Journal of Clinical Investigation · doi:10.1172/jci63924 · Cited by 328 · see citing works
Breit, S. N., Johnen, H., Cook, A. D., Tsai, V. W. W., Mohammad, M. G., Kuffner, T., Zhang, H. P., Marquis, C. P., Jiang, L., Lockwood, G., et al. (2011) · Growth Factors · doi:10.3109/08977194.2011.607137 · Cited by 247 · see citing works
- Authors
- Tanno, T., Rabel, A., Lee, Y. T., Yau, Y. Y., Leitman, S. F., & Miller, J. L.
- Affiliations
- National Institutes of Health (US)
- Corresponding author
- Miller, Jeffery L.
- Venue
- Transfusion
- Publisher
- Wiley
- Published
- 2010-07
- Volume
- 50
- Issue
- 7
- Pages
- 1532-1535
- ISSN
- 0041-1132 (ISSN-L)
- 1537-2995
- PMC
- PMC3282986
- OpenAlex
- W2165481069
- Cited by
- 34
- References
- 11
- Citations by year
- 2023: 2 · 2022: 1 · 2021: 2 · 2020: 3 · 2019: 1 · 2018: 1 · 2017: 3 · 2015: 5 · 2014: 2 · 2013: 8 · 2012: 3
- FWCI
- 2.81
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Transfusion (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- National Institute of Diabetes and Digestive and Kidney Diseases (Z01DK025094)
- Topics
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Health Sciences › Medicine › Rheumatology › GDF15 and Related Biomarkers
- Health Sciences › Medicine › Hematology › Erythropoietin and Anemia Treatment
- Keywords
- growth differentiation factor 15, hepcidin, iron deficiency, blood donation, iron deficiency anemia, iron repletion, iron-deficient erythropoiesis, blood loss, cytokines
- MeSH terms
- Iron Deficiencies, Blood Donors, Ferritins, Humans, Transferrin, Antimicrobial Cationic Peptides, Growth Differentiation Factor 15, Hepcidins
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