Resistance to amphotericin B associated with defective sterol Î8â7isomerase in a Cryptococcus neoformans strain from an AIDS patient
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Two Cryptococcus neoformans strains isolated from an AIDS patient were investigated, a pretreatment isolate (CN1) and a second isolate (CN3) following failure of fluconazole and amphotericin B treatment. No difference in fluconazole sensitivity, but relative resistance to amphotericin B was observed for CN3. The sterol composition of CN3 indicated a defect in sterol delta 8-->7 isomerase in this strain and depletion of ergosterol, the major sterol of the CN1.
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In C. neoformans, only one case of a resistant clinical isolate harbouring a mutation in ERG2 has been documented to date.
“In C. neoformans, despite the use of amphotericin B as the first line of defence against meningeal cryptococcosis, only one case of a resistant clinical isolate harbouring a mutation in ERG2 has been documented to date48.”
From Molecular mechanisms governing antifungal drug resistance (2023) · cites this paper as [48]
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- Authors
- Kelly, S. L., Lamb, D. C., Taylor, M., Corran, A. J., Baldwin, B. C., & Powderly, W. G.
- Affiliations
- University of Sheffield (GB)
- AstraZeneca (United Kingdom) (GB)
- Washington University in St. Louis (US)
- Corresponding author
- Kelly, Steven L.
- Venue
- FEMS Microbiology Letters
- Publisher
- Oxford University Press (OUP)
- Published
- 1994-09
- Volume
- 122
- Issue
- 1-2
- Pages
- 39-42
- ISSN
- 0378-1097 (ISSN-L)
- 1574-6968
- OpenAlex
- W2120392598
- Cited by
- 133
- References
- 6
- Citations by year
- 2026: 4 · 2025: 4 · 2024: 5 · 2023: 11 · 2022: 3 · 2021: 5 · 2020: 7 · 2019: 4 · 2018: 2 · 2017: 6 · 2016: 4 · 2015: 3 · 2014: 7 · 2013: 4 · 2012: 2
- FWCI
- 4.12
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEMS Microbiology Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Wellcome Trust
- Topics
- Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- Cryptococcus neoformans, amphotericin B resistance, ergosterol depletion, sterol composition, fluconazole resistance, antifungal resistance
- MeSH terms
- Acquired Immunodeficiency Syndrome, Amphotericin B, Cryptococcus neoformans, Drug Resistance, Microbial, Humans, Microbial Sensitivity Tests, Mutation, Steroid Isomerases
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