In vitro fermentation of oat and barley derived β-glucans by human faecal microbiota: In vitro fermentation of cereal β-glucans
Open accessAbstract
Fermentation of beta-glucan fractions from barley [average molecular mass (MM), of 243, 172, and 137 kDa] and oats (average MM of 230 and 150 kDa) by the human faecal microbiota was investigated. Fractions were supplemented to pH-controlled anaerobic batch culture fermenters inoculated with human faecal samples from three donors, in triplicate, for each substrate. Microbiota changes were monitored by fluorescent in situ hybridization; groups enumerated were: Bifidobacterium genus, Bacteroides and Prevotella group, Clostridium histolyticum subgroup, Ruminococcus-Eubacterium-Clostridium (REC) cluster, Lactobacillus-Enterococcus group, Atopobium cluster, and clostridial cluster IX. Short-chain fatty acids and lactic acid were measured by HPLC. The C. histolyticum subgroup increased significantly in all vessels and clostridial cluster IX maintained high populations with all fractions. The Bacteroides-Prevotella group increased with all but the 243-kDa barley and 230-kDa oat substrates. In general beta-glucans displayed no apparent prebiotic potential. The SCFA profile (51 : 32 : 17; acetate : propionate : butyrate) was considered propionate-rich. In a further study a beta-glucan oligosaccharide fraction was produced with a degree of polymerization of 3-4. This fraction was supplemented to small-scale faecal batch cultures and gave significant increases in the Lactobacillus-Enterococcus group; however, the prebiotic potential of this fraction was marginal compared with that of inulin.
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Fermentation of arabinoxylan results in greater production of butyrate compared to fermentation of β-glucan.
“The fermentation of RS results in the production of higher levels of butyrate compared to NSPs (Topping and Clifton, 2001; Topping et al., 2008), while fermentation of arabinoxylan results in greater production of butyrate compared to fermentation of β-glucan (Hughes et al., 2007; 2008).”
From Role of polysaccharides in food, digestion, and health (2017) · cites this paper as Hughes et al., 2008
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“The fermentation of RS results in the production of higher levels of butyrate compared to NSPs (Topping and Clifton, 2001; Topping et al., 2008), while fermentation of arabinoxylan results in greater production of butyrate compared to fermentation of b-glucan (Hughes et al., 2007; 2008).”
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- Authors
- Hughes, S. A., Shewry, P. R., Gibson, G. R., McCleary, B. V., & Rastall, R. A.
- Affiliations
- University of Reading (GB)
- Rothamsted Research (GB)
- Corresponding author
- Rastall, Robert A.
- Venue
- FEMS Microbiology Ecology
- Publisher
- Oxford University Press (OUP)
- Published
- 2008-04-21
- Volume
- 64
- Issue
- 3
- Pages
- 482-493
- ISSN
- 0168-6496 (ISSN-L)
- 1574-6941
- OpenAlex
- W2150159510
- Cited by
- 276
- References
- 1
- Citations by year
- 2026: 9 · 2025: 8 · 2024: 10 · 2023: 15 · 2022: 18 · 2021: 34 · 2020: 17 · 2019: 16 · 2018: 19 · 2017: 21 · 2016: 14 · 2015: 20 · 2014: 18 · 2013: 12 · 2012: 10
- FWCI
- 4.64
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEMS Microbiology Ecology (publishedVersion)
- PubMed (publishedVersion)
- Rothamsted Repository (Rothamsted Repository) (submittedVersion)
- Funders
- Directorate for Biological Sciences
- Biotechnology and Biological Sciences Research Council (BBS/E/C/00004953)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Nursing › Nutrition and Dietetics › Microbial Metabolites in Food Biotechnology
- Health Sciences › Nursing › Nutrition and Dietetics › Food composition and properties
- Keywords
- β-glucan, oat β-glucan, barley β-glucan, human faecal microbiota, in vitro fermentation, short-chain fatty acids, prebiotic activity, Bifidobacterium, Clostridium histolyticum, inulin, anaerobic batch culture
- MeSH terms
- Adult, Bacteria, Hordeum, Chromatography, High Pressure Liquid, DNA, Bacterial, Fatty Acids, Feces, Female, Fermentation, Humans, Male, Molecular Weight, Colony Count, Microbial, In Situ Hybridization, Fluorescence, Avena, beta-Glucans
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